Detailed view for LmjF.35.5210

Basic information

TDR Targets ID: 24232
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.1327 | Length (AA): 464 | MW (Da): 51585 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01926   50S ribosome-binding GTPase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
57 345 1puj (A) 9 281 25.00 0 1 0.72 -0.59
57 342 1puj (A) 9 278 27.00 0 1 0.9 -0.7
57 342 1puj (A) 9 278 27.00 0 1 0.857279 -0.27
57 343 1puj (A) 9 279 25.00 0 1 0.802534 -0.19
168 237 3iev (A) 8 65 43.00 0.056 0.04 0.128762 0.8
290 458 4uos (A) 3 171 11.00 0.0037 0.08 0.437624 -0.45

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128449)

Species Accession Gene Product
Arabidopsis thaliana AT2G41670   P-loop containing nucleoside triphosphate hydrolases superfamily protein
Babesia bovis BBOV_III011080   hypothetical protein
Brugia malayi Bm1_08080   hypothetical protein
Candida albicans CaO19.2384   similar to S. cerevisiae YMR097C
Candida albicans CaO19.9920   similar to S. cerevisiae YMR097C
Caenorhabditis elegans CELE_Y67D2.4   Protein Y67D2.4, isoform B
Dictyostelium discoideum DDB_G0268990   hypothetical protein
Drosophila melanogaster Dmel_CG17141   CG17141 gene product from transcript CG17141-RA
Echinococcus granulosus EgrG_000147100   mitochondrial GTPase 1
Echinococcus multilocularis EmuJ_000137000   mitochondrial GTPase 1
Echinococcus multilocularis EmuJ_000147100   mitochondrial GTPase 1
Homo sapiens ENSG00000148824   mitochondrial ribosome-associated GTPase 1
Leishmania braziliensis LbrM.34.5160   hypothetical protein, conserved
Leishmania donovani LdBPK_355180.1   hypothetical protein, conserved
Leishmania infantum LinJ.35.5180   hypothetical protein, conserved
Leishmania major LmjF.35.5210   hypothetical protein, conserved
Leishmania mexicana LmxM.34.5210   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_13564   hypothetical protein
Mus musculus ENSMUSG00000039018   mitochondrial GTPase 1 homolog (S. cerevisiae)
Neospora caninum NCLIV_061080   hypothetical protein
Oryza sativa 4350437   Os11g0447300
Plasmodium berghei PBANKA_1008300   GTP-binding protein, putative
Plasmodium falciparum PF3D7_1436500   GTP-binding protein, putative
Plasmodium knowlesi PKNH_0423100   GTP-binding protein, putative
Plasmodium vivax PVX_084710   GTP-binding protein, putative
Plasmodium yoelii PY02303   Drosophila melanogaster CG17141 gene product, putative
Saccharomyces cerevisiae YMR097C   putative GTPase MTG1
Schistosoma japonicum Sjp_0206620   ko:K06948 GTP-binding protein, HSR1-related, putative
Schistosoma mansoni Smp_114160   GTP-binding protein-related
Trypanosoma brucei gambiense Tbg972.9.5090   GTP-binding protein, putative
Trypanosoma brucei Tb927.9.9150   GTP-binding protein, putative
Trypanosoma congolense TcIL3000_9_3230   GTP-binding protein, putative
Trypanosoma cruzi TcCLB.506883.100   GTP-binding protein, putative
Toxoplasma gondii TGME49_219510   GTP binding protein 7 isoform 2 family protein, putative
Theileria parva TP02_0027   hypothetical protein

Essentiality

LmjF.35.5210 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.0810 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.0810 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.0810 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb09.211.0810 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1008300 Plasmodium berghei Essential plasmo
TGME49_219510 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.35.5210 (Leishmania major), hypothetical protein, conserved
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