Detailed view for ML2118c
Basic information
Mycobacterium leprae, POSSIBLE ENOYL-CoA HYDRATASE ECHA6 (ENOYL HYDRASE) (UNSATURATED ACYL-CoA HYDRATASE) (CROTONASE)
Source Database / ID: Leproma
pI: 6.1198 |
Length (AA): 247 |
MW (Da): 26654 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Network
Pfam domains
Gene Ontology
GO:0008152 metabolic process
Metabolic Pathways
Valine, leucine and isoleucine degradation (KEGG)
Geraniol degradation (KEGG)
Lysine degradation (KEGG)
Phenylalanine metabolism (KEGG)
Benzoate degradation via hydroxylation (KEGG)
Tryptophan metabolism (KEGG)
beta-Alanine metabolism (KEGG)
1,4-Dichlorobenzene degradation (KEGG)
Benzoate degradation via CoA ligation (KEGG)
Propanoate metabolism (KEGG)
Butanoate metabolism (KEGG)
Limonene and pinene degradation (KEGG)
Caprolactam degradation (KEGG)
Global map (KEGG)
Biosynthesis of secondary metabolites (KEGG)
Microbial metabolism in diverse environments (KEGG)
Biosynthesis of antibiotics (KEGG)
Fatty acid metabolism (KEGG)
Structural information
Modbase 3D models:
There is 1 model calculated for this protein. More info on
this model, including the
model itself is available at:
Modbase
| Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 247 | 5dtp (B) | 1 | 243 | 87.00 | 0 | 1 | 2.0155 | -1.15 |
Help me make sense of these data.
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
- 3HE2:
-
Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
Expression
Orthologs
Ortholog group members (OG5_221959)
| Species | Accession | Gene Product |
|---|---|---|
| Mycobacterium leprae | ML2118c | POSSIBLE ENOYL-CoA HYDRATASE ECHA6 (ENOYL HYDRASE) (UNSATURATED ACYL-CoA HYDRATASE) (CROTONASE) |
| Mycobacterium tuberculosis | Rv0905 | Possible enoyl-CoA hydratase EchA6 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) |
| Mycobacterium ulcerans | MUL_0236 | enoyl-CoA hydratase |
Essentiality
| Gene/Ortholog | Organism | Phenotype | Source Study |
|---|---|---|---|
| mtu921 | Mycobacterium tuberculosis | non-essential | nmpdr |
-
keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection -
nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource -
gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84 -
wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170 -
yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database -
shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan -
alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924 -
neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs -
blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
Phenotypes and Validation (curated)
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Annotated validation
Associated compounds / Druggability
Known modulators for this target
Predicted associations
By orthology with druggable targets
By sequence similarity to non orthologous druggable targets
Obtained from network model
Assayability
Assay information
- Assay for Crotonase (4.2.1.17 ) Sigma-Aldrich
- Automatic link to known assays based on EC numbers.
- BRENDA Assay
- An enzyme with this EC number or name or sequence has been assayed in Mycobacterium smegmatis ( 1 )
Reagent availability
- Reagent:
-
Target Type Source Notes ML2118c purified protein BRENDA A protein with this EC number or name or sequence has been purified from Mycobacterium smegmatis ( 1 )
Bibliographic References