Detailed view for TGME49_316110

Basic information

TDR Targets ID: 257419
Toxoplasma gondii, ribosomal protein RPS15
Source Database / ID:  ToxoDB 

pI: 9.9823 | Length (AA): 679 | MW (Da): 73246 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00312   Ribosomal protein S15

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
17 85 2kr0 (A) 192 265 41.00 0.017 0.06 0.34012 0.87
350 445 1w9r (A) 11 93 34.00 0.16 0.12 0.575884 -2.53
367 441 3u59 (A) 14 84 27.00 0.73 0.08 0.660957 -3.43
507 580 2vqe (O) 15 88 42.00 0.000000075 0.97 0.699484 -0.94
507 580 4a5u (B) 14 87 44.00 0 0.98 0.523984 -0.63

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_127663)

Species Accession Gene Product
Arabidopsis thaliana AT1G80620   S15/NS1, RNA-binding protein
Arabidopsis thaliana AT1G15810   S15/NS1, RNA-binding protein
Babesia bovis BBOV_III007430   30S ribosomal protein S15
Candida albicans CaO19.2520   likely mitochondrial ribosomal protein similar to S. cerevisiae MRPS28 (YDR337W) small subunit protein (E. coli S15)
Candida albicans CaO19.10056   likely mitochondrial ribosomal protein similar to S. cerevisiae MRPS28 (YDR337W) small subunit protein (E. coli S15)
Chlamydia trachomatis CT_843   30S ribosomal protein S15
Dictyostelium discoideum DDB_G0289469   hypothetical protein
Drosophila melanogaster Dmel_CG4207   CG4207 gene product from transcript CG4207-RA
Escherichia coli b3165   30S ribosomal subunit protein S15
Echinococcus granulosus EgrG_000191300   expressed conserved protein
Echinococcus multilocularis EmuJ_000191300   expressed conserved protein
Homo sapiens ENSG00000116898   mitochondrial ribosomal protein S15
Mycobacterium leprae ML0853   PROBABLE 30S RIBOSOMAL PROTEIN S15 RPSO
Mus musculus ENSMUSG00000028861   mitochondrial ribosomal protein S15
Mycobacterium tuberculosis Rv2785c   30S ribosomal protein S15 RpsO
Mycobacterium ulcerans MUL_2147   30S ribosomal protein S15
Neospora caninum NCLIV_058640   ribosomal protein S15, putative
Oryza sativa 4331046   Os02g0803200
Plasmodium berghei PBANKA_0940800   apicoplast ribosomal protein S15 precursor, putative
Plasmodium falciparum PF3D7_1106100   apicoplast ribosomal protein S15 precursor, putative
Plasmodium knowlesi PKNH_0903700   ribosomal protein S15, putative
Plasmodium vivax PVX_090980   30S ribosomal protein S15, putative
Plasmodium yoelii PY03370   ribosomal protein S15, putative
Saccharomyces cerevisiae YDR337W   mitochondrial 37S ribosomal protein MRPS28
Schistosoma japonicum Sjp_0061660   IPR009068,S15/NS1, RNA-binding,domain-containing
Schistosoma mansoni Smp_074210   hypothetical protein
Schmidtea mediterranea mk4.034460.00  
Toxoplasma gondii TGME49_316110   ribosomal protein RPS15
Treponema pallidum TP0887   30S ribosomal protein S15
Theileria parva TP04_0924   hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0020   30S ribosomal protein S15

Essentiality

TGME49_316110 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
b3165 Escherichia coli essential goodall
PBANKA_0940800 Plasmodium berghei Essential plasmo
TGME49_316110 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TGME49_316110 (Toxoplasma gondii), ribosomal protein RPS15
Title for this comment
Comment