Detailed view for Bm1_24480

Basic information

TDR Targets ID: 243915
Brugia malayi, DNA replication licensing factor MCM5
Source Database / ID:  GenBank

pI: 8.5615 | Length (AA): 738 | MW (Da): 83120 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00493   MCM2/3/5 family
PF14551   MCM N-terminal domain
PF17207   MCM OB domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0042555   MCM complex  
GO:0005634   nucleus  
GO:0005524   ATP binding  
GO:0003688   DNA replication origin binding  
GO:0003677   DNA binding  
GO:0006270   DNA replication initiation  
GO:0006260   DNA replication  

Metabolic Pathways

DNA replication (KEGG)
Cell cycle (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
32 187 4ywk (A) 2 218 21.00 0 1 0.474882 -0.3
35 301 4pof (A) 5 251 25.00 0 1 0.628289 -0.15
38 657 4r7y (A) 12 1964 39.00 0 1 1.18451 0.48

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127979)

Species Accession Gene Product
Arabidopsis thaliana AT2G07690   minichromosome maintenance protein 5
Babesia bovis BBOV_IV010040   DNA replication licensing factor MCM5, putative
Brugia malayi Bm1_24480   DNA replication licensing factor MCM5
Candida albicans CaO19.5487   part of ARS replication initiation complex
Candida albicans CaO19.12942   part of ARS replication initiation complex
Caenorhabditis elegans CELE_R10E4.4   Protein MCM-5
Cryptosporidium hominis Chro.70329   DNA replication licensing factor mcm5
Cryptosporidium parvum cgd7_2920   DNA replication licensing factor MCM5 like AAA+ ATpase
Dictyostelium discoideum DDB_G0292958   MCM family protein
Drosophila melanogaster Dmel_CG4082   Minichromosome maintenance 5
Entamoeba histolytica EHI_069980   DNA replication licensing factor
Giardia lamblia GL50803_89112   MCM5
Homo sapiens ENSG00000100297   minichromosome maintenance complex component 5
Leishmania braziliensis LbrM.24.0920   minchromosome maintenance (MCM) complex subunit, putative
Leishmania donovani LdBPK_240930.1   minchromosome maintenance (MCM) complex subunit, putative
Leishmania infantum LinJ.24.0930   minchromosome maintenance (MCM) complex subunit, putative
Leishmania major LmjF.24.0910   minchromosome maintenance (MCM) complex subunit, putative
Leishmania mexicana LmxM.24.0910   minchromosome maintenance (MCM) complex subunit, putative
Loa Loa (eye worm) LOAG_11821   DNA replication licensing factor MCM5
Loa Loa (eye worm) LOAG_14825   hypothetical protein
Mus musculus ENSMUSG00000005410   minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae)
Neospora caninum NCLIV_018520   DNA replication licensing factor, putative
Oryza sativa 4331016   Os02g0797400
Plasmodium berghei PBANKA_0610200   DNA replication licensing factor MCM5, putative
Plasmodium falciparum PF3D7_1211700   DNA replication licensing factor MCM5, putative
Plasmodium knowlesi PKNH_1311800   DNA replication licensing factor MCM5, putative
Plasmodium vivax PVX_084615   DNA replication licensing factor MCM5, putative
Plasmodium yoelii PY04668   DNA replication licensing factor MCM5
Saccharomyces cerevisiae YLR274W   MCM DNA helicase complex subunit MCM5
Schistosoma japonicum Sjp_0017340   ko:K02209 minichromosome maintenance protein 5 (cell division control protein, putative
Schistosoma japonicum Sjp_0096310   DNA replication licensing factor mcm5-B, putative
Schistosoma japonicum Sjp_0017350   ko:K02209 minichromosome maintenance protein 5 (cell division control protein, putative
Schistosoma mansoni Smp_143490   DNA replication licensing factor MCM5
Schmidtea mediterranea mk4.039056.00  
Schmidtea mediterranea mk4.003558.01   MCM5
Schmidtea mediterranea mk4.003043.02  
Schmidtea mediterranea mk4.032990.00  
Trypanosoma brucei gambiense Tbg972.11.6280   minichromosome maintenance (MCM) complex subunit, putative,DNA replication licensing factor, putative
Trypanosoma brucei Tb927.11.5570   DNA replication licensing factor MCM5
Trypanosoma cruzi TcCLB.508647.140   DNA replication licensing factor MCM5, putative
Toxoplasma gondii TGME49_243920   DNA replication licensing factor MCM5, putative
Theileria parva TP01_0722   DNA replication licensing factor MCM5, putative
Trichomonas vaginalis TVAG_484360   DNA replication licensing factor MCM5, putative

Essentiality

Bm1_24480 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.3270 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.3270 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.3270 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.3270 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_R10E4.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_R10E4.4 Caenorhabditis elegans sterile wormbase
YLR274W Saccharomyces cerevisiae inviable yeastgenome
TGME49_243920 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Bm1_24480 (Brugia malayi), DNA replication licensing factor MCM5
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