Detailed view for LmjF.34.2470

Basic information

TDR Targets ID: 25083
Leishmania major, ribosomal protein l35a, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 12.2751 | Length (AA): 144 | MW (Da): 16454 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01247   Ribosomal protein L35Ae

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
33 141 2lp6 (A) 1 90 28.00 0 0.85 0.898344 -0.01
35 139 2lp6 (A) 3 88 33.00 0.0000054 0.66 0.913667 -0.16

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127072)

Species Accession Gene Product
Arabidopsis thaliana AT1G07070   60S ribosomal protein L35a-1
Arabidopsis thaliana AT1G74270   60S ribosomal protein L35a-3
Arabidopsis thaliana AT3G55750   60S ribosomal protein L35a-4
Arabidopsis thaliana AT1G41880   60S ribosomal protein L35a-2
Babesia bovis BBOV_III001970   60S ribosomal protein L35a, putative
Brugia malayi Bm1_38825   60S ribosomal protein L35a
Brugia malayi Bm1_52910   60S ribosomal protein L35a
Caenorhabditis elegans CELE_F10E7.7   Protein RPL-33
Cryptosporidium hominis Chro.70290   60S ribosomal protein L35a (RPL35aC)
Cryptosporidium parvum cgd7_2540   60S ribosomal protein L35A
Dictyostelium discoideum DDB_G0270424   S60 ribosomal protein L35a
Drosophila melanogaster Dmel_CG2099   Ribosomal protein L35A
Echinococcus granulosus EgrG_000458500   Ribosomal protein L35A
Entamoeba histolytica EHI_025590   60S ribosomal protein L35a, putative
Entamoeba histolytica EHI_015310   60S ribosomal protein L35a, putative
Entamoeba histolytica EHI_014110   60S ribosomal protein L35a, putative
Entamoeba histolytica EHI_114920   60S ribosomal protein L35a, putative
Entamoeba histolytica EHI_049000   60S ribosomal protein L35a, putative
Echinococcus multilocularis EmuJ_000458500   Ribosomal protein L35A
Giardia lamblia GL50803_5947   Ribosomal protein L35a
Homo sapiens ENSG00000182899   ribosomal protein L35a
Leishmania braziliensis LbrM.10.0070   ribosomal protein l35a, putative
Leishmania braziliensis LbrM.20.1970   ribosomal protein l35a, putative
Leishmania donovani LdBPK_342240.1   ribosomal protein l35a, putative
Leishmania infantum LinJ.10.0050   ribosomal protein l35a, putative
Leishmania infantum LinJ.34.2240   ribosomal protein l35a, putative
Leishmania major LmjF.10.0070   ribosomal protein l35a, putative
Leishmania major LmjF.34.2470   ribosomal protein l35a, putative
Leishmania mexicana LmxM.10.0070   ribosomal protein l35a, putative
Leishmania mexicana LmxM.33.2470   ribosomal protein l35a, putative
Loa Loa (eye worm) LOAG_00148   60S ribosomal protein L35a
Loa Loa (eye worm) LOAG_06279   60S ribosomal protein L35a
Mus musculus ENSMUSG00000060636   ribosomal protein L35A
Mus musculus 100039532   predicted gene 10029
Neospora caninum NCLIV_065450   hypothetical protein
Oryza sativa 4339572   Os05g0556900
Oryza sativa 4330946   Os02g0785800
Plasmodium berghei PBANKA_0906400   60S ribosomal protein L35ae, putative
Plasmodium falciparum PF3D7_1142600   60S ribosomal protein L35ae, putative
Plasmodium knowlesi PKNH_0940400   60S ribosomal protein L35ae, putative
Plasmodium vivax PVX_092735   60S ribosomal protein L35ae, putative
Plasmodium yoelii PY05736   Ribosomal protein L35Ae, putative
Saccharomyces cerevisiae YOR234C   ribosomal 60S subunit protein L33B
Saccharomyces cerevisiae YPL143W   ribosomal 60S subunit protein L33A
Schistosoma mansoni Smp_061200   60S ribosomal protein L35a
Schmidtea mediterranea mk4.003170.01   60S ribosomal protein L35a
Trypanosoma brucei gambiense Tbg972.4.2090   60S ribosomal protein L35a, putative
Trypanosoma brucei Tb927.4.2180   60S ribosomal protein L35a, putative
Trypanosoma congolense TcIL3000_4_1900   60S ribosomal protein L35a, putative
Trypanosoma cruzi TcCLB.506559.470   ribosomal protein l35a, putative
Toxoplasma gondii TGME49_249250   ribosomal protein RPL35A
Theileria parva TP03_0603   60S ribosomal protein L35a, putative
Trichomonas vaginalis TVAG_114810   60S ribosomal protein L35a, putative
Trichomonas vaginalis TVAG_067370   60S ribosomal protein L35a, putative
Trichomonas vaginalis TVAG_185880   60S ribosomal protein L35a, putative
Trichomonas vaginalis TVAG_192480   60S ribosomal protein L35a, putative

Essentiality

LmjF.34.2470 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.2180 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.2180 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.2180 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.2180 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F10E7.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_F10E7.7 Caenorhabditis elegans larval arrest wormbase
CELE_F10E7.7 Caenorhabditis elegans slow growth wormbase
YPL143W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0906400 Plasmodium berghei Essential plasmo
TGME49_249250 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier LmjF.34.2470 (Leishmania major), ribosomal protein l35a, putative
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