Detailed view for LmjF.34.0470

Basic information

TDR Targets ID: 23698
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.4153 | Length (AA): 716 | MW (Da): 76138 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00400   WD domain, G-beta repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 116 1p22 (A) 368 469 27.00 0.0000000012 0.26 0.32 -0.16
9 379 1got (B) 37 340 23.00 0.0000000000016 1 0.59 0.29
13 103 5gxh (A) 630 711 37.00 0.084 0.65 0.452595 -0.29
25 393 2ymu (A) 137 551 33.00 0 1 0.608863 0.98
47 378 2ovr (B) 2393 2649 30.00 0.0000000094 1 0.444187 0.71
51 172 2aq5 (A) 56 171 30.00 0.022 0.92 0.432891 0.31
195 382 3fm0 (A) 59 225 34.00 0.00014 0.57 0.40507 0.56
634 702 5j2l (B) 8 76 17.00 0.47 0.01 0.339169 -1.09

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_130011)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG10191   Proteome of centrioles 1
Echinococcus granulosus EgrG_000333600   POC1 centriolar protein A
Echinococcus multilocularis EmuJ_001007600   WD repeat containing protein 51A
Echinococcus multilocularis EmuJ_000333600   POC1 centriolar protein A
Giardia lamblia GL50803_33762   WD-40 repeat protein
Homo sapiens ENSG00000164087   POC1 centriolar protein A
Homo sapiens ENSG00000139323   POC1 centriolar protein B
Leishmania braziliensis LbrM.20.0450   hypothetical protein, conserved
Leishmania donovani LdBPK_340490.1   WD domain, G-beta repeat, putative
Leishmania infantum LinJ.34.0490   hypothetical protein, conserved
Leishmania major LmjF.34.0470   hypothetical protein, conserved
Leishmania mexicana LmxM.33.0470   hypothetical protein, conserved
Mus musculus ENSMUSG00000023345   POC1 centriolar protein homolog A (Chlamydomonas)
Mus musculus ENSMUSG00000019952   POC1 centriolar protein homolog B (Chlamydomonas)
Schistosoma japonicum Sjp_0213770   Uncharacterized WD repeat-containing protein alr2800, putative
Schistosoma mansoni Smp_079610   G-protein beta wd-40 repeats containing protein
Schmidtea mediterranea mk4.006590.03   POC1 centriolar protein homolog B
Schmidtea mediterranea mk4.013333.00   POC1 centriolar protein homolog B
Trypanosoma brucei gambiense Tbg972.10.3560   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.2860   POC1 centriolar protein homolog
Trypanosoma congolense TcIL3000_10_2470   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.504105.110   POC1 centriolar protein homolog
Trichomonas vaginalis TVAG_140120   WD-repeat protein, putative
Trichomonas vaginalis TVAG_026210   platelet-activating factor acetylhydrolase isoform 1B alpha subunit, putative
Trichomonas vaginalis TVAG_286080   WD-repeat protein, putative

Essentiality

LmjF.34.0470 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.2860 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.2860 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.2860 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.2860 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LmjF.34.0470 (Leishmania major), hypothetical protein, conserved
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