Detailed view for LmjF.35.1250

Basic information

TDR Targets ID: 23963
Leishmania major, thioredoxin-like protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.7137 | Length (AA): 228 | MW (Da): 25469 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00085   Thioredoxin
PF00462   Glutaredoxin

Gene Ontology

Mouse over links to read term descriptions.
GO:0015035   protein disulfide oxidoreductase activity  
GO:0009055   electron carrier activity  
GO:0045454   cell redox homeostasis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 113 1erv () 4 104 33.00 0.0000000043 1 0.93 -1.15
127 227 1yka (A) 1 101 34.00 0 1 1.02 -1.47
1 82 2av4 (A) 23 103 19.00 0 0.99 0.712149 -0.92
3 119 2diy (A) 22 130 29.00 0.0007 1 0.879958 -0.57
130 227 3zyw (A) 133 230 41.00 0 1 1.13132 -2.08

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127633)

Species Accession Gene Product
Arabidopsis thaliana AT4G04950   monothiol glutaredoxin-S17
Babesia bovis BBOV_II007430   thioredoxin-like protein 2
Babesia bovis BBOV_II007360   Thioredoxin-like protein 2
Brugia malayi Bm1_51225   glutaredoxin-related protein
Candida albicans CaO19.10241   potential thioredoxin similar to S. cerevisiae GRX3 (YDR098C) glutaredoxin
Candida albicans CaO19.2727   potential thioredoxin similar to S. cerevisiae GRX3 (YDR098C) glutaredoxin
Caenorhabditis elegans CELE_D2063.3   Protein GLRX-3, isoform A
Cryptosporidium hominis Chro.60457   thioredoxin-like protein
Cryptosporidium parvum cgd6_3970   glutaredoxin-like protein; 2 thioredoxin folds
Dictyostelium discoideum DDB_G0275555   glutaredoxin family protein
Drosophila melanogaster Dmel_CG6523   CG6523 gene product from transcript CG6523-RA
Echinococcus granulosus EgrG_000195300   glutaredoxin 3
Echinococcus multilocularis EmuJ_000195300   glutaredoxin 3
Homo sapiens ENSG00000108010   glutaredoxin 3
Leishmania braziliensis LbrM.34.1170   thioredoxin-like protein
Leishmania braziliensis LbrM.05.0310   glutaredoxin-like protein
Leishmania donovani LdBPK_050320.1   glutaredoxin-like protein
Leishmania donovani LdBPK_351260.1   thioredoxin-like protein
Leishmania infantum LinJ.05.0320   glutaredoxin-like protein
Leishmania infantum LinJ.35.1260   thioredoxin-like protein
Leishmania major LmjF.05.0310   glutaredoxin-like protein
Leishmania major LmjF.35.1250   thioredoxin-like protein
Leishmania mexicana LmxM.05.0310   glutaredoxin-like protein
Leishmania mexicana LmxM.34.1250   thioredoxin-like protein
Loa Loa (eye worm) LOAG_00884   hypothetical protein
Mus musculus ENSMUSG00000031068   glutaredoxin 3
Neospora caninum NCLIV_015460   hypothetical protein
Oryza sativa 4349034   Os10g0500700
Plasmodium berghei PBANKA_0105500   glutaredoxin-like protein
Plasmodium falciparum PF3D7_0606900   glutaredoxin-like protein
Plasmodium knowlesi PKNH_1143400   glutaredoxin-like protein
Plasmodium vivax PVX_113510   glutaredoxin-like protein, putative
Plasmodium yoelii PY00223   glutaredoxin-related protein
Saccharomyces cerevisiae YER174C   monothiol glutaredoxin GRX4
Saccharomyces cerevisiae YDR098C   monothiol glutaredoxin GRX3
Schistosoma japonicum Sjp_0038240   ko:K07390 monothiol glutaredoxin, putative
Schistosoma mansoni Smp_006550.2   glutaredoxin grx
Schmidtea mediterranea mk4.000927.01   Glutaredoxin-3
Trypanosoma brucei gambiense Tbg972.10.12710   monothiol glutaredoxin, putative
Trypanosoma brucei gambiense Tbg972.5.1310   thioredoxin-like protein
Trypanosoma brucei Tb927.10.10420   monothiol glutaredoxin, putative
Trypanosoma brucei Tb927.5.950   monothiol glutaredoxin, putative
Trypanosoma congolense TcIL3000_0_47240   monothiol glutaredoxin, putative
Trypanosoma congolense TcIL3000_5_680   monothiol glutaredoxin, putative
Trypanosoma cruzi TcCLB.508303.65   thioredoxin-like protein, putative
Trypanosoma cruzi TcCLB.503425.20   monothiol glutaredoxin, putative
Trypanosoma cruzi TcCLB.511179.100   monothiol glutaredoxin, putative
Toxoplasma gondii TGME49_238070   glutaredoxin domain-containing protein
Theileria parva TP02_0196   hypothetical protein

Essentiality

LmjF.35.1250 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.950 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.950 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.950 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.950 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.10420 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.10420 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.10420 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.10420 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0105500 Plasmodium berghei Slow plasmo
TGME49_238070 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.35.1250 (Leishmania major), thioredoxin-like protein
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