Detailed view for Bm1_32440

Basic information

TDR Targets ID: 241614
Brugia malayi, RE01069p
Source Database / ID:  GenBank

pI: 7.2051 | Length (AA): 317 | MW (Da): 36593 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04564   U-box domain
PF12895   Anaphase-promoting complex, cyclosome, subunit 3

Gene Ontology

Mouse over links to read term descriptions.
GO:0000151   ubiquitin ligase complex  
GO:0005488   binding  
GO:0004842   ubiquitin-protein ligase activity  
GO:0005515   protein binding  
GO:0016567   protein ubiquitination  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 103 5hrz (A) 2 100 29.00 0.00002 1 0.820603 -1.3
5 289 2c2l (A) 26 304 45.00 0.000000000018 1 1.37905 0.27
14 76 2q7f (A) 168 230 21.00 0 0.46 0.433138 -0.46
122 275 2f42 (A) 127 270 70.00 0 0.99 1.0191 0.34

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_129825)

Species Accession Gene Product
Arabidopsis thaliana AT3G07370   E3 ubiquitin-protein ligase CHIP
Babesia bovis BBOV_IV004490   tetratricopeptide repeat (TPR)-/ U-box domain-containing protein
Brugia malayi Bm1_32440   RE01069p
Candida albicans CaO19.14154   similar to carboxy terminus of Hsp70-interacting protein
Candida albicans CaO19.6864   similar to carboxy terminus of Hsp70-interacting protein
Caenorhabditis elegans CELE_T09B4.10   Protein CHN-1
Drosophila melanogaster Dmel_CG5203   CG5203 gene product from transcript CG5203-RA
Homo sapiens ENSG00000103266   STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
Loa Loa (eye worm) LOAG_08950   hypothetical protein
Mus musculus ENSMUSG00000039615   STIP1 homology and U-Box containing protein 1
Neospora caninum NCLIV_039890   TPR domain-containing protein, putative
Oryza sativa 4344499   Os08g0113300
Oryza sativa 4337553   Os05g0104900
Plasmodium berghei PBANKA_0804900   E3 ubiquitin-protein ligase, putative
Plasmodium falciparum PF3D7_0707700   E3 ubiquitin-protein ligase, putative
Plasmodium knowlesi PKNH_0106200   E3 ubiquitin-protein ligase, putative
Plasmodium vivax PVX_087910   E3 ubiquitin-protein ligase, putative
Plasmodium yoelii PY00139   ERYTHROCYTE MEMBRANE PROTEIN PFEMP3
Toxoplasma gondii TGME49_265380   tetratricopeptide repeat (TPR)-/ U-box domain-containing protein
Theileria parva TP01_0301   hypothetical protein, conserved
Trichomonas vaginalis TVAG_315350   chaperone binding protein, putative

Essentiality

Bm1_32440 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_T09B4.10 Caenorhabditis elegans larval lethal wormbase
PBANKA_0804900 Plasmodium berghei Slow plasmo
TGME49_265380 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Bm1_32440 (Brugia malayi), RE01069p
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