Detailed view for LmjF.24.1390

Basic information

TDR Targets ID: 25435
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.0439 | Length (AA): 334 | MW (Da): 37208 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13499   EF-hand domain pair
PF13920   Zinc finger, C3HC4 type (RING finger)

Gene Ontology

Mouse over links to read term descriptions.
GO:0008270   zinc ion binding  
GO:0005515   protein binding  
GO:0005509   calcium ion binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 70 2ckl (B) 44 112 32.00 0.00000031 0.91 0.49 -0.18
10 59 1jm7 (A) 18 73 38.00 0.0000038 0.98 0.41 0.36
180 332 1dtl (A) 11 159 25.00 0 1 0.79 -0.92
265 332 1qx2 (A) 1 72 22.00 0.00000093 1 0.69 -2.34
8 50 2ecj (A) 10 58 53.00 0.000018 0.83 0.441243 1.01
12 57 3eb5 (A) 553 598 48.00 0.00000043 1 0.688225 -0.2
148 328 3u0k (A) 276 437 24.00 0.00035 1 0.741416 -0.29
187 250 2m97 (A) 3 66 27.00 0 0.97 0.751917 -2.15
305 334 1s6i (A) 117 146 30.00 0.035 0.1 0.25122 0.85

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_137033)

Species Accession Gene Product
Leishmania braziliensis LbrM.24.1500   hypothetical protein, conserved
Leishmania braziliensis LbrM.24.1490   hypothetical protein, conserved
Leishmania donovani LdBPK_241430.1   Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair, putative
Leishmania donovani LdBPK_241420.1   Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair, putative
Leishmania infantum LinJ.24.1420   hypothetical protein, conserved
Leishmania infantum LinJ.24.1430   hypothetical protein, conserved
Leishmania major LmjF.24.1380   hypothetical protein, conserved
Leishmania major LmjF.24.1390   hypothetical protein, conserved
Leishmania mexicana LmxM.24.1380   hypothetical protein, conserved
Leishmania mexicana LmxM.24.1390   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.8.7040   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.6850   Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair, putative
Trypanosoma cruzi TcCLB.507991.70   Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair, putative
Trypanosoma cruzi TcCLB.504035.130   Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair, putative

Essentiality

LmjF.24.1390 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.6850 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.6850 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.6850 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.6850 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Bos taurus Calmodulin 149 aa 22.8% 158 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Lmaj008579AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Lmaj008579; Recombinant protein: full-length; Source: L major; hypothetical protein, conserved ;

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.24.1390 (Leishmania major), hypothetical protein, conserved
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