Detailed view for LmjF.34.4000

Basic information

TDR Targets ID: 24817
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.543 | Length (AA): 506 | MW (Da): 56254 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07910   Peptidase family C78

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
52 499 3oqc (A) 33 456 32.00 0 1 0.961176 0.98
58 499 3oqc (A) 15 456 33.00 0 1 1.01632 0.78
281 500 2z84 (A) 10 216 36.00 0 1 0.869583 -0.61

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_129023)

Species Accession Gene Product
Arabidopsis thaliana AT3G48380   putative Ufm1-specific protease
Babesia bovis BBOV_III009030   protein of unknown function (DUF1671) protein family
Brugia malayi Bm1_39095   hypothetical protein
Caenorhabditis elegans CELE_F38A5.1   Protein F38A5.1, isoform B
Cryptosporidium hominis Chro.60116   CG16979 protein
Cryptosporidium parvum cgd6_900   SPAC25H1.04/CG16979-cterm -like; cysteine protease
Dictyostelium discoideum DDB_G0282953   hypothetical protein
Drosophila melanogaster Dmel_CG16979   CG16979 gene product from transcript CG16979-RA
Drosophila melanogaster Dmel_CG30157   CG30157 gene product from transcript CG30157-RA
Echinococcus granulosus EgrG_000627900   E3 ubiquitin protein ligase listerin
Echinococcus granulosus EgrG_000799700   ufm1 specific protease 1
Echinococcus granulosus EgrG_000586500   Ufm1 specific protease 2
Echinococcus multilocularis EmuJ_000799700   ufm1 specific protease 1
Echinococcus multilocularis EmuJ_000627900   E3 ubiquitin protein ligase listerin
Echinococcus multilocularis EmuJ_000586500   Ufm1 specific protease 2
Homo sapiens 55325   UFM1-specific peptidase 2
Homo sapiens ENSG00000176125   UFM1-specific peptidase 1 (non-functional)
Leishmania braziliensis LbrM.20.3630   hypothetical protein, conserved
Leishmania donovani LdBPK_343830.1   Peptidase family C78, putative
Leishmania infantum LinJ.34.3830   hypothetical protein, conserved
Leishmania major LmjF.34.4000   hypothetical protein, conserved
Leishmania mexicana LmxM.33.4000   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_03930   Ufm1-specific protease 2
Mus musculus ENSMUSG00000051502   UFM1-specific peptidase 1
Mus musculus ENSMUSG00000031634   UFM1-specific peptidase 2
Neospora caninum NCLIV_060120   hypothetical protein, conserved
Oryza sativa 4351994   Os12g0285500
Schistosoma japonicum Sjp_0213530   expressed protein
Schistosoma japonicum Sjp_0082310   Probable Ufm1-specific protease 2, putative
Schistosoma japonicum Sjp_0213520   Zinc finger protein 294, putative
Schistosoma japonicum Sjp_0213540   expressed protein
Schistosoma japonicum Sjp_0308760   IPR012462,Peptidase, ubiquitin fold modifier-specific,domain-containing
Schistosoma japonicum Sjp_0215670   ko:K01376 Peptidase, ubiquitin fold modifier-specific [EC:3.4.22.-], putative
Schistosoma japonicum Sjp_0317800   Ufm1-specific protease 2, putative
Schistosoma japonicum Sjp_0116050   expressed protein
Schistosoma mansoni Smp_179040   UfSP2 peptidase (C78 family)
Schmidtea mediterranea mk4.006398.01   Probable Ufm1-specific protease
Trypanosoma brucei gambiense Tbg972.4.500   hypothetical protein, conserved
Trypanosoma brucei Tb927.4.730   Peptidase family C78, putative
Trypanosoma congolense TcIL3000_0_00270   Peptidase family C78, putative
Trypanosoma cruzi TcCLB.507053.30   Peptidase family C78, putative
Toxoplasma gondii TGME49_216020   peptidase family c78 protein
Theileria parva TP04_0807   hypothetical protein

Essentiality

LmjF.34.4000 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.730 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.730 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.730 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.730 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_216020 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.34.4000 (Leishmania major), hypothetical protein, conserved
Title for this comment
Comment