Detailed view for Bm1_41035
Basic information
Brugia malayi, Zinc metalloproteinase toh-2 precursor
Source Database / ID: GenBank
pI: 7.5252 |
Length (AA): 482 |
MW (Da): 55814 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Network
Gene Ontology
GO:0008270 zinc ion binding
GO:0008237 metallopeptidase activity
GO:0004222 metalloendopeptidase activity
GO:0006508 proteolysis
Metabolic Pathways
Structural information
Modbase 3D models:
There are 4 models calculated for this protein. More info on
these models, including the
models themselves is available at:
Modbase
| Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
|---|---|---|---|---|---|---|---|---|---|
| 31 | 226 | 3edh (A) | 3 | 194 | 35.00 | 0 | 1 | 0.890139 | -0.85 |
| 31 | 368 | 4gwn (A) | 64 | 392 | 22.00 | 0 | 1 | 0.889745 | 0.79 |
| 229 | 264 | 1f7e (A) | 48 | 83 | 42.00 | 0 | 0.96 | 0.584189 | -0.76 |
| 237 | 266 | 2ygq (A) | 186 | 213 | 50.00 | 0.062 | 0.99 | 0.663841 | -0.78 |
Help me make sense of these data.
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
Expression
Orthologs
Ortholog group members (OG5_179061)
| Species | Accession | Gene Product |
|---|---|---|
| Brugia malayi | Bm1_41035 | Zinc metalloproteinase toh-2 precursor |
| Caenorhabditis elegans | CELE_R151.5 | Protein DPY-31, isoform A |
| Loa Loa (eye worm) | LOAG_02277 | DPY-31 protein |
Essentiality
| Gene/Ortholog | Organism | Phenotype | Source Study |
|---|---|---|---|
| CELE_R151.5 | Caenorhabditis elegans | embryonic lethal | wormbase |
-
blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930 -
keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection -
neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs -
nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource -
wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170 -
gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84 -
alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924 -
yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database -
shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
Phenotypes and Validation (curated)
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Annotated validation
Associated compounds / Druggability
Known modulators for this target
| Compound | Source | Reference |
|---|---|---|
| ChEMBL23 | References |
Predicted associations
By orthology with druggable targets
By sequence similarity to non orthologous druggable targets
Obtained from network model
Assayability
Assay information
- ChEMBL
- Inhibition of recombinant Brugia malayi DPY-31 astacin domain expressed in Escherichia coli using Suc-Ala-Ala-Ala-pNA as substrate preincubated for 3 hrs followed by substrate addition measured after 3 hrs by microplate reader assay
Reagent availability
Bibliographic References