Detailed view for LmjF.36.6390

Basic information

TDR Targets ID: 23617
Leishmania major, methylenetetrahydrofolate reductase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.4234 | Length (AA): 309 | MW (Da): 34752 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02219   Methylenetetrahydrofolate reductase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004489   methylenetetrahydrofolate reductase (NADPH) activity  
GO:0055114   oxidation reduction  
GO:0006555   methionine metabolic process  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 306 1v93 (A) 3 289 35.00 0 1 1.41 -1.59
3 306 3apt (A) 1 289 35.00 0 1 1.51462 -1.41
11 306 3fst (A) 17 292 30.00 0 1 1.40873 -1.51

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128744)

Species Accession Gene Product
Arabidopsis thaliana AT3G59970   methylenetetrahydrofolate reductase 1
Arabidopsis thaliana AT2G44160   methylenetetrahydrofolate reductase 2
Brugia malayi Bm1_35485   Probable methylenetetrahydrofolate reductase
Candida albicans CaO19.288   one of two potential methylenetetrahydrofolate reductase genes similar to S. cerevisiae MET13 (YGL125W) and MET12 (YPL023C)
Candida albicans CaO19.7920   one of two potential methylenetetrahydrofolate reductase genes similar to S. cerevisiae MET13 (YGL125W) and MET12 (YPL023C)
Caenorhabditis elegans CELE_C06A8.1   Protein MTHF-1, isoform B
Dictyostelium discoideum DDB_G0279137   methylenetetrahydrofolate reductase
Drosophila melanogaster Dmel_CG7560   CG7560 gene product from transcript CG7560-RA
Escherichia coli b3941   5,10-methylenetetrahydrofolate reductase
Homo sapiens ENSG00000177000   methylenetetrahydrofolate reductase (NAD(P)H)
Leishmania braziliensis LbrM.35.6720   methylenetetrahydrofolate reductase, putative
Leishmania donovani LdBPK_366670.1   methylenetetrahydrofolate reductase, putative
Leishmania infantum LinJ.36.6670   methylenetetrahydrofolate reductase, putative
Leishmania major LmjF.36.6390   methylenetetrahydrofolate reductase, putative
Leishmania mexicana LmxM.36.6390   methylenetetrahydrofolate reductase, putative
Loa Loa (eye worm) LOAG_08597   methylenetetrahydrofolate reductase
Mus musculus ENSMUSG00000029009   5,10-methylenetetrahydrofolate reductase
Oryza sativa 4334554   Os03g0815200
Saccharomyces cerevisiae YGL125W   methylenetetrahydrofolate reductase (NAD(P)H) MET13
Schmidtea mediterranea mk4.003253.01   Methylenetetrahydrofolate reductase
Schmidtea mediterranea mk4.003253.00  
Schmidtea mediterranea mk4.028989.01   Methylenetetrahydrofolate reductase

Essentiality

LmjF.36.6390 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b3941 Escherichia coli non-essential goodall
CELE_C06A8.1 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Lmaj006157AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Lmaj006157; Recombinant protein: full-length; Source: L major; methylenetetrahydrofolate reductase putative ;

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.36.6390 (Leishmania major), methylenetetrahydrofolate reductase, putative
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