Detailed view for LmjF.27.0020

Basic information

TDR Targets ID: 23853
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.0934 | Length (AA): 1029 | MW (Da): 115039 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 7

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF09773   Meckelin (Transmembrane protein 67)

Gene Ontology

Mouse over links to read term descriptions.
GO:0060271   GO:cilium assembly  

GO:0036038   GO:MKS complex  

GO:0010826   GO:negative regulation of centrosome duplication  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
48 164 3lmf (A) 8 112 15.00 0 0.05 0.172603 -0.49

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_130424)

Species Accession Gene Product
Brugia malayi Bm1_22085   MGC26979 protein
Caenorhabditis elegans CELE_F35D2.4   Protein MKS-3
Drosophila melanogaster Dmel_CG15923   CG15923 gene product from transcript CG15923-RB
Homo sapiens ENSG00000164953   transmembrane protein 67
Leishmania donovani LdBPK_270020.1   Meckelin (Transmembrane protein 67), putative
Leishmania infantum LinJ.27.0020   hypothetical protein, conserved
Leishmania major LmjF.27.0020   hypothetical protein, conserved
Leishmania mexicana LmxM.27.0020   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_15915   hypothetical protein
Loa Loa (eye worm) LOAG_16060   hypothetical protein
Loa Loa (eye worm) LOAG_13852   hypothetical protein
Loa Loa (eye worm) LOAG_15692   hypothetical protein
Loa Loa (eye worm) LOAG_15566   hypothetical protein
Loa Loa (eye worm) LOAG_15968   hypothetical protein
Mus musculus ENSMUSG00000049488   transmembrane protein 67
Schistosoma japonicum Sjp_0106500   Meckelin, putative
Schistosoma japonicum Sjp_0132950   Meckelin, putative
Schistosoma mansoni Smp_156860   hypothetical protein
Schmidtea mediterranea mk4.000507.11   Meckelin
Schmidtea mediterranea mk4.000507.12   Meckelin
Schmidtea mediterranea mk4.000507.05   Meckelin
Trypanosoma brucei Tb927.5.3540   Meckelin (Transmembrane protein 67), putative
Trypanosoma congolense TcIL3000_5_3970   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511649.110   Meckelin (Transmembrane protein 67), putative
Trypanosoma cruzi TcCLB.507811.90   Meckelin (Transmembrane protein 67), putative
Trichomonas vaginalis TVAG_028900   conserved hypothetical protein
Trichomonas vaginalis TVAG_312380   Meckelin, putative
Trichomonas vaginalis TVAG_384150   conserved hypothetical protein
Trichomonas vaginalis TVAG_016950   conserved hypothetical protein
Trichomonas vaginalis TVAG_185680   conserved hypothetical protein
Trichomonas vaginalis TVAG_178830   conserved hypothetical protein

Essentiality

LmjF.27.0020 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.3540 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.3540 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.3540 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.3540 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.27.0020 (Leishmania major), hypothetical protein, conserved
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