Detailed view for LmjF.33.2610

Basic information

TDR Targets ID: 23741
Leishmania major, mitochondrial processing peptidase alpha subunit, putative,metallo-peptidase, Clan ME, Family M16
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.0002 | Length (AA): 483 | MW (Da): 53112 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05193   Peptidase M16 inactive domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0046872   metal ion binding  
GO:0008270   zinc ion binding  
GO:0004222   metalloendopeptidase activity  
GO:0003824   catalytic activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 482 1q2l (A) 28 508 9.00 0 1 0.97 -0.11
15 472 1hr6 (A) 14 462 26.00 0 1 1.28 -0.95
18 472 1hr6 (B) 24 462 18.00 0 1 1.26 -1.1
8 470 2a06 (A) 2 441 18.00 0 1 1.21069 -0.39
14 466 2a06 (B) 18 439 22.00 0 1 1.21099 -0.4
18 480 1hr6 (A) 17 470 27.00 0 1 1.24729 -0.16

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127941)

Species Accession Gene Product
Arabidopsis thaliana AT1G51980   putative mitochondrial-processing peptidase subunit alpha-1
Arabidopsis thaliana AT3G16480   mitochondrial processing peptidase alpha subunit
Babesia bovis BBOV_III003850   mitochondrial processing peptidase alpha subunit, putative
Brugia malayi Bm1_13875   Peptidase M16 inactive domain containing protein
Candida albicans CaO19.13674   subunit of the mitochondrial processing protease
Candida albicans CaO19.6295   subunit of the mitochondrial processing protease
Caenorhabditis elegans CELE_Y71G12B.24   Protein MPPA-1
Cryptosporidium hominis Chro.70239   mitochondrial processing peptidase alpha subunit
Cryptosporidium parvum cgd7_2080   mitochondrial processing peptidase, insulinase like metalloprotease
Dictyostelium discoideum DDB_G0274809   mitochondrial processing peptidase alpha subunit
Dictyostelium discoideum DDB_G0290997   peptidase M16 family protein
Drosophila melanogaster Dmel_CG8728   CG8728 gene product from transcript CG8728-RA
Echinococcus granulosus EgrG_000058800   mitochondrial processing peptidase
Echinococcus multilocularis EmuJ_000058800   mitochondrial processing peptidase
Homo sapiens ENSG00000165688   peptidase (mitochondrial processing) alpha
Leishmania braziliensis LbrM.33.2890   mitochondrial processing peptidase alpha subunit, putative,metallo-peptidase, Clan ME, Family M16
Leishmania donovani LdBPK_332750.1   Mitochondrial-processing peptidase subunit alpha
Leishmania infantum LinJ.33.2750   mitochondrial processing peptidase alpha subunit, putative,metallo-peptidase, Clan ME, Family M16
Leishmania major LmjF.33.2610   mitochondrial processing peptidase alpha subunit, putative,metallo-peptidase, Clan ME, Family M16
Leishmania mexicana LmxM.32.2610   mitochondrial processing peptidase alpha subunit, putative,metallo-peptidase, Clan ME, Family M16
Loa Loa (eye worm) LOAG_01204   peptidase M16 inactive domain-containing protein
Mus musculus ENSMUSG00000026926   peptidase (mitochondrial processing) alpha
Neospora caninum NCLIV_022220   hypothetical protein
Oryza sativa 4327198   Os01g0739000
Oryza sativa 4339368   Os05g0524300
Oryza sativa 4324473   Os01g0966300
Plasmodium berghei PBANKA_1237900   mitochondrial-processing peptidase subunit alpha, putative
Plasmodium falciparum PF3D7_0523100   mitochondrial-processing peptidase subunit alpha, putative
Plasmodium knowlesi PKNH_1009800   mitochondrial-processing peptidase subunit alpha, putative
Plasmodium vivax PVX_080095   mitochondrial processing peptidase alpha subunit, putative
Plasmodium yoelii PY00244   mitochondrial processing peptidase alpha subunit homolog
Saccharomyces cerevisiae YHR024C   Mas2p
Schistosoma japonicum Sjp_0203980   ko:K01412 mitochondrial processing peptidase [EC3.4.24.64], putative
Schistosoma mansoni Smp_094050.1   mitochondrial processing peptidase non-peptidase alpha subunit (M16 family)
Schistosoma mansoni Smp_094050.2   mitochondrial processing peptidase non-peptidase alpha subunit (M16 family)
Schmidtea mediterranea mk4.000787.04   Mitochondrial-processing peptidase subunit alpha
Trypanosoma brucei gambiense Tbg.972.2.2270   mitochondrial processing peptidase alpha subunit, putative,metallo-peptidase, Clan ME, Family M16, putative
Trypanosoma brucei Tb927.2.4110   Mitochondrial-processing peptidase subunit alpha
Trypanosoma congolense TcIL3000_0_19100   mitochondrial processing peptidase alpha subunit, putative
Trypanosoma congolense TcIL3000_2_630   Mitochondrial-processing peptidase subunit alpha
Trypanosoma cruzi TcCLB.508441.80   Mitochondrial-processing peptidase subunit alpha
Trypanosoma cruzi TcCLB.507547.30   metallo-peptidase, Clan ME, Family M16
Toxoplasma gondii TGME49_202680   peptidase M16, alpha subunit, putative
Theileria parva TP02_0218   ubiquinol-cytochrome C reductase complex core protein II, mitochondrial precursor, putative
Trichomonas vaginalis TVAG_119710   Clan ME, family M16, insulinase-like metallopeptidase

Essentiality

LmjF.33.2610 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.4110 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.4110 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.2.4110 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.2.4110 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YHR024C Saccharomyces cerevisiae inviable yeastgenome
TGME49_202680 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.33.2610 (Leishmania major), mitochondrial processing peptidase alpha subunit, putative,metallo-peptidase, Clan ME, Family M16
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