Detailed view for ML0414c

Basic information

TDR Targets ID: 256272
Mycobacterium leprae, Possible trehalose-6-phosphate phosphatase OtsB2 (Trehalose-phosphatase) (TPP)
Source Database / ID:  Leproma 

pI: 5.3716 | Length (AA): 429 | MW (Da): 45888 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02358   Trehalose-phosphatase

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0005992   trehalose biosynthetic process  

Metabolic Pathways

Starch and sucrose metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 427 5gvx (A) 6 386 68.00 0 1 1.66641 -0.31
171 292 2o2x (A) 26 167 19.00 0 0.94 0.420682 -0.77

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_130248)

Species Accession Gene Product
Arabidopsis thaliana AT5G65140   probable trehalose-phosphate phosphatase J
Arabidopsis thaliana AT1G78090   trehalose-6-phosphate phosphatase
Arabidopsis thaliana AT1G35910   probable trehalose-phosphate phosphatase D
Arabidopsis thaliana AT4G12430   probable trehalose-phosphate phosphatase F
Arabidopsis thaliana AT4G39770   probable trehalose-phosphate phosphatase H
Arabidopsis thaliana AT1G22210   probable trehalose-phosphate phosphatase C
Arabidopsis thaliana AT5G10100   probable trehalose-phosphate phosphatase I
Arabidopsis thaliana AT2G22190   probable trehalose-phosphate phosphatase E
Arabidopsis thaliana AT4G22590   trehalose-phosphate phosphatase G
Arabidopsis thaliana AT5G51460   trehalose-phosphate phosphatase A
Drosophila melanogaster Dmel_CG5171   CG5171 gene product from transcript CG5171-RD
Escherichia coli b1897   trehalose-6-phosphate phosphatase, biosynthetic
Mycobacterium leprae ML0414c   Possible trehalose-6-phosphate phosphatase OtsB2 (Trehalose-phosphatase) (TPP)
Mycobacterium leprae ML0393c   Probable hydrolase
Mycobacterium tuberculosis Rv2006   Probable trehalose-6-phosphate phosphatase OtsB1 (trehalose-phosphatase) (TPP)
Mycobacterium tuberculosis Rv3372   Trehalose 6-phosphate phosphatase OtsB2 (trehalose-phosphatase) (TPP)
Mycobacterium ulcerans MUL_0921   trehalose-6-phosphate phosphatase OtsB2
Oryza sativa 4340524   Os06g0222100
Oryza sativa 4349333   Os10g0553300
Oryza sativa 4346885   Os09g0369400
Oryza sativa 4330753   Os02g0753000
Oryza sativa 4332996   Os03g0386500
Oryza sativa 9267122   Os08g0409100
Oryza sativa 4343959   Os07g0624600
Oryza sativa 4330221   Os02g0661100

Essentiality

ML0414c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b1897 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Mycobacterium smegmatis ( 2 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    ML0414c cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Mycobacterium tuberculosis ( 1 )

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier ML0414c (Mycobacterium leprae), Possible trehalose-6-phosphate phosphatase OtsB2 (Trehalose-phosphatase) (TPP)
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