Detailed view for LmjF.36.3210

Basic information

TDR Targets ID: 25217
Leishmania major, 14-3-3 protein-like protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.5192 | Length (AA): 258 | MW (Da): 29688 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00244   14-3-3 protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0019904   protein domain specific binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
26 256 1o9d (A) 5 240 54.00 0 1 1.57 -1.91
28 254 2br9 (A) 3 232 55.00 0 1 1.72 -2.09
26 256 1o9d (A) 5 240 55.00 0 1 1.62885 -1.22
27 254 2npm (A) 26 258 47.00 0 1 1.55422 -1.18
28 254 2br9 (A) 3 232 55.00 0 1 1.64635 -1.35
28 255 2c63 (A) 3 235 50.00 0 1 1.59122 -1.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126706)

Species Accession Gene Product
Arabidopsis thaliana AT5G10450   14-3-3-like protein GF14 lambda
Arabidopsis thaliana AT1G35160   14-3-3-like protein GF14 phi
Arabidopsis thaliana AT5G38480   14-3-3-like protein GF14 psi
Arabidopsis thaliana AT1G26480   14-3-3-like protein GF14 iota
Arabidopsis thaliana AT1G22300   14-3-3-like protein GF14 epsilon
Arabidopsis thaliana AT5G16050   14-3-3-like protein GF14 upsilon
Arabidopsis thaliana AT5G65430   14-3-3-like protein GF14 kappa
Arabidopsis thaliana AT4G09000   14-3-3-like protein GF14 chi
Arabidopsis thaliana AT1G34760   14-3-3-like protein GF14 omicron
Arabidopsis thaliana AT1G78300   14-3-3-like protein GF14 omega
Arabidopsis thaliana AT3G02520   14-3-3-like protein GF14 nu
Babesia bovis BBOV_II006430   14-3-3 protein
Brugia malayi Bm1_10970   14-3-3-like protein 2
Brugia malayi Bm1_18190   14-3-3-like protein 2
Candida albicans CaO19.3014   likely 14-3-3 family protein, similar to S. cerevisiae BMH1 (YER177W) and BMH2 (YDR099W) which is involved in RAS protein signal
Candida albicans CaO19.10532   likely 14-3-3 family protein, similar to S. cerevisiae BMH1 (YER177W) and BMH2 (YDR099W) which is involved in RAS protein signal
Caenorhabditis elegans CELE_F52D10.3   Protein FTT-2, isoform B
Cryptosporidium hominis Chro.30161   14-3-3-like protein B (14-3-3B)
Cryptosporidium parvum cgd3_1290   14-3-3 domain containing protein
Dictyostelium discoideum DDB_G0269138   hypothetical protein
Drosophila melanogaster Dmel_CG31196   CG31196 gene product from transcript CG31196-RD
Drosophila melanogaster Dmel_CG17870   CG17870 gene product from transcript CG17870-RE
Echinococcus granulosus EgrG_001192500   14-3-3 protein
Echinococcus granulosus EgrG_001060100   14-3-3 protein zeta:delta
Echinococcus granulosus EgrG_000364000   14-3-3 protein beta:alpha
Echinococcus granulosus EgrG_000231300   14-3-3 protein epsilon
Echinococcus granulosus EgrG_000314100   14-3-3 protein gamma
Echinococcus granulosus EgrG_000789700   14-3-3 protein
Entamoeba histolytica EHI_098280   14-3-3 protein 2
Entamoeba histolytica EHI_006810   14-3-3 protein 3
Entamoeba histolytica EHI_025360   14-3-3 protein 1
Echinococcus multilocularis EmuJ_000231300   14-3-3 protein epsilon
Echinococcus multilocularis EmuJ_000314100  
Echinococcus multilocularis EmuJ_001192500   14-3-3 protein
Echinococcus multilocularis EmuJ_001060100   14-3-3 protein zeta:delta
Echinococcus multilocularis EmuJ_000364000   14-3-3 protein beta:alpha
Echinococcus multilocularis EmuJ_000789700   14-3-3 protein
Giardia lamblia GL50803_6430   14-3-3 protein
Homo sapiens ENSG00000164924   tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
Homo sapiens ENSG00000166913   tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
Homo sapiens ENSG00000108953   tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
Leishmania braziliensis LbrM.35.3430   14-3-3 protein-like protein
Leishmania braziliensis LbrM.11.0040   14-3-3 protein, putative
Leishmania donovani LdBPK_110350.1   14-3-3 protein 2, putative
Leishmania donovani LdBPK_363360.1   14-3-3 protein 1, putative
Leishmania infantum LinJ.11.0350   14-3-3 protein, putative
Leishmania infantum LinJ.36.3360   14-3-3 protein-like protein
Leishmania major LmjF.36.3210   14-3-3 protein-like protein
Leishmania major LmjF.11.0350   14-3-3 protein, putative
Leishmania mexicana LmxM.11.0350   14-3-3 protein, putative
Leishmania mexicana LmxM.36.3210   14-3-3 protein-like protein
Loa Loa (eye worm) LOAG_05701   hypothetical protein
Mus musculus ENSMUSG00000018326   tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
Mus musculus ENSMUSG00000020849   tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
Mus musculus ENSMUSG00000022285   tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Mus musculus 102641764   14-3-3 protein zeta/delta-like
Neospora caninum NCLIV_024820   14-3-3 protein homolog
Oryza sativa 4345634   Os08g0430500
Oryza sativa 4329775   Os02g0580300
Oryza sativa 4333880   Os03g0710800
Oryza sativa 9269633   Os11g0609600
Oryza sativa 4336066   Os04g0462500
Oryza sativa 4350697   Os11g0546900
Plasmodium berghei PBANKA_0712600   14-3-3 protein
Plasmodium falciparum PF3D7_0818200   14-3-3 protein
Plasmodium knowlesi PKNH_0501800   14-3-3 protein, putative
Plasmodium yoelii PY05990   14-3-3 protein
Plasmodium yoelii PY01841   14-3-3 protein
Saccharomyces cerevisiae YER177W   14-3-3 family protein BMH1
Saccharomyces cerevisiae YDR099W   14-3-3 family protein BMH2
Schistosoma japonicum Sjp_0092590   ko:K06630 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation, putative
Schistosoma japonicum Sjp_0204420   ko:K06630 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation, putative
Schistosoma mansoni Smp_034840.2   hypothetical protein
Schistosoma mansoni Smp_034840.1   hypothetical protein
Schistosoma mansoni Smp_034840.3   hypothetical protein
Schistosoma mansoni Smp_002410   hypothetical protein
Schmidtea mediterranea mk4.000081.04   14-3-3 protein epsilon
Schmidtea mediterranea mk4.002915.00   14-3-3 protein epsilon
Schmidtea mediterranea mk4.016050.00   14-3-3 protein zeta
Schmidtea mediterranea mk4.001714.01   14-3-3 protein zeta
Schmidtea mediterranea mk4.005215.00  
Schmidtea mediterranea mk4.009079.02   14-3-3 protein zeta
Schmidtea mediterranea mk4.007483.00  
Schmidtea mediterranea mk4.000094.06  
Schmidtea mediterranea mk4.007444.00   14-3-3 protein zeta
Schmidtea mediterranea mk4.001319.01   14-3-3 protein zeta
Trypanosoma brucei gambiense Tbg972.11.10700   14-3-3 protein-like protein, putative
Trypanosoma brucei gambiense Tbg972.11.7800   14-3-3 protein-like protein, putative
Trypanosoma brucei Tb927.11.6870   14-3-3 protein 2
Trypanosoma brucei Tb927.11.9530   14-3-3 protein 1
Trypanosoma congolense TcIL3000.11.10000   14-3-3 protein 1, putative
Trypanosoma congolense TcIL3000.11.7440   14-3-3 protein 2, putative
Trypanosoma cruzi TcCLB.506775.80   14-3-3 protein 2, putative
Trypanosoma cruzi TcCLB.511589.130   14-3-3 protein 1, putative
Trypanosoma cruzi TcCLB.511167.90   14-3-3 protein 2, putative
Trypanosoma cruzi TcCLB.508851.180   14-3-3 protein 1, putative
Toxoplasma gondii TGME49_263090   14-3-3 protein
Theileria parva TP02_0607   14-3-3 protein, putative
Trichomonas vaginalis TVAG_462940   14-3-3 protein sigma, gamma, zeta, beta/alpha, putative
Trichomonas vaginalis TVAG_454380   14-3-3 protein sigma, gamma, zeta, beta/alpha, putative
Trichomonas vaginalis TVAG_251260   14-3-3 protein sigma, gamma, zeta, beta/alpha, putative
Trichomonas vaginalis TVAG_469400   14-3-3 protein sigma, gamma, zeta, beta/alpha, putative
Trichomonas vaginalis TVAG_198430   14-3-3 protein sigma, gamma, zeta, beta/alpha, putative

Essentiality

LmjF.36.3210 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.1290 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.1290 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.1290 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.1290 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.02.4700 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.4700 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.4700 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.4700 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F52D10.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_F52D10.3 Caenorhabditis elegans larval arrest wormbase
CELE_F52D10.3 Caenorhabditis elegans slow growth wormbase
CELE_F52D10.3 Caenorhabditis elegans sterile wormbase
PBANKA_0712600 Plasmodium berghei Essential plasmo
TGME49_263090 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0042 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.36.3210 (Leishmania major), 14-3-3 protein-like protein
Title for this comment
Comment