Detailed view for LmjF.34.3850

Basic information

TDR Targets ID: 25739
Leishmania major, aquaporin 9, putative,major intrisic-like protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1209 | Length (AA): 230 | MW (Da): 24814 | Paralog Number: 2

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 6

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00230   Major intrinsic protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0015267   channel activity  
GO:0016020   membrane  
GO:0005215   transporter activity  
GO:0055085   transmembrane transport  
GO:0006810   transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 216 1z98 (A) 34 265 24.00 0 0.34 1.33 -2.39
3 223 1tm8 (A) 7 231 25.00 0 0.68 1.39 -1.88
8 214 1ldf (A) 10 254 33.00 0 0.82 1.21 -1.72
2 216 2f2b (A) 1 245 29.00 0 0.85 1.16868 -0.51
5 226 3d9s (A) 10 242 27.00 0 0.84 1.21232 -0.02
122 169 4l6v (L) 90 136 47.00 0.75 0.21 0.572096 1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126615)

Species Accession Gene Product
Arabidopsis thaliana AT4G19030   aquaporin NIP1-1
Arabidopsis thaliana AT4G01470   3
Arabidopsis thaliana AT1G73190   1
Arabidopsis thaliana AT4G18910   2
Arabidopsis thaliana AT1G17810   beta-tonoplast intrinsic protein
Arabidopsis thaliana AT3G16240   aquaporin TIP2-1
Arabidopsis thaliana AT2G34390   1
Arabidopsis thaliana AT3G06100   1
Arabidopsis thaliana AT2G25810   1
Arabidopsis thaliana AT5G37820   2
Arabidopsis thaliana AT1G31885   1
Arabidopsis thaliana AT5G37810   1
Arabidopsis thaliana AT2G16850   8
Brugia malayi Bm1_03100   Major intrinsic protein
Brugia malayi Bm1_03135   Major intrinsic protein
Brugia malayi Bm1_03110   Major intrinsic protein
Brugia malayi Bm1_03140   Major intrinsic protein
Brugia malayi Bm1_57250   Major intrinsic protein
Caenorhabditis elegans CELE_M02F4.8   Protein AQP-7
Caenorhabditis elegans CELE_C01G6.1   Protein AQP-2, isoform B
Caenorhabditis elegans CELE_K02G10.7   Protein AQP-8, isoform A
Caenorhabditis elegans CELE_Y69E1A.7   Protein AQP-3
Dictyostelium discoideum DDB_G0267378   major intrinsic protein family protein
Dictyostelium discoideum DDB_G0280537   aquaporin-like protein
Drosophila melanogaster Dmel_CG17664   CG17664 gene product from transcript CG17664-RC
Drosophila melanogaster Dmel_CG9023   CG9023 gene product from transcript CG9023-RG
Drosophila melanogaster Dmel_CG4019   CG4019 gene product from transcript CG4019-RA
Drosophila melanogaster Dmel_CG17662   CG17662 gene product from transcript CG17662-RB
Escherichia coli b0875   aquaporin Z
Escherichia coli b3927   glycerol facilitator
Echinococcus granulosus EgrG_001190800   Aquaporin 9 AQP 9 Small solute channel 1
Echinococcus granulosus EgrG_000153200   Aquaporin 9 AQP 9 Small solute channel 1
Echinococcus multilocularis EmuJ_000153200   Glycosyltransferase, ALG10
Echinococcus multilocularis EmuJ_001190800   Aquaporin 9 (AQP 9) (Small solute channel 1)
Homo sapiens ENSG00000103569   aquaporin 9
Homo sapiens ENSG00000165272   aquaporin 3 (Gill blood group)
Homo sapiens ENSG00000143595   aquaporin 10
Homo sapiens ENSG00000171885   aquaporin 4
Homo sapiens 362   aquaporin 5
Homo sapiens 101930168   putative aquaporin-7-like protein 3-like
Homo sapiens ENSG00000165269   aquaporin 7
Homo sapiens ENSG00000259916   putative aquaporin-7-like protein 3-like
Leishmania braziliensis LbrM.31.0020   aquaglyceroporin
Leishmania braziliensis LbrM.32.2610   aquaporin-like protein
Leishmania braziliensis LbrM.20.3460   aquaporin 9, putative,major intrisic-like protein
Leishmania donovani LdBPK_310030.1   Aquaglyceroporin 1
Leishmania donovani LdBPK_343660.1   aquaporin 9, putative
Leishmania donovani LdBPK_322500.1   aquaporin-like protein
Leishmania infantum LinJ.34.3660   aquaporin 9, putative,major intrisic-like protein
Leishmania infantum LinJ.32.2500   aquaporin-like protein
Leishmania infantum LinJ.31.0030   aquaglyceroporin
Leishmania major LmjF.31.0020   aquaglyceroporin
Leishmania major LmjF.34.3850   aquaporin 9, putative,major intrisic-like protein
Leishmania major LmjF.32.2380   aquaporin-like protein
Leishmania mexicana LmxM.31.2380   aquaporin-like protein
Leishmania mexicana LmxM.33.3850   aquaporin 9, putative,major intrisic-like protein
Leishmania mexicana LmxM.30.0020   aquaglyceroporin
Loa Loa (eye worm) LOAG_01613   major intrinsic protein
Loa Loa (eye worm) LOAG_01620   major intrinsic protein
Loa Loa (eye worm) LOAG_11786   major intrinsic protein
Mus musculus ENSMUSG00000024411   aquaporin 4
Mus musculus ENSMUSG00000032204   aquaporin 9
Mus musculus ENSMUSG00000028435   aquaporin 3
Mus musculus ENSMUSG00000028427   aquaporin 7
Mus musculus ENSMUSG00000044217   aquaporin 5
Oryza sativa 4324277   Os01g0232100
Oryza sativa 4324303   Os01g0975900
Oryza sativa 4340941   Os06g0336200
Oryza sativa 4328814   Os02g0232900
Oryza sativa 4338163   Os05g0231700
Oryza sativa 4348981   Os10g0492600
Oryza sativa 4324276   Os01g0232000
Oryza sativa 4327074   Os01g0202800
Onchocerca volvulus OVOC11208  
Onchocerca volvulus OVOC11198  
Onchocerca volvulus OVOC11207  
Plasmodium berghei PBANKA_0915600   aquaglyceroporin
Plasmodium falciparum PF3D7_1132800   aquaglyceroporin
Plasmodium knowlesi PKNH_0931000   aquaglyceroporin, putative
Plasmodium vivax PVX_092245   aquaglyceroporin, putative
Plasmodium yoelii PY05950   aquaglyceroporin
Saccharomyces cerevisiae YLL043W   Fps1p
Saccharomyces cerevisiae YFL054C   hypothetical protein
Schistosoma japonicum Sjp_0218290   ko:K09876 aquaporin-3, putative
Schistosoma japonicum Sjp_0055350   Aquaporin-9, putative
Schistosoma japonicum Sjp_0112230   IPR000425,Major intrinsic protein,domain-containing
Schistosoma japonicum Sjp_0209930   ko:K09877 aquaporin-9, putative
Schistosoma japonicum Sjp_0055360   Aquaporin-9, putative
Schistosoma mansoni Smp_005720   aquaporin-3
Schistosoma mansoni Smp_005740   aquaporin-3
Schmidtea mediterranea mk4.003793.01  
Schmidtea mediterranea mk4.014833.01  
Schmidtea mediterranea mk4.002202.00  
Trypanosoma brucei gambiense Tbg972.10.16540   aquaglyceroporin (small solute channel), putative
Trypanosoma brucei gambiense Tbg972.6.1170   aquaporin 3, putative
Trypanosoma brucei Tb927.10.14160   Aquaglyceroporin 3
Trypanosoma brucei Tb927.6.1520   Aquaglyceroporin 1
Trypanosoma congolense TcIL3000_10_12040   aquaporin 3, putative
Trypanosoma cruzi TcCLB.504159.50   aquaporin-like protein, putative
Trypanosoma cruzi TcCLB.508257.140   Aquaporin 2
Trypanosoma cruzi TcCLB.506821.170   aquaporin-like protein, putative

Essentiality

LmjF.34.3850 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.14160 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.14160 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.14160 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.14160 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.6.1520 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.1520 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.1520 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.1520 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0875 Escherichia coli non-essential goodall
b3927 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.34.3850 (Leishmania major), aquaporin 9, putative,major intrisic-like protein
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