Detailed view for PF3D7_1406500

Basic information

TDR Targets ID: 2395
Plasmodium falciparum, WD repeat-containing protein 65, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.0131 | Length (AA): 1527 | MW (Da): 181534 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 19 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
45 649 1nr0 (A) 37 611 11.00 0.00000000062 0.85 0.44 0.26
353 647 1gxr (A) 463 768 13.00 0.0000034 0.65 0.32 -0.43
479 808 1vyh (C) 92 408 15.00 0 1 0.36 -0.39
489 808 1got (B) 49 340 12.00 0.0000000001 0.98 0.26 -0.49
534 821 1nex (B) 380 671 14.00 0.00000045 0.51 0.22 -0.04
3 147 4ci8 (A) 208 359 19.00 0.000000003 0.42 0.150057 0.62
20 325 4ci8 (A) 226 545 16.00 0.12 1 0.181493 0.33
22 142 4j87 (A) 51 164 12.00 0.00065 0.39 0.0893403 0.14
213 1093 4x2u (A) 200 1084 17.00 0.16 0.86 0.513048 1.04
1191 1376 4uos (A) 1 183 22.00 0.023 0.54 0.446907 -1.57
1457 1521 3iv1 (A) 240 304 18.00 0.2 0 0.358667 -1.81
2 233 2pbi (B) 131 352 9.00 0.026 0.09 0.0892312 0.7
20 246 4ci8 (A) 226 457 16.00 0.16 1 0.186744 0.2
22 157 1erj (A) 483 633 15.00 0.000039 0.48 0.143115 0.32
923 1076 4o1n (A) 14 202 32.00 0.029 0.32 0.145649 0.37
967 1314 4u09 (A) 42 404 30.00 0.00039 0.72 0.233197 1.47
1017 1467 1j7n (A) 27 536 22.00 0.19 0.94 0.316915 1.23
1195 1376 4uos (A) 5 183 29.00 0.12 0.95 0.486058 -1.12
1470 1521 1x8y (A) 313 366 13.00 0.6 0.01 0.280888 -1.88

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile intra-erythrocytic - 32 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Male Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Oocyst, Ring, Sporozoite, Female Gametocyte. Otto TD Zanghi G Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_129382)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG4329   CG4329 gene product from transcript CG4329-RA
Echinococcus granulosus EgrG_000887100   WD repeat containing protein 65
Echinococcus multilocularis EmuJ_000887100   WD repeat containing protein 65
Giardia lamblia GL50803_16840   Trichohyalin
Homo sapiens ENSG00000243710   cilia and flagella associated protein 57
Leishmania braziliensis LbrM.16.1610   hypothetical protein, conserved
Leishmania donovani LdBPK_161650.1   hypothetical protein, conserved
Leishmania infantum LinJ.16.1650   hypothetical protein, conserved
Leishmania major LmjF.16.1550   hypothetical protein, conserved
Leishmania mexicana LmxM.16.1550   hypothetical protein, conserved
Mus musculus ENSMUSG00000028730   cilia and flagella associated protein 57
Neospora caninum NCLIV_040050   WD domain, G-beta repeat-containing protein, putative
Plasmodium berghei PBANKA_1035700   WD repeat-containing protein 65, putative
Plasmodium falciparum PF3D7_1406500   WD repeat-containing protein 65, putative
Plasmodium knowlesi PKNH_1351600   WD repeat-containing protein 65, putative
Plasmodium vivax PVX_086105   hypothetical protein, conserved
Plasmodium yoelii PY07045   hypothetical protein
Schistosoma japonicum Sjp_0131360   IPR009053,Prefoldin,domain-containing
Schistosoma japonicum Sjp_0073520   WD repeat-containing protein 65, putative
Schistosoma japonicum Sjp_0109360   Conserved hypothetical protein
Schistosoma mansoni Smp_062970   hypothetical protein
Schmidtea mediterranea mk4.011754.03  
Schmidtea mediterranea mk4.005200.01  
Schmidtea mediterranea mk4.000067.18  
Schmidtea mediterranea mk4.014204.00  
Schmidtea mediterranea mk4.036055.00  
Schmidtea mediterranea mk4.033970.00  
Schmidtea mediterranea mk4.006017.00  
Schmidtea mediterranea mk4.017690.00  
Trypanosoma brucei gambiense Tbg972.8.4680   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.4870   Component of motile flagella 6
Trypanosoma congolense TcIL3000_0_02340   DIGIT
Trypanosoma cruzi TcCLB.508837.170   hypothetical protein, conserved
Toxoplasma gondii TGME49_265205   WD domain, G-beta repeat-containing protein
Trichomonas vaginalis TVAG_214090   myosin heavy chain, putative

Essentiality

PF3D7_1406500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.4870 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.4870 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.4870 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.4870 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_265205 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_1406500 (Plasmodium falciparum), WD repeat-containing protein 65, putative
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