Detailed view for Bm1_57395

Basic information

TDR Targets ID: 243870
Brugia malayi, splicing factor 3b, subunit 2
Source Database / ID:  GenBank

pI: 8.3532 | Length (AA): 398 | MW (Da): 45751 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04037   Domain of unknown function (DUF382)
PF04046   PSP

Gene Ontology

Mouse over links to read term descriptions.
GO:0005634   nucleus  
GO:0006397   mRNA processing  

Metabolic Pathways

Spliceosome (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 178 4uos (A) 18 186 12.00 0 0.01 0.668123 -1.54
23 134 2fxo (A) 840 958 15.00 0.15 0.01 0.421507 -0.63
101 175 4b1z (M) 425 515 37.00 0.37 0.07 0.345642 0.03
313 372 5lsl (E) 290 346 42.00 0 0.91 0.574254 -0.74

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128039)

Species Accession Gene Product
Arabidopsis thaliana AT4G21660   proline-rich spliceosome-associated (PSP) family protein
Babesia bovis BBOV_IV002140   splicing factor 3B subunit 2
Brugia malayi Bm1_57395   splicing factor 3b, subunit 2
Candida albicans CaO19.7581   similar to C terminus of human spliceosome U2 snRNP protein SAP145 aka SF3B
Caenorhabditis elegans CELE_W03F9.10   Protein W03F9.10
Cryptosporidium hominis Chro.20372   spliceosome associated protein-like
Cryptosporidium parvum cgd2_3510   Cus1p U2 snRNP protein
Dictyostelium discoideum DDB_G0284555   PSP proline-rich domain-containing protein
Drosophila melanogaster Dmel_CG3605   CG3605 gene product from transcript CG3605-RB
Echinococcus granulosus EgrG_000523600   splicing factor 3b subunit 2
Entamoeba histolytica EHI_069550   splicing factor3B subunit 2, putative
Echinococcus multilocularis EmuJ_000523600   splicing factor 3b subunit 2
Giardia lamblia GL50803_17126   Hypothetical protein
Homo sapiens ENSG00000087365   splicing factor 3b, subunit 2, 145kDa
Leishmania braziliensis LbrM.30.0650   spliceosome-associated protein, putative
Leishmania donovani LdBPK_300590.1   spliceosome-associated protein, putative
Leishmania infantum LinJ.30.0590   spliceosome-associated protein, putative
Leishmania major LmjF.30.0570   spliceosome-associated protein, putative
Leishmania mexicana LmxM.29.0570   spliceosome-associated protein, putative
Loa Loa (eye worm) LOAG_01491   splicing factor 3b
Mus musculus ENSMUSG00000024853   splicing factor 3b, subunit 2
Neospora caninum NCLIV_057640   mRNA splicing factor 3bA, related
Oryza sativa 4331222   Os02g0827300
Onchocerca volvulus OVOC7388   Splicing factor 3B subunit 2 homolog
Plasmodium berghei PBANKA_1325300   splicing factor 3B subunit 2, putative
Plasmodium falciparum PF3D7_1461600   splicing factor 3B subunit 2, putative
Plasmodium knowlesi PKNH_1220100   splicing factor 3B subunit 2, putative
Plasmodium vivax PVX_117375   splicing factor 3B subunit 2, putative
Plasmodium yoelii PY04139   Unknown-related
Saccharomyces cerevisiae YMR240C   Cus1p
Schistosoma japonicum Sjp_0007110   Splicing factor 3B subunit 2, putative
Schistosoma mansoni Smp_096810.2   hypothetical protein
Schistosoma mansoni Smp_096810.1   hypothetical protein
Schmidtea mediterranea mk4.000032.09  
Schmidtea mediterranea mk4.000796.03  
Trypanosoma brucei gambiense Tbg972.6.1720   spliceosome-associated protein, putative
Trypanosoma brucei Tb927.6.2000   spliceosome-associated protein, putative
Trypanosoma congolense TcIL3000_6_1580   spliceosome-associated protein, putative
Trypanosoma cruzi TcCLB.504071.50   spliceosome-associated protein, putative
Toxoplasma gondii TGME49_314740   PSP protein
Theileria parva TP03_0436   hypothetical protein, conserved
Trichomonas vaginalis TVAG_081790   cus1 protein, putative

Essentiality

Bm1_57395 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.2000 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.2000 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.2000 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.2000 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_W03F9.10 Caenorhabditis elegans embryonic lethal wormbase
CELE_W03F9.10 Caenorhabditis elegans larval arrest wormbase
CELE_W03F9.10 Caenorhabditis elegans slow growth wormbase
CELE_W03F9.10 Caenorhabditis elegans sterile wormbase
YMR240C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1325300 Plasmodium berghei Slow plasmo
TGME49_314740 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Bm1_57395 (Brugia malayi), splicing factor 3b, subunit 2
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