Detailed view for LmjF.34.2480

Basic information

TDR Targets ID: 23658
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6507 | Length (AA): 1069 | MW (Da): 119292 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
82 952 1tr2 (A) 128 1055 13.00 0.000033 0.96 0.83 0.48
142 230 3q8t (A) 178 260 36.00 0.051 0.11 0.382055 -0.3
213 412 3fb2 (B) 1339 1544 19.00 0 0.81 0.446691 -1.14
354 539 4uos (A) 1 186 25.00 0.78 0.88 0.573794 -1.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_131340)

Species Accession Gene Product
Echinococcus granulosus EgrG_000714600   coiled coil domain containing protein 146
Echinococcus granulosus EgrG_000390800   coiled coil domain containing protein 146
Echinococcus multilocularis EmuJ_000714600   coiled coil domain containing protein 146
Echinococcus multilocularis EmuJ_000390800   coiled coil domain containing protein 146
Giardia lamblia GL50803_102248   Coiled-coil protein
Homo sapiens ENSG00000135205   coiled-coil domain containing 146
Leishmania braziliensis LbrM.20.2010   hypothetical protein, conserved
Leishmania donovani LdBPK_342280.1   hypothetical protein, conserved
Leishmania infantum LinJ.34.2280   hypothetical protein, conserved
Leishmania major LmjF.34.2480   hypothetical protein, conserved
Leishmania mexicana LmxM.33.2480   hypothetical protein, conserved
Mus musculus ENSMUSG00000064280   coiled-coil domain containing 146
Neospora caninum NCLIV_017760   hypothetical protein, conserved
Schistosoma japonicum Sjp_0046860   IPR009053,Prefoldin,domain-containing
Schistosoma mansoni Smp_174770   coiled-coil flagellar protein
Schmidtea mediterranea mk4.000237.05   Putative coiled-coil flagellar protein
Trypanosoma brucei gambiense Tbg972.4.2070   hypothetical protein, conserved
Trypanosoma brucei Tb927.4.2140   Component of motile flagella 8
Trypanosoma congolense TcIL3000_4_1870   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506559.500   hypothetical protein, conserved
Toxoplasma gondii TGME49_242760   hypothetical protein
Trichomonas vaginalis TVAG_174030   DNA repair protein Rad-50, putative
Trichomonas vaginalis TVAG_286940   DNA double-strand break repair Rad50 ATPase, putative
Trichomonas vaginalis TVAG_300900   golgin IMH1, putative

Essentiality

LmjF.34.2480 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.2140 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.2140 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.2140 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.2140 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_242760 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.34.2480 (Leishmania major), hypothetical protein, conserved
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