Detailed view for Bm1_48360

Basic information

TDR Targets ID: 238089
Brugia malayi, PP3111 protein
Source Database / ID:  GenBank

pI: 8.4154 | Length (AA): 513 | MW (Da): 58665 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01207   Dihydrouridine synthase (Dus)

Gene Ontology

Mouse over links to read term descriptions.
GO:0050660   FAD binding  
GO:0017150   tRNA dihydrouridine synthase activity  
GO:0003824   catalytic activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  
GO:0008033   tRNA processing  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 285 1ox5 (B) 226 535 9.00 0 0.1 0.491009 0.79
11 285 3hgj (A) 38 330 15.00 0 0.88 0.606262 0.46
60 353 1vhn (A) 5 308 24.00 0 1 0.869299 -0.54
61 359 4wfs (A) 13 329 22.00 0 1 0.757046 -0.16
61 219 3b0p (A) 5 167 31.00 0 1 0.656741 -0.13
191 261 2nv1 (A) 21 93 24.00 0 0.9 0.422602 -0.52
436 487 4r7e (A) 637 692 33.00 0.2 0.5 0.386165 0.19

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127749)

Species Accession Gene Product
Arabidopsis thaliana AT5G67220   FMN-linked oxidoreductases superfamily protein
Babesia bovis BBOV_IV004780   dihydrouridine synthase, putative
Brugia malayi Bm1_48360   PP3111 protein
Candida albicans CaO19.1404   likely tRNA dihydrouridine synthase similar to S. cerevisiae DUS1 (YML080W) dihydrouridine synthase specific for pre-tRNA-Phe
Candida albicans CaO19.8982   likely tRNA dihydrouridine synthase similar to S. cerevisiae DUS1 (YML080W) dihydrouridine synthase specific for pre-tRNA-Phe
Caenorhabditis elegans CELE_F36A2.2   Protein F36A2.2
Cryptosporidium hominis Chro.10384   ENSANGP00000019228
Cryptosporidium parvum cgd1_3440   Dus1p, tRNA dihydrouridine synthase
Dictyostelium discoideum DDB_G0278803   tRNA-dihydrouridine synthase 1-like protein
Drosophila melanogaster Dmel_CG3645   CG3645 gene product from transcript CG3645-RA
Echinococcus granulosus EgrG_000616200   tRNA dihydrouridine synthase 1
Entamoeba histolytica EHI_164780   dihydrouridine synthase (Dus) family protein
Echinococcus multilocularis EmuJ_000616200   tRNA dihydrouridine synthase 1
Giardia lamblia GL50803_5213   tRNA dihydrouridine synthase, putative
Homo sapiens 64118   dihydrouridine synthase 1-like (S. cerevisiae)
Leishmania braziliensis LbrM.31.2160   dihydrouridine synthase (Dus), putative
Leishmania donovani LdBPK_311970.1   dihydrouridine synthase (Dus), putative
Leishmania infantum LinJ.31.1970   dihydrouridine synthase (Dus), putative
Leishmania major LmjF.31.1930   dihydrouridine synthase (Dus), putative
Leishmania mexicana LmxM.30.1930   dihydrouridine synthase (Dus), putative
Loa Loa (eye worm) LOAG_05027   hypothetical protein
Mus musculus ENSMUSG00000025155   dihydrouridine synthase 1-like (S. cerevisiae)
Neospora caninum NCLIV_022120   dihydrouridine synthase domain-containing protein, putative
Oryza sativa 4342049   Os06g0712900
Plasmodium berghei PBANKA_1033600   tRNA-dihydrouridine synthase, putative
Plasmodium falciparum PF3D7_1408900   tRNA-dihydrouridine synthase, putative
Plasmodium knowlesi PKNH_1349500   tRNA-dihydrouridine synthase, putative
Plasmodium vivax PVX_086005   tRNA-dihydrouridine synthase, putative
Plasmodium yoelii PY01376   Drosophila melanogaster CG3645 gene product
Saccharomyces cerevisiae YML080W   Dus1p
Schistosoma japonicum Sjp_0200030   ko:K05542 tRNA-dihydrouridine synthase 1, putative
Schistosoma mansoni Smp_096200   tRNA-dihydrouridine synthase
Schmidtea mediterranea mk4.002092.03  
Trypanosoma brucei gambiense Tbg972.8.7840   tRNA-dihydrouridine synthase 1, putative
Trypanosoma brucei Tb927.8.7570   tRNA-dihydrouridine synthase 1, putative
Trypanosoma congolense TcIL3000_8_7960   tRNA-dihydrouridine synthase 1, putative
Trypanosoma congolense TcIL3000_8_7820   dihydrouridine synthase (Dus), putative
Trypanosoma congolense TcIL3000_0_23020   tRNA-dihydrouridine synthase 1, putative
Trypanosoma cruzi TcCLB.504173.50   dihydrouridine synthase (Dus), putative
Trypanosoma cruzi TcCLB.506107.20   dihydrouridine synthase (Dus), putative
Toxoplasma gondii TGME49_202790   dihydrouridine synthase (dus) protein
Theileria parva TP01_0269   hypothetical protein, conserved
Trichomonas vaginalis TVAG_353310   tRNA-dihydrouridine synthase, putative
Trichomonas vaginalis TVAG_021320   tRNA-dihydrouridine synthase, putative
Trichomonas vaginalis TVAG_251740   tRNA-dihydrouridine synthase, putative
Trichomonas vaginalis TVAG_323330   tRNA-dihydrouridine synthase, putative

Essentiality

Bm1_48360 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.7570 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.7570 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.7570 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.7570 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1033600 Plasmodium berghei Dispensable plasmo
TGME49_202790 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier Bm1_48360 (Brugia malayi), PP3111 protein
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