Detailed view for LmjF.34.1060

Basic information

TDR Targets ID: 24451
Leishmania major, mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.3717 | Length (AA): 623 | MW (Da): 69186 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)
PF01434   Peptidase family M41

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0017111   nucleoside-triphosphatase activity  
GO:0008270   zinc ion binding  
GO:0005524   ATP binding  
GO:0004222   metalloendopeptidase activity  
GO:0000166   nucleotide binding  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 623 1ypw (A) 29 738 17.00 0 1 0.83 0.78
165 418 1lv7 (A) 147 399 50.00 0 1 1.08 -1.24
173 616 2dhr (A) 156 600 43.00 0 1 1.23 -0.32
159 616 2dhr (A) 143 600 41.00 0 1 1.18895 0.19
166 420 2qz4 (A) 304 558 57.00 0 1 0.98511 -0.53
340 419 4a3v (B) 380 455 26.00 0 0.93 0.486311 -0.89
344 413 2dzn (D) 348 413 21.00 0 0.76 0.39326 -0.87

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126616)

Species Accession Gene Product
Arabidopsis thaliana AT1G50250   ATP-dependent zinc metalloprotease FTSH 1
Arabidopsis thaliana AT5G42270   ATP-dependent zinc metalloprotease FTSH 5
Arabidopsis thaliana AT1G06430   ATP-dependent zinc metalloprotease FTSH 8
Arabidopsis thaliana AT5G53170   ATP-dependent zinc metalloprotease FTSH 11
Arabidopsis thaliana AT1G07510   FTSH protease 10
Arabidopsis thaliana AT2G26140   cell division protease ftsH-4
Arabidopsis thaliana AT5G15250   ATP-dependent zinc metalloprotease FTSH 6
Arabidopsis thaliana AT2G29080   FTSH protease 3
Arabidopsis thaliana AT2G30950   ATP-dependent zinc metalloprotease FTSH 2
Babesia bovis BBOV_IV011870   cell division protein metalloprotease FtsH, putative
Babesia bovis BBOV_II000870   ATP-dependent metalloprotease FtsH family protein
Babesia bovis BBOV_III005230   ATP-dependent metalloprotease FtsH family protein
Brugia malayi Bm1_51150   ATP-dependent metalloprotease FtsH family protein
Brugia malayi Bm1_49665   Human spg
Brugia malayi Bm1_45815   YME1 protein homolog
Candida albicans CaO19.1252   Mitochondrial member of the AAA family of ATPases
Candida albicans CaO19.8836   Mitochondrial member of the AAA family of ATPases
Candida albicans CaO19.1669   ATP-dependent metalloprotease similar to S. cerevisiae AFG3 (YER017C) and VTA12 (YMR089C)
Candida albicans CaO19.9604   Mitochondrial ATPase
Candida albicans CaO19.9238   ATP-dependent metalloprotease similar to S. cerevisiae AFG3 (YER017C) and VTA12 (YMR089C)
Candida albicans CaO19.2057   Mitochondrial ATPase
Caenorhabditis elegans CELE_Y38F2AR.7   Protein PPGN-1
Caenorhabditis elegans CELE_Y73B3A.21   Protein Y73B3A.21
Caenorhabditis elegans CELE_M03C11.5   Protein YMEL-1
Caenorhabditis elegans CELE_Y47G6A.10   Protein SPG-7
Cryptosporidium parvum cgd1_3360   predicted AFG1 ATpase family AAA ATpase
Chlamydia trachomatis CT_841   ATP-dependent zinc metalloprotease FtsH
Dictyostelium discoideum DDB_G0284249   peptidase M41, FtsH domain-containing protein
Dictyostelium discoideum DDB_G0272120   peptidase M41, FtsH domain-containing protein
Dictyostelium discoideum DDB_G0293388   ATP-dependent metalloprotease
Dictyostelium discoideum DDB_G0267492   hypothetical protein
Drosophila melanogaster Dmel_CG3499   CG3499 gene product from transcript CG3499-RD
Drosophila melanogaster Dmel_CG6512   CG6512 gene product from transcript CG6512-RB
Drosophila melanogaster Dmel_CG2658   CG2658 gene product from transcript CG2658-RC
Escherichia coli b3178   protease, ATP-dependent zinc-metallo
Echinococcus granulosus EgrG_000616300   afg3 protein 2
Echinococcus granulosus EgrG_000927200   paraplegin
Echinococcus granulosus EgrG_000847400   ATP dependent zinc metalloprotease YME1
Echinococcus multilocularis EmuJ_000927200   paraplegin
Echinococcus multilocularis EmuJ_000616300   afg3 protein 2
Echinococcus multilocularis EmuJ_000847400   ATP dependent zinc metalloprotease YME1
Homo sapiens ENSG00000136758   YME1-like 1 ATPase
Homo sapiens ENSG00000141385   AFG3-like AAA ATPase 2
Homo sapiens ENSG00000197912   spastic paraplegia 7 (pure and complicated autosomal recessive)
Leishmania braziliensis LbrM.20.1030   mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Leishmania braziliensis LbrM.35.2900   mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Leishmania donovani LdBPK_362850.1   mitochondrial ATP-dependent zinc metallopeptidase, putative
Leishmania donovani LdBPK_341130.1   mitochondrial ATP-dependent zinc metallopeptidase, putative
Leishmania infantum LinJ.34.1130   mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Leishmania infantum LinJ.36.2850   mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Leishmania major LmjF.36.2710   mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Leishmania major LmjF.34.1060   mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Leishmania mexicana LmxM.36.2710   mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Leishmania mexicana LmxM.33.1060   mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Loa Loa (eye worm) LOAG_07714   hypothetical protein
Loa Loa (eye worm) LOAG_01515   hypothetical protein
Loa Loa (eye worm) LOAG_07954   hypothetical protein
Loa Loa (eye worm) LOAG_09314   spastic paraplegia protein 7 spg-7
Loa Loa (eye worm) LOAG_15756   HflB protein
Loa Loa (eye worm) LOAG_08620   hypothetical protein
Loa Loa (eye worm) LOAG_16099   hypothetical protein
Mycobacterium leprae ML0222   Probable membrane-bound protease FtsH (cell division protein)
Mus musculus ENSMUSG00000031967   AFG3-like AAA ATPase 1
Mus musculus ENSMUSG00000026775   YME1-like 1 (S. cerevisiae)
Mus musculus ENSMUSG00000000738   spastic paraplegia 7 homolog (human)
Mus musculus ENSMUSG00000024527   AFG3-like AAA ATPase 2
Mycobacterium tuberculosis Rv3610c   Membrane-bound protease FtsH (cell division protein)
Mycobacterium ulcerans MUL_4192   membrane-bound protease FtsH
Neospora caninum NCLIV_064680   ATP-dependent metalloprotease involved in cell division, related
Neospora caninum NCLIV_022310   Cell division protease FtsH homolog (EC 3.4.24.- ), related
Oryza sativa 4326311   Os01g0574500
Oryza sativa 4339002   Os05g0458400
Oryza sativa 4327502   Os01g0842600
Oryza sativa 4341796   Os06g0669400
Oryza sativa 4342113   Os06g0725900
Oryza sativa 4326310   Os01g0574400
Oryza sativa 9268843   Os06g0229066
Onchocerca volvulus OVOC6971   Paraplegin homolog
Plasmodium berghei PBANKA_0928500   ATP-dependent zinc metalloprotease FTSH, putative
Plasmodium berghei PBANKA_1328300   ATP-dependent zinc metalloprotease FTSH, putative
Plasmodium berghei PBANKA_1454100   ATP-dependent zinc metalloprotease FTSH 1, putative
Plasmodium falciparum PF3D7_1239700   ATP-dependent zinc metalloprotease FTSH 1
Plasmodium falciparum PF3D7_1464900   ATP-dependent zinc metalloprotease FTSH, putative
Plasmodium falciparum PF3D7_1119600   ATP-dependent zinc metalloprotease FTSH, putative
Plasmodium knowlesi PKNH_1216600   ATP-dependent zinc metalloprotease FTSH, putative
Plasmodium knowlesi PKNH_1459800   ATP-dependent zinc metalloprotease FTSH 1, putative
Plasmodium knowlesi PKNH_0917300   ATP-dependent zinc metalloprotease FTSH, putative
Plasmodium vivax PVX_091615   ATP-dependent zinc metalloprotease FTSH, putative
Plasmodium vivax PVX_117215   ATP-dependent zinc metalloprotease FTSH, putative
Plasmodium vivax PVX_100935   ATP-dependent zinc metalloprotease FTSH 1, putative
Plasmodium yoelii PY04402   ATP-dependent metalloprotease FtsH, putative
Plasmodium yoelii PY05838   afg3-like protein 1
Plasmodium yoelii PY05070   cell division protein ftsh homolog
Saccharomyces cerevisiae YPR024W   i-AAA protease YME1
Saccharomyces cerevisiae YER017C   AAA family ATPase AFG3
Saccharomyces cerevisiae YMR089C   m-AAA protease subunit YTA12
Schistosoma japonicum Sjp_0127130   AFG3-like protein 2, putative
Schistosoma japonicum Sjp_0216800   ko:K09552 spastic paraplegia 7, putative
Schistosoma japonicum Sjp_0090130   ko:K08956 AFG3 family protein, putative
Schistosoma japonicum Sjp_0093270   ko:K01516 nucleoside-triphosphatase [EC3.6.1.15], putative
Schistosoma japonicum Sjp_0308680   AFG3-like protein 2, putative
Schistosoma mansoni Smp_165550   Afg3-like protein 2 (M41 family)
Schistosoma mansoni Smp_055760.2   family M41 unassigned peptidase (M41 family)
Schistosoma mansoni Smp_018620   paraplegin (M41 family)
Schmidtea mediterranea mk4.012027.01   Afg3-like protein 2
Schmidtea mediterranea mk4.005070.02  
Schmidtea mediterranea mk4.005070.03   ATP-dependent zinc metalloprotease YME1 homolog
Schmidtea mediterranea mk4.000889.00   ATP-dependent zinc metalloprotease YME1 homolog
Schmidtea mediterranea mk4.001547.05   ATP-dependent zinc metalloprotease YME1 homolog
Schmidtea mediterranea mk4.000365.08  
Schmidtea mediterranea mk4.000840.09   Paraplegin
Trypanosoma brucei gambiense Tbg972.10.9320   mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E) Family M41
Trypanosoma brucei gambiense Tbg972.4.3310   mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E) Family M41
Trypanosoma brucei Tb927.4.3300   mitochondrial ATP-dependent zinc metallopeptidase, putative
Trypanosoma brucei Tb927.10.7620   mitochondrial ATP-dependent zinc metallopeptidase, putative
Trypanosoma congolense TcIL3000_4_2990   mitochondrial ATP-dependent zinc metallopeptidase, putative
Trypanosoma cruzi TcCLB.510105.210   metallo-peptidase, Clan MA(E) Family M41
Trypanosoma cruzi TcCLB.509717.60   mitochondrial ATP-dependent zinc metallopeptidase, putative
Trypanosoma cruzi TcCLB.506435.390   mitochondrial ATP-dependent zinc metallopeptidase, putative
Trypanosoma cruzi TcCLB.507739.20   mitochondrial ATP-dependent zinc metallopeptidase, putative
Toxoplasma gondii TGME49_202630   ATP-dependent metallopeptidase HflB subfamily protein
Toxoplasma gondii TGME49_300020   ATP-dependent metallopeptidase HflB subfamily protein
Treponema pallidum TP0765   cell division protein (ftsH)
Theileria parva TP04_0494   hypothetical protein, conserved
Theileria parva TP01_1122   cell division protein FtsH, putative
Trichomonas vaginalis TVAG_243780   Clan MA, family M41, FtsH endopeptidase-like metallopeptidase
Wolbachia endosymbiont of Brugia malayi Wbm0150   ATP-dependent Zn protease, HflB

Essentiality

LmjF.34.1060 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu3672 Mycobacterium tuberculosis essential nmpdr
Tb927.4.3300 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.3300 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.3300 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.3300 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.7620 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.7620 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.7620 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.7620 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3178 Escherichia coli essential goodall
CELE_Y47G6A.10 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y47G6A.10 Caenorhabditis elegans larval arrest wormbase
CELE_Y47G6A.10 Caenorhabditis elegans slow growth wormbase
CELE_Y47G6A.10 Caenorhabditis elegans sterile wormbase
PBANKA_0928500 Plasmodium berghei Essential plasmo
TGME49_300020 Toxoplasma gondii Probably essential sidik
TGME49_202630 Toxoplasma gondii Probably essential sidik
TGME49_300020 Toxoplasma gondii Probably non-essential sidik
TGME49_202630 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.34.1060 (Leishmania major), mitochondrial ATP-dependent zinc metallopeptidase, putative,metallo-peptidase, Clan MA(E), Family M41
Title for this comment
Comment