Detailed view for TGME49_211480

Basic information

TDR Targets ID: 257429
Toxoplasma gondii, GTP-binding protein engA, putative
Source Database / ID:  ToxoDB 

pI: 9.438 | Length (AA): 1175 | MW (Da): 130745 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01926   50S ribosome-binding GTPase
PF14714   KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 12 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
533 811 1xzp (A) 96 363 31.00 0.000000000016 0.99 0.5 0.67
650 1171 1mky (A) 2 439 24.00 0 1 0.1 0.18
651 813 2cxx (A) 3 176 24.00 0 1 0.32 -0.32
261 316 5ep0 (A) 286 356 41.00 0.31 0.46 0.16716 0.67
456 678 4x0j (A) 40 261 10.00 0 0.02 0.359287 -1.03
501 652 5cwh (A) 2 152 9.00 0 0.01 0.338762 -1.56
677 1161 5dn8 (A) 1 433 32.00 0 1 0.536766 0.65
678 1154 5dn8 (A) 2 426 39.00 0 1 0.635457 0.51
679 1159 4dcu (A) 4 433 41.00 0 1 0.651362 0.27
897 1119 4awx (A) 2 197 28.00 0.069 1 0.199287 1.11
899 991 2gj8 (A) 217 304 42.00 0.00000000054 0.73 0.540649 -0.28
900 1075 3iev (A) 5 176 30.00 0 1 0.536787 -0.65

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128684)

Species Accession Gene Product
Arabidopsis thaliana AT5G39960   GTP-binding protein
Arabidopsis thaliana AT3G12080   putative double era-like GTPase
Babesia bovis BBOV_I004580   GTP-binding protein engA, putative
Chlamydia trachomatis CT_703   GTPase Der
Dictyostelium discoideum DDB_G0279259   hypothetical protein
Escherichia coli b2511   GTPase
Leishmania braziliensis LbrM.26.0300   ras-like small GTPases, putative
Leishmania donovani LdBPK_260260.1   ras-like small GTPases, putative
Leishmania infantum LinJ.26.0260   ras-like small GTPases, putative
Leishmania major LmjF.26.0270   ras-like small GTPases, putative
Leishmania mexicana LmxM.26.0270   GTP-binding protein-like protein
Mycobacterium leprae ML1372   PROBABLE GTP-BINDING PROTEIN ENGA
Mycobacterium tuberculosis Rv1713   Probable GTP-binding protein EngA
Mycobacterium ulcerans MUL_3232   GTP-binding protein EngA
Neospora caninum NCLIV_021910   small GTP-binding protein, putative
Neospora caninum NCLIV_011480   hypothetical protein
Oryza sativa 9266550   Os01g0225200
Oryza sativa 4350988   Os11g0638000
Plasmodium berghei PBANKA_1432900   GTP-binding protein, putative
Plasmodium falciparum PF3D7_1217300   GTP-binding protein, putative
Plasmodium knowlesi PKNH_1436900   GTP-binding protein, putative
Plasmodium vivax PVX_123540   GTP-binding protein, putative
Plasmodium yoelii PY05368   GTP binding protein-related
Trypanosoma brucei gambiense Tbg972.7.1650   GTP-binding protein, putative
Trypanosoma brucei Tb927.7.1640   ras-like small GTPase, putative
Trypanosoma congolense TcIL3000_7_1190   GTP-binding protein, putative
Trypanosoma cruzi TcCLB.509509.60   small GTP-binding protein, putative
Trypanosoma cruzi TcCLB.508207.200   small GTP-binding protein, putative
Toxoplasma gondii TGME49_203020   ribosome biogenesis GTPase Der protein
Toxoplasma gondii TGME49_211480   GTP-binding protein engA, putative
Treponema pallidum TP0689   GTP-binding protein EngA
Theileria parva TP03_0502   GTP-binding protein, putative
Wolbachia endosymbiont of Brugia malayi Wbm0586   GTP-binding protein EngA

Essentiality

TGME49_211480 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.1640 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.1640 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.1640 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.1640 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2511 Escherichia coli essential goodall
PBANKA_1432900 Plasmodium berghei Essential plasmo
TGME49_211480 this record Toxoplasma gondii Probably essential sidik
TGME49_203020 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TGME49_211480 (Toxoplasma gondii), GTP-binding protein engA, putative
Title for this comment
Comment