Detailed view for LmjF.36.0570

Basic information

TDR Targets ID: 25223
Leishmania major, Mitochondrial elongation factor G, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6164 | Length (AA): 746 | MW (Da): 84210 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00009   Elongation factor Tu GTP binding domain
PF00679   Elongation factor G C-terminus
PF03764   Elongation factor G, domain IV
PF14492   Elongation Factor G, domain II

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  
GO:0003924   GTPase activity  
GO:0003746   translation elongation factor activity  
GO:0006414   translational elongation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
13 741 1n0u (A) 3 830 21.00 0 1 0.9 0.11
21 731 2bm0 (A) 4 689 40.00 0 1 1.4 -0.37
18 420 5ty0 (A) 2 393 46.00 0 1 0.971214 -0.33
21 744 1n0u (A) 11 833 18.00 0 1 0.957709 0.71
25 731 2bm0 (A) 8 689 40.00 0 1 1.32892 -0.13
643 739 3cb4 (D) 399 497 22.00 0.015 0.28 0.368227 -0.72

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126692)

Species Accession Gene Product
Arabidopsis thaliana AT2G45030   elongation factor EF-G
Arabidopsis thaliana AT1G45332   elongation factor G
Arabidopsis thaliana AT1G62750   elongation factor G
Babesia bovis BBOV_III000360   translation elongation factor G, putative
Babesia bovis BBOV_IV004710   translation elongation factor G (EF-G), putative
Brugia malayi Bm1_50625   Elongation factor G C-terminus family protein
Candida albicans CaO19.6208   Mitochondrial elongation factor G-like protein
Candida albicans CaO19.13589   Mitochondrial elongation factor G-like protein
Candida albicans CaO19.12398   Mitochondrial elongation factor G-like protein
Candida albicans CaO19.4932   Mitochondrial elongation factor G-like protein
Caenorhabditis elegans CELE_Y119D3B.14   Protein Y119D3B.14
Caenorhabditis elegans CELE_F29C12.4   Protein F29C12.4
Chlamydia trachomatis CT_437   elongation factor G
Dictyostelium discoideum DDB_G0267766   mitochondrial translation elongation factor G
Dictyostelium discoideum DDB_G0270482   mitochondrial translation elongation factor G
Drosophila melanogaster Dmel_CG4567   iconoclast
Drosophila melanogaster Dmel_CG31159   Elongation Factor G2
Escherichia coli b3340   protein chain elongation factor EF-G, GTP-binding
Echinococcus granulosus EgrG_000424300   translation elongation factor G
Echinococcus granulosus EgrG_000697600   elongation factor G mitochondrial
Echinococcus granulosus EgrG_000424400   translation elongation factor G
Echinococcus multilocularis EmuJ_000697600   elongation factor G, mitochondrial
Echinococcus multilocularis EmuJ_000424400   translation elongation factor G
Homo sapiens ENSG00000168827   G elongation factor, mitochondrial 1
Homo sapiens ENSG00000164347   G elongation factor, mitochondrial 2
Leishmania braziliensis LbrM.28.2160   elongation factor G2-like protein
Leishmania braziliensis LbrM.35.0680   Mitochondrial elongation factor G, putative
Leishmania donovani LdBPK_360620.1   Elongation factor G1, mitochondrial, putative
Leishmania donovani LdBPK_282090.1   Elongation factor G 2, mitochondrial, putative
Leishmania infantum LinJ.36.0620   Mitochondrial elongation factor G, putative
Leishmania infantum LinJ.28.2090   elongation factor G2-like protein
Leishmania major LmjF.28.1960   elongation factor G2-like protein
Leishmania major LmjF.36.0570   Mitochondrial elongation factor G, putative
Leishmania mexicana LmxM.28.1960   elongation factor G2-like protein
Leishmania mexicana LmxM.36.0570   Mitochondrial elongation factor G, putative
Loa Loa (eye worm) LOAG_03866   hypothetical protein
Loa Loa (eye worm) LOAG_14564   hypothetical protein
Loa Loa (eye worm) LOAG_03914   hypothetical protein
Mycobacterium leprae ML1878c   PROBABLE ELONGATION FACTOR G FUSA1 (EF-G)
Mus musculus 28030   G elongation factor, mitochondrial 1
Mus musculus ENSMUSG00000021666   G elongation factor, mitochondrial 2
Mycobacterium tuberculosis Rv0684   Probable elongation factor G FusA1 (EF-G)
Mycobacterium ulcerans MUL_0765   elongation factor G
Neospora caninum NCLIV_048540   elongation factor G, putative
Neospora caninum NCLIV_026830   Translation elongation factor G, related
Oryza sativa 4336525   Os04g0538100
Oryza sativa 4333268   Os03g0565500
Onchocerca volvulus OVOC13440  
Plasmodium berghei PBANKA_0101200   elongation factor G, putative
Plasmodium berghei PBANKA_1447700   elongation factor G, putative
Plasmodium falciparum PF3D7_0602400   elongation factor G
Plasmodium falciparum PF3D7_1233000   elongation factor G
Plasmodium knowlesi PKNH_1452500   elongation factor G, putative
Plasmodium knowlesi PKNH_1148300   elongation factor G, putative
Plasmodium vivax PVX_100600   elongation factor G, putative
Plasmodium vivax PVX_113285   elongation factor G, putative
Plasmodium yoelii PY04706   translation elongation factor G
Plasmodium yoelii PY01864   elongation factor g-related
Saccharomyces cerevisiae YLR069C   Mef1p
Saccharomyces cerevisiae YJL102W   Mef2p
Schistosoma japonicum Sjp_0122460   expressed protein
Schistosoma japonicum Sjp_0306820   Elongation factor G 2, mitochondrial precursor, putative
Schistosoma japonicum Sjp_0037430   Elongation factor G 1, mitochondrial precursor, putative
Schistosoma japonicum Sjp_0057090   Elongation factor G, putative
Schistosoma japonicum Sjp_0080540   ko:K02355 elongation factor EF-G, putative
Schistosoma mansoni Smp_075690   translation elongation factor G
Schistosoma mansoni Smp_165840.2   translation elongation factor G
Schistosoma mansoni Smp_165840.1   translation elongation factor G
Schmidtea mediterranea mk4.034240.00  
Schmidtea mediterranea mk4.012015.01   Ribosome-releasing factor 2, mitochondrial
Schmidtea mediterranea mk4.000296.04   Ribosome-releasing factor 2, mitochondrial
Schmidtea mediterranea mk4.001580.04  
Schmidtea mediterranea mk4.001580.03  
Schmidtea mediterranea mk4.016148.01  
Trypanosoma brucei gambiense Tbg972.11.10040   elongation factor G2-like protein, putative
Trypanosoma brucei gambiense Tbg972.10.6060   Mitochondrial elongation factor G, putative
Trypanosoma brucei Tb927.10.5010   Elongation factor G1, mitochondrial, putative
Trypanosoma brucei Tb927.11.8980   Elongation factor G 2, mitochondrial, putative
Trypanosoma congolense TcIL3000.11.9290   Elongation factor G 2, mitochondrial, putative
Trypanosoma congolense TcIL3000_10_4190   Elongation factor G1, mitochondrial, putative
Trypanosoma cruzi TcCLB.509205.110   elongation factor G2-like protein, putative
Trypanosoma cruzi TcCLB.509199.10   Elongation factor G 2, mitochondrial, putative
Trypanosoma cruzi TcCLB.508601.130   Elongation factor G 2, mitochondrial, putative
Trypanosoma cruzi TcCLB.506583.60   Elongation factor G1, mitochondrial, putative
Toxoplasma gondii TGME49_223970   translation elongation and release factors (gtpases), putative
Toxoplasma gondii TGME49_260170   elongation factor EF-G, putative
Treponema pallidum TP0450   elongation factor G
Treponema pallidum TP0767   elongation factor G
Theileria parva TP03_0816   elongation factor G, putative
Theileria parva TP01_0278   translation elongation factor G 2, putative
Wolbachia endosymbiont of Brugia malayi Wbm0344   elongation factor G

Essentiality

LmjF.36.0570 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.0710 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.0710 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.0710 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.0710 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.5010 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.5010 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.5010 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.5010 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3340 Escherichia coli essential goodall
CELE_F29C12.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F29C12.4 Caenorhabditis elegans larval arrest wormbase
CELE_F29C12.4 Caenorhabditis elegans slow growth wormbase
PBANKA_0101200 Plasmodium berghei Essential plasmo
PBANKA_1447700 Plasmodium berghei Essential plasmo
TGME49_260170 Toxoplasma gondii Probably essential sidik
TGME49_223970 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.36.0570 (Leishmania major), Mitochondrial elongation factor G, putative
Title for this comment
Comment