Detailed view for ML1678c
Basic information
Mycobacterium leprae, PROBABLE D-ALANINE--D-ALANINE LIGASE DDLA (D-ALANYLALANINE SYNTHETASE) (D-ALA-D-ALA LIGASE)
Source Database / ID: Leproma
pI: 4.8064 |
Length (AA): 384 |
MW (Da): 40949 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Network
Gene Ontology
GO:0046872 metal ion binding
GO:0008716 D-alanine-D-alanine ligase activity
GO:0005524 ATP binding
Metabolic Pathways
Peptidoglycan biosynthesis (KEGG)
Global map (KEGG)
Vancomycin resistance (KEGG)
Structural information
Modbase 3D models:
There is 1 model calculated for this protein. More info on
this model, including the
model itself is available at:
Modbase
| Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
|---|---|---|---|---|---|---|---|---|---|
| 17 | 383 | 3lwb (B) | 10 | 372 | 84.00 | 0 | 1 | 1.66183 | -0.06 |
Help me make sense of these data.
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
- 3LWB:
-
Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
Expression
Orthologs
Ortholog group members (OG5_130117)
| Species | Accession | Gene Product |
|---|---|---|
| Arabidopsis thaliana | AT3G08840 | D-alanine--D-alanine ligase family protein |
| Escherichia coli | b0381 | D-alanine-D-alanine ligase A |
| Escherichia coli | b0092 | D-alanine:D-alanine ligase |
| Mycobacterium leprae | ML1678c | PROBABLE D-ALANINE--D-ALANINE LIGASE DDLA (D-ALANYLALANINE SYNTHETASE) (D-ALA-D-ALA LIGASE) |
| Mycobacterium tuberculosis | Rv2981c | Probable D-alanine--D-alanine ligase DdlA (D-alanylalanine synthetase) (D-ala-D-ala ligase) |
| Mycobacterium ulcerans | MUL_1976 | D-alanyl-alanine synthetase A |
| Oryza sativa | 4344370 | Os07g0691200 |
| Treponema pallidum | TP0670 | D-alanyl-alanine synthetase A |
| Wolbachia endosymbiont of Brugia malayi | Wbm0570 | D-alanine--D-alanine ligase |
Essentiality
| Gene/Ortholog | Organism | Phenotype | Source Study |
|---|---|---|---|
| b0092 | Escherichia coli | non-essential | goodall |
| b0381 | Escherichia coli | non-essential | goodall |
-
shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan -
wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170 -
yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database -
blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930 -
gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84 -
alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924 -
nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource -
keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection -
neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
Phenotypes and Validation (curated)
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Annotated validation
Associated compounds / Druggability
Known modulators for this target
Predicted associations
By orthology with druggable targets
| Species | Known druggable target | Linked compounds | Reference |
|---|---|---|---|
| Helicobacter pylori (strain HPAG1) | D-alanine--D-alanine ligase | Compounds | References |
| Escherichia coli | D-alanine:D-alanine ligase | Compounds | References |
By sequence similarity to non orthologous druggable targets
Obtained from network model
Assayability
Assay information
Reagent availability
- Reagent:
-
Target Type Source Notes ML1678c cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Mycobacterium tuberculosis ( 1 )
Bibliographic References