Detailed view for LmjF.35.3600

Basic information

TDR Targets ID: 25541
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.3831 | Length (AA): 387 | MW (Da): 42072 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13489   Methyltransferase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008168   methyltransferase activity  
GO:0003824   catalytic activity  
GO:0005975   carbohydrate metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
66 345 1vlm (A) 3 174 18.00 0 1 0.22 0.37
79 176 2avn (A) 0 87 16.00 0.000077 0.15 0.31 -0.01
221 353 1vl5 (A) 89 201 16.00 0.0016 0.74 0.357669 0.42
254 340 4obx (A) 202 287 16.00 0.000028 0.1 0.399806 -0.23

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127178)

Species Accession Gene Product
Arabidopsis thaliana AT2G26200   S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Brugia malayi Bm1_03075   Methyltransferase-like protein
Brugia malayi Bm1_55010   Methyltransferase-like protein 4
Candida albicans CaO19.3676   similar to C terminus of S. cerevisiae actin filament binding protein
Candida albicans CaO19.11160   similar to C terminus of S. cerevisiae actin filament binding protein
Caenorhabditis elegans CELE_ZK1058.5   Protein ZK1058.5
Caenorhabditis elegans CELE_Y53F4B.42   Protein Y53F4B.42
Cryptosporidium hominis Chro.80491   FLJ12760 protein
Cryptosporidium parvum cgd8_4270   conserved protein, methylase
Dictyostelium discoideum DDB_G0275041   hypothetical protein
Dictyostelium discoideum DDB_G0274197   methyltransferase type 12 domain-containing protein
Drosophila melanogaster Dmel_CG13929   methyltransferase-like
Drosophila melanogaster Dmel_CG34195   CG34195 gene product from transcript CG34195-RA
Echinococcus granulosus EgrG_000506200   Methyltransferase protein 2
Entamoeba histolytica EHI_069570   methyltransferase-like protein 2, putative
Echinococcus multilocularis EmuJ_000506200   Methyltransferase protein 2
Giardia lamblia GL50803_9528   Methyltransferase like 2
Homo sapiens ENSG00000206562   methyltransferase like 6
Homo sapiens ENSG00000087995   methyltransferase like 2A
Homo sapiens ENSG00000123600   methyltransferase like 8
Homo sapiens ENSG00000165055   methyltransferase like 2B
Leishmania braziliensis LbrM.34.3560   hypothetical protein, conserved
Leishmania donovani LdBPK_353650.1   methyltransferase domain containing protein, putative
Leishmania infantum LinJ.35.3650   hypothetical protein, conserved
Leishmania major LmjF.35.3600   hypothetical protein, conserved
Leishmania mexicana LmxM.34.3600   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_11363   hypothetical protein
Loa Loa (eye worm) LOAG_02021   hypothetical protein
Loa Loa (eye worm) LOAG_04036   hypothetical protein
Mus musculus 52686   methyltransferase like 2
Mus musculus ENSMUSG00000041975   methyltransferase like 8
Mus musculus 67011   methyltransferase like 6
Neospora caninum NCLIV_022570   hypothetical protein
Oryza sativa 4331587   Os03g0143000
Onchocerca volvulus OVOC10445   Putative methyltransferase NSUN5
Plasmodium berghei PBANKA_1461000   methyltransferase, putative
Plasmodium falciparum PF3D7_1248100   methyltransferase, putative
Plasmodium knowlesi PKNH_1467800   methyltransferase, putative
Plasmodium vivax PVX_101295   methyltransferase, putative
Plasmodium yoelii PY01571   Drosophila melanogaster CG13929 gene product-related
Saccharomyces cerevisiae YOR239W   Abp140p
Schistosoma japonicum Sjp_0216710   ko:K00599 GA12634-PA [EC:2.1.1.-], putative
Schistosoma mansoni Smp_037930   methyltransferase-related
Schistosoma mansoni Smp_061500   methyltransferase-related
Schmidtea mediterranea mk4.000243.04   Methyltransferase-like protein
Trypanosoma brucei gambiense Tbg972.9.7000   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.11750   methyltransferase domain containing protein, putative
Trypanosoma cruzi TcCLB.510667.50   methyltransferase domain containing protein, putative
Trypanosoma cruzi TcCLB.507009.110   methyltransferase domain containing protein, putative
Toxoplasma gondii TGME49_202360   methylase, putative

Essentiality

LmjF.35.3600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.2860 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.2860 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.2860 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.2860 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1461000 Plasmodium berghei Dispensable plasmo
TGME49_202360 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier LmjF.35.3600 (Leishmania major), hypothetical protein, conserved
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