Detailed view for Bm1_18320

Basic information

TDR Targets ID: 234206
Brugia malayi, hypothetical protein
Source Database / ID:  GenBank

pI: 6.5667 | Length (AA): 586 | MW (Da): 67204 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00632   HECT-domain (ubiquitin-transferase)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0004842   ubiquitin-protein ligase activity  
GO:0016881   acid-amino acid ligase activity  
GO:0006464   protein modification process  

Metabolic Pathways

Ubiquitin mediated proteolysis (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
145 580 3olm (A) 385 803 23.00 0 1 0.958227 0.25
147 176 2dmv (A) 6 35 13.00 0.51 0.01 0.102395 0.69
158 586 5lp8 (B) 3952 4374 27.00 0 1 1.01328 -0.02
194 581 5hps (A) 543 917 26.00 0 1 0.962316 -0.21
480 577 3pt3 (A) 2694 2791 34.00 0 0.99 0.437036 0.06

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127286)

Species Accession Gene Product
Arabidopsis thaliana AT3G17205   E3 ubiquitin-protein ligase UPL6
Arabidopsis thaliana AT3G53090   E3 ubiquitin-protein ligase UPL7
Babesia bovis BBOV_III011100   HECT domain containing protein
Brugia malayi Bm1_16015   ubiquitin-protein ligase UBE3B
Brugia malayi Bm1_18320   hypothetical protein
Candida albicans CaO19.1173   similar to S. cerevisiae HUL5 (YGL141W) ubiquitin protein ligase
Caenorhabditis elegans CELE_Y39A1C.2   Protein OXI-1
Caenorhabditis elegans CELE_D2085.4   Protein D2085.4
Cryptosporidium hominis Chro.80140   hypothetical protein
Cryptosporidium parvum cgd8_1200   E3A like HECT domain containing ubiquitin protein ligase
Drosophila melanogaster Dmel_CG5087   CG5087 gene product from transcript CG5087-RA
Drosophila melanogaster Dmel_CG3356   CG3356 gene product from transcript CG3356-RA
Echinococcus granulosus EgrG_001199700   ubiquitin protein ligase
Echinococcus granulosus EgrG_001199500   ubiquitin protein ligase E3B
Echinococcus granulosus EgrG_000633700   ubiquitin protein ligase
Echinococcus multilocularis EmuJ_001199700   ubiquitin protein ligase
Echinococcus multilocularis EmuJ_001199500   ubiquitin protein ligase E3B
Echinococcus multilocularis EmuJ_000633700   ubiquitin protein ligase
Homo sapiens ENSG00000151148   ubiquitin protein ligase E3B
Homo sapiens ENSG00000009335   ubiquitin protein ligase E3C
Leishmania braziliensis LbrM.30.1030   ubiquitin-protein ligase, putative
Leishmania donovani LdBPK_300970.1   ubiquitin-protein ligase, putative
Leishmania infantum LinJ.30.0970   ubiquitin-protein ligase, putative
Leishmania major LmjF.30.0910   ubiquitin-protein ligase, putative
Leishmania mexicana LmxM.29.0910   ubiquitin-protein ligase, putative
Loa Loa (eye worm) LOAG_06133   ubiquitin-protein ligase UBE3B
Loa Loa (eye worm) LOAG_08325   hypothetical protein
Mus musculus ENSMUSG00000039000   ubiquitin protein ligase E3C
Mus musculus ENSMUSG00000029577   ubiquitin protein ligase E3B
Neospora caninum NCLIV_007280   hypothetical protein
Oryza sativa 4337876   Os05g0159000
Saccharomyces cerevisiae YGL141W   ubiquitin-ubiquitin ligase HUL5
Schistosoma japonicum Sjp_0064980   ko:K10588 ubiquitin-protein ligase E3 B, putative
Schistosoma japonicum Sjp_0064990   Ubiquitin-protein ligase E3B, putative
Schistosoma mansoni Smp_137040   ubiquitin-protein ligase
Schmidtea mediterranea mk4.041028.00   Ubiquitin-protein ligase E3C
Schmidtea mediterranea mk4.011843.00   Ubiquitin-protein ligase E3B
Trypanosoma brucei gambiense Tbg972.6.2130   ubiquitin-protein ligase, putative
Trypanosoma brucei Tb927.6.2370   ubiquitin-protein ligase, putative
Trypanosoma congolense TcIL3000_6_1870   ubiquitin-protein ligase, putative
Trypanosoma congolense TcIL3000_0_38710   ubiquitin-protein ligase, putative
Trypanosoma cruzi TcCLB.509053.10   ubiquitin-protein ligase, putative
Trypanosoma cruzi TcCLB.509965.350   ubiquitin-protein ligase, putative
Toxoplasma gondii TGME49_275630   HECT-domain (ubiquitin-transferase) domain-containing protein
Theileria parva TP02_0025   ubiquitin-protein ligase, putative

Essentiality

Bm1_18320 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.2370 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.2370 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.2370 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.2370 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_275630 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Bm1_18320 (Brugia malayi), hypothetical protein
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