Detailed view for LmjF.35.0600

Basic information

TDR Targets ID: 24729
Leishmania major, 60S ribosomal protein L18a, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.9165 | Length (AA): 179 | MW (Da): 20807 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01775   Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
17 132 5cqc (A) 186 319 28.00 0 0.02 0.547545 1.75
70 145 2jxt (A) 2 76 15.00 0 0.05 0.540081 -0.01

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127130)

Species Accession Gene Product
Arabidopsis thaliana AT3G14600   60S ribosomal protein L18a-3
Arabidopsis thaliana AT1G29970   60S ribosomal protein L18a-1
Arabidopsis thaliana AT2G34480   60S ribosomal protein L18a-2
Arabidopsis thaliana AT1G29965   ribosomal protein L18ae/LX family protein
Babesia bovis BBOV_III004630   60S ribosomal protein L18ae, putative
Brugia malayi Bm1_23030   60S ribosomal protein L18a
Candida albicans CaO19.12102   likely cytosolic ribosomal protein similar to S. cerevisiae RPL20B (YOR312C) large subunit protein L20 (L18)
Candida albicans CaO19.4632   likely cytosolic ribosomal protein similar to S. cerevisiae RPL20B (YOR312C) large subunit protein L20 (L18)
Cryptosporidium hominis Chro.70045   60S ribosomal protein L18a
Cryptosporidium parvum cgd7_320   putative 60S ribosomal protein L18A
Dictyostelium discoideum DDB_G0285881   S60 ribosomal protein L18a
Drosophila melanogaster Dmel_CG6510   Ribosomal protein L18A
Echinococcus granulosus EgrG_000581100   ribosomal protein L18a
Entamoeba histolytica EHI_163670   ribosomal protein L18a, putative
Entamoeba histolytica EHI_035600   ribosomal protein L18a, putative
Entamoeba histolytica EHI_090020   ribosomal protein L18a, putative
Entamoeba histolytica EHI_131940   ribosomal protein L18a, putative
Echinococcus multilocularis EmuJ_000581100   ribosomal protein L18a
Giardia lamblia GL50803_16387   Ribosomal protein L18a
Homo sapiens ENSG00000105640   ribosomal protein L18a
Leishmania braziliensis LbrM.34.0600   60S ribosomal protein L18a, putative
Leishmania donovani LdBPK_350600.1   60S ribosomal protein L18a, putative
Leishmania infantum LinJ.32.0930   60S ribosomal protein L18a, putative
Leishmania infantum LinJ.35.0600   60S ribosomal protein L18a, putative
Leishmania major LmjF.32.0880   60S ribosomal protein L18a, putative
Leishmania major LmjF.35.0600   60S ribosomal protein L18a, putative
Leishmania mexicana LmxM.31.0880   60S ribosomal protein L18a, putative
Leishmania mexicana LmxM.34.0600   60S ribosomal protein L18a, putative
Loa Loa (eye worm) LOAG_10387   60S ribosomal protein L18a
Mus musculus ENSMUSG00000045128   ribosomal protein L18A
Neospora caninum NCLIV_025190   LOC549444 protein, related
Oryza sativa 4327004   Os01g0752300
Oryza sativa 4326865   Os01g0666900
Oryza sativa 4339625   Os05g0565000
Plasmodium berghei PBANKA_1354400   60S ribosomal protein L18, putative
Plasmodium falciparum PF3D7_1341200   60S ribosomal protein L18, putative
Plasmodium knowlesi PKNH_1260200   60S ribosomal subunit protein L18, putative
Plasmodium vivax PVX_082965   60S ribosomal protein L18a, putative
Plasmodium yoelii PY01391   Ribosomal L18ae protein family
Saccharomyces cerevisiae YMR242C   ribosomal 60S subunit protein L20A
Saccharomyces cerevisiae YOR312C   ribosomal 60S subunit protein L20B
Schistosoma japonicum Sjp_0201600   ko:K02882 large subunit ribosomal protein L18Ae, putative
Schistosoma mansoni Smp_185810   60S ribosomal protein L18a
Schistosoma mansoni Smp_191540   60S ribosomal protein L18a
Schmidtea mediterranea mk4.001900.07   60S ribosomal protein L18a
Trypanosoma brucei gambiense Tbg972.10.4760   60S ribosomal protein L18a, putative
Trypanosoma brucei gambiense Tbg972.11.15790   ribosomal protein L18, putative
Trypanosoma brucei Tb927.11.14130   ribosomal protein L18, putative
Trypanosoma brucei Tb927.10.3840   60S ribosomal protein L18a, putative
Trypanosoma brucei Tb427tmp.01.5720   ribosomal protein L18, putative
Trypanosoma congolense TcIL3000_10_3180   ribosomal protein L18, putative
Trypanosoma congolense TcIL3000.11.14430   ribosomal protein L18, putative
Trypanosoma cruzi TcCLB.511727.129   ribosomal protein L18, putative
Trypanosoma cruzi TcCLB.506957.90   60S ribosomal protein L18a, putative
Trypanosoma cruzi TcCLB.511001.120   60S ribosomal protein L18a, putative
Toxoplasma gondii TGME49_262670   ribosomal protein RPL18A
Theileria parva TP02_0803   60S ribosomal protein L18, putative
Trichomonas vaginalis TVAG_347250   60S ribosomal protein L18a, putative
Trichomonas vaginalis TVAG_416010   60S ribosomal protein L18a, putative
Trichomonas vaginalis TVAG_263760   60S ribosomal protein L18a, putative
Trichomonas vaginalis TVAG_061890   60S ribosomal protein L18a, putative

Essentiality

LmjF.35.0600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.5720 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.5720 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.5720 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.5720 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.3840 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.3840 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.3840 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.3840 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1354400 Plasmodium berghei Essential plasmo
TGME49_262670 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.35.0600 (Leishmania major), 60S ribosomal protein L18a, putative
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