Detailed view for PF3D7_1414600

Basic information

TDR Targets ID: 2477
Plasmodium falciparum, mRNA-capping enzyme subunit alpha
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.3339 | Length (AA): 520 | MW (Da): 61154 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01331   mRNA capping enzyme, catalytic domain
PF03919   mRNA capping enzyme, C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0004484   mRNA guanylyltransferase activity  
GO:0006397   mRNA processing  
GO:0006370   mRNA capping  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
16 239 1ckm (A) 34 236 24.00 0.00000038 1 0.62 -0.32
1 519 1p16 (A) 1 380 27.00 0 1 0.615177 1.81
10 215 3rtx (A) 242 463 23.00 0 0.81 0.589854 0.15
331 519 4pz7 (A) 189 366 34.00 0.000000000044 0.6 0.478062 1.06

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, early ring, late ring, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, merozoite, sporozoite, early trophozoite, late schizont, late trophozoite, Male Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile early schizont, Oocyst, Sporozoite, Female Gametocyte. PlasmoDB Zanghi G Lasonder E
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_128005)

Species Accession Gene Product
Arabidopsis thaliana AT5G28210   mRNA capping enzyme family protein
Arabidopsis thaliana AT3G09100   mRNA capping enzyme family protein
Arabidopsis thaliana AT5G01290   mRNA capping enzyme family protein
Babesia bovis BBOV_III006110   mRNA capping enzyme, C-terminal domain containing protein
Brugia malayi Bm1_12210   mRNA capping enzyme, C-terminal domain containing protein
Candida albicans CaO19.1261   mRNA guanylyltransferase/mRNA capping enzyme
Candida albicans CaO19.8846   mRNA guanylyltransferase/mRNA capping enzyme
Caenorhabditis elegans CELE_C03D6.3   Protein CEL-1, isoform B
Cryptosporidium hominis Chro.60277   mRNA capping enzyme alpha subunit
Cryptosporidium parvum cgd6_2380   mRNA capping enzyme; RNA guanylyltransferase Ceg1p
Dictyostelium discoideum DDB_G0289199   hypothetical protein
Drosophila melanogaster Dmel_CG1810   mRNA-capping-enzyme
Echinococcus granulosus EgrG_000208300   mrna capping enzyme
Entamoeba histolytica EHI_109940   mRNA capping enzyme, putative
Echinococcus multilocularis EmuJ_000208300   mrna capping enzyme
Giardia lamblia GL50803_17308   mRNA capping enzyme alpha subunit
Homo sapiens ENSG00000111880   RNA guanylyltransferase and 5'-phosphatase
Loa Loa (eye worm) LOAG_08948   hypothetical protein
Mus musculus ENSMUSG00000028274   RNA guanylyltransferase and 5'-phosphatase
Neospora caninum NCLIV_043900   AT3g09100/MZB10_13, related
Oryza sativa 4350086   Os11g0217500
Oryza sativa 4351709   Os12g0193200
Plasmodium berghei PBANKA_1028100   mRNA-capping enzyme subunit alpha, putative
Plasmodium falciparum PF3D7_1414600   mRNA-capping enzyme subunit alpha
Plasmodium knowlesi PKNH_1343500   mRNA-capping enzyme subunit alpha, putative
Plasmodium vivax PVX_085720   mRNA capping enzyme, putative
Plasmodium yoelii PY05095   mRNA capping enzyme, putative
Saccharomyces cerevisiae YGL130W   Ceg1p
Schistosoma japonicum Sjp_0214610   mRNA-capping enzyme, putative
Schistosoma japonicum Sjp_0301500   ko:K00987 mRNA guanylyltransferase [EC2.7.7.50], putative
Schistosoma mansoni Smp_132720.2   mRNA-capping enzyme
Schmidtea mediterranea mk4.001408.03  
Schmidtea mediterranea mk4.000973.00   mRNA-capping enzyme
Schmidtea mediterranea mk4.012610.01  
Schmidtea mediterranea mk4.006910.02  
Schmidtea mediterranea mk4.006910.03   mRNA-capping enzyme
Schmidtea mediterranea mk4.000973.02   mRNA-capping enzyme
Schmidtea mediterranea mk4.003218.01   mRNA-capping enzyme
Schmidtea mediterranea mk4.017652.00  
Schmidtea mediterranea mk4.021873.01  
Schmidtea mediterranea mk4.001408.02   mRNA-capping enzyme
Schmidtea mediterranea mk4.006903.00   mRNA-capping enzyme
Schmidtea mediterranea mk4.001439.01   mRNA-capping enzyme
Toxoplasma gondii TGME49_305320   mRNA capping enzyme
Theileria parva TP02_0352   mRNA capping enzyme, putative
Trichomonas vaginalis TVAG_187730   mRNA capping enzyme, putative

Essentiality

PF3D7_1414600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_C03D6.3 Caenorhabditis elegans embryonic arrest wormbase
CELE_C03D6.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_C03D6.3 Caenorhabditis elegans larval arrest wormbase
CELE_C03D6.3 Caenorhabditis elegans slow growth wormbase
YGL130W Saccharomyces cerevisiae inviable yeastgenome
TGME49_305320 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1414600 (Plasmodium falciparum), mRNA-capping enzyme subunit alpha
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