Detailed view for TGME49_321340

Basic information

TDR Targets ID: 257329
Toxoplasma gondii, membrane protein, putative
Source Database / ID:  ToxoDB 

pI: 8.5363 | Length (AA): 3811 | MW (Da): 417033 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 3

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05346   Eukaryotic membrane protein family

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1732 1969 5cwm (A) 10 226 17.00 0 0.08 0.173351 -0.64
1817 1934 5hmo (A) 814 922 20.00 0.17 0.01 0.226563 -0.86
3191 3273 4o9b (A) 249 319 20.00 0 0.03 0.246679 -2.26
3227 3323 5hmo (A) 806 903 30.00 0.6 0 0.470753 -1.88

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 Oocyst. Gregory Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128307)

Species Accession Gene Product
Arabidopsis thaliana AT1G67960   protein POLLEN DEFECTIVE IN GUIDANCE 1
Babesia bovis BBOV_II004690   eukaryotic membrane protein, putative
Brugia malayi Bm1_50325   FLJ90013 protein
Candida albicans CaO19.3658   similar to S. cerevisiae YER140W
Candida albicans CaO19.11142   similar to S. cerevisiae YER140W
Caenorhabditis elegans CELE_F26F2.7   Protein F26F2.7
Cryptosporidium hominis Chro.40200   hypothetical protein
Cryptosporidium parvum cgd4_1760   hypothetical protein with 2 transmembrane domains
Dictyostelium discoideum DDB_G0277313   DUF747 family protein
Drosophila melanogaster Dmel_CG7218   CG7218 gene product from transcript CG7218-RB
Echinococcus granulosus EgrG_000890200   enhancer of rudimentary
Echinococcus granulosus EgrG_000890100   Membrane proteinTapt1 CMV receptor
Entamoeba histolytica EHI_100500   hypothetical membrane-spanning protein
Echinococcus multilocularis EmuJ_000890100   Membrane protein,Tapt1 CMV receptor
Echinococcus multilocularis EmuJ_000890200   enhancer of rudimentary
Echinococcus multilocularis EmuJ_000011400   Membrane protein,Tapt1 CMV receptor
Homo sapiens ENSG00000169762   transmembrane anterior posterior transformation 1
Leishmania braziliensis LbrM.07.0150   hypothetical protein, conserved
Leishmania donovani LdBPK_070310.1   Eukaryotic membrane protein family, putative
Leishmania infantum LinJ.07.0310   hypothetical protein, conserved
Leishmania major LmjF.07.0150   hypothetical protein, conserved
Leishmania mexicana LmxM.07.0150   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_01351   hypothetical protein
Mus musculus ensembl-mmu:ENSMUSG00000046985   transmembrane anterior posterior transformation 1
Neospora caninum NCLIV_004580   hypothetical protein
Oryza sativa 4333908   Os03g0715400
Onchocerca volvulus OVOC12856  
Onchocerca volvulus OVOC7062  
Plasmodium berghei PBANKA_1216600   cyclic amine resistance locus protein, putative
Plasmodium falciparum PF3D7_0321900   cyclic amine resistance locus protein
Plasmodium knowlesi PKNH_0818900   cyclic amine resistance locus protein, putative
Plasmodium vivax PVX_095140   cyclic amine resistance locus protein, putative
Plasmodium yoelii PY03667   hypothetical protein
Saccharomyces cerevisiae YER140W   Emp65p
Schistosoma japonicum Sjp_0308130   Enhancer of rudimentary homolog, putative
Schistosoma mansoni Smp_175200   hypothetical protein
Schmidtea mediterranea mk4.001853.06   Protein TAPT1 homolog
Schmidtea mediterranea mk4.000177.12   Enhancer of rudimentary homolog
Trypanosoma brucei gambiense Tbg972.8.1430   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.1800   Eukaryotic membrane protein family, putative
Trypanosoma congolense TcIL3000_8_1730   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.503565.20   Eukaryotic membrane protein family, putative
Trypanosoma cruzi TcCLB.511393.40   Eukaryotic membrane protein family, putative
Toxoplasma gondii TGME49_321340   membrane protein, putative
Theileria parva TP02_0285   hypothetical protein, conserved

Essentiality

TGME49_321340 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.1800 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.1800 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.1800 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.1800 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F26F2.7 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_1216600 Plasmodium berghei Essential plasmo
TGME49_321340 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_321340 (Toxoplasma gondii), membrane protein, putative
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