Detailed view for ML2237

Basic information

TDR Targets ID: 255743
Mycobacterium leprae, conserved hypothetical protein (HIT family)

Source Database / ID:  Leproma 

pI: 5.5049 | Length (AA): 134 | MW (Da): 14712 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01230   HIT domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 134 3p0t (A) 1 134 82.00 0 1 2.0858 -1.77
26 98 1fit (A) 24 101 27.00 0.0021 0.27 0.800576 -0.3

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126794)

Species Accession Gene Product
Arabidopsis thaliana AT5G48545   protein histidine triad nucleotide-binding 3
Arabidopsis thaliana AT1G31160   histidine triad nucleotide-binding 2 protein
Arabidopsis thaliana AT3G56490   HIS triad family protein 3
Babesia bovis BBOV_III011870   HIT domain containing protein
Brugia malayi Bm1_56730   Hypothetical HIT-like protein F21C3.3
Candida albicans CaO19.2341   potential histidine triad nucleotide-binding protein similar to S. cerevisiae HNT1 (YDL125C) Kin28p-interacting adenosine 5'-mon
Candida albicans CaO19.9877   similar to the histidine triad superfamily of nucleotide-binding proteins
Caenorhabditis elegans CELE_F21C3.3   Protein HINT-1
Cryptosporidium hominis Chro.10184   hypothetical protein
Cryptosporidium parvum cgd1_1600   histidine triad (HIT) family zinc binding protein
Chlamydia trachomatis CT_385   Hit family hydrolase
Dictyostelium discoideum DDB_G0289439   histidine triad family protein
Dictyostelium discoideum DDB_G0289391   histidine triad family protein
Drosophila melanogaster Dmel_CG2862   CG2862 gene product from transcript CG2862-RA
Escherichia coli b1103   purine nucleoside phosphoramidase, dadA activator protein
Echinococcus granulosus EgrG_000597600   serine:threonine protein kinase KDX1
Entamoeba histolytica EHI_093910   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000597600   serine:threonine protein kinase KDX1
Giardia lamblia GL50803_4156   HIT family protein
Homo sapiens 3094   histidine triad nucleotide binding protein 1
Homo sapiens ENSG00000137133   histidine triad nucleotide binding protein 2
Leishmania braziliensis LbrM.14.0240   hypothetical protein, conserved
Leishmania donovani LdBPK_140240.1   HIT domain containing protein, putative
Leishmania infantum LinJ.14.0240   hypothetical protein, conserved
Leishmania major LmjF.14.0240   hypothetical protein, conserved
Leishmania mexicana LmxM.14.0240   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_07417   hypothetical protein
Mycobacterium leprae ML2237   conserved hypothetical protein (HIT family)
Mus musculus ENSMUSG00000028470   histidine triad nucleotide binding protein 2
Mus musculus ENSMUSG00000020267   histidine triad nucleotide binding protein 1
Mycobacterium tuberculosis Rv1262c   Hypothetical hit-like protein
Mycobacterium tuberculosis Rv0759c   Conserved hypothetical protein
Mycobacterium ulcerans MUL_0465   hypothetical protein
Mycobacterium ulcerans MUL_4479   Hit-like protein
Neospora caninum NCLIV_018300   HIT family protein, related
Oryza sativa 4351835   Os12g0233300
Oryza sativa 9270866   Os11g0295000
Oryza sativa 4332685   Os03g0322500
Plasmodium berghei PBANKA_0713300   protein kinase c inhibitor-like protein, putative
Plasmodium falciparum PF3D7_0817500   protein kinase c inhibitor-like protein, putative
Plasmodium knowlesi PKNH_0502900   protein kinase c inhibitor-like protein, putative
Plasmodium vivax PVX_089540   protein kinase c inhibitor-like protein, putative
Plasmodium yoelii PY05168   putative protein kinase C interacting protein 1
Saccharomyces cerevisiae YDL125C   Hnt1p
Schistosoma japonicum Sjp_0218600   ko:K06069 atypical protein kinase C, putative
Schistosoma mansoni Smp_048650   histidine triad (hit) protein
Schmidtea mediterranea mk4.003135.01   Histidine triad nucleotide-binding protein 2, mitochondrial
Schmidtea mediterranea mk4.003480.02   Histidine triad nucleotide-binding protein 2, mitochondrial
Schmidtea mediterranea mk4.014420.00   Histidine triad nucleotide-binding protein 2, mitochondrial
Trypanosoma brucei gambiense Tbg972.7.5070   adenosine 5-monophosphoramidase, putative
Trypanosoma brucei Tb927.7.4480   adenosine 5'-monophosphoramidase, putative
Trypanosoma cruzi TcCLB.506605.59   adenosine 5'-monophosphoramidase, putative
Trypanosoma cruzi TcCLB.511239.49   adenosine 5'-monophosphoramidase, putative
Toxoplasma gondii TGME49_243580   Hit family protein involved in cell-cycle regulation, putative
Theileria parva TP01_0012   protein kinase C interacting protein 1, putative
Wolbachia endosymbiont of Brugia malayi Wbm0240   HIT family hydrolase

Essentiality

ML2237 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1283 Mycobacterium tuberculosis non-essential nmpdr
Tb927.7.4480 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.4480 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.4480 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.4480 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b1103 Escherichia coli non-essential goodall
PBANKA_0713300 Plasmodium berghei Dispensable plasmo
TGME49_243580 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier ML2237 (Mycobacterium leprae), conserved hypothetical protein (HIT family)
Title for this comment
Comment