Detailed view for TGME49_249390

Basic information

TDR Targets ID: 257474
Toxoplasma gondii, glutamate/leucine/phenylalanine/valine dehydrogenase family protein
Source Database / ID:  ToxoDB 

pI: 7.4575 | Length (AA): 1206 | MW (Da): 135701 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00208   Glutamate/Leucine/Phenylalanine/Valine dehydrogenase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  
GO:0006520   amino acid metabolic process  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
113 171 2vqa (A) 195 253 34.00 0.67 0.03 0.273622 1.08
472 589 2vih (A) 28 138 28.00 0.78 0.27 0.179544 0.75
606 1094 2yfh (A) 4 446 16.00 0 1 0.455973 0.63
658 1138 3mw9 (A) 52 489 17.00 0 0.81 0.377339 0.86
675 1035 3k8z (B) 65 360 30.00 0.000047 1 0.263037 0.96
815 1036 5ijz (A) 196 383 28.00 0.12 1 0.23378 0.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_130284)

Species Accession Gene Product
Babesia bovis BBOV_III009680   Glutamate/Leucine/Phenylalanine/Valine dehydrogenase family protein
Candida albicans CaO19.9738   NAD-glutamate dehydrogenase
Candida albicans CaO19.2192   NAD-glutamate dehydrogenase
Dictyostelium discoideum DDB_G0280319   NAD+ dependent glutamate dehydrogenase
Entamoeba histolytica EHI_075150   NAD-specific glutamate dehydrogenase, putative
Leishmania braziliensis LbrM.15.1050   glutamate dehydrogenase
Leishmania donovani LdBPK_151070.1   glutamate dehydrogenase
Leishmania infantum LinJ.15.1070   glutamate dehydrogenase
Leishmania major LmjF.15.1010   glutamate dehydrogenase
Leishmania mexicana LmxM.15.1010   glutamate dehydrogenase
Neospora caninum NCLIV_065590   NAD-specific glutamate dehydrogenase, putative
Plasmodium berghei PBANKA_1228200   glutamate dehydrogenase, putative
Plasmodium falciparum PF3D7_0802000   glutamate dehydrogenase, putative
Plasmodium knowlesi PKNH_0116900   glutamate dehydrogenase, putative
Plasmodium vivax PVX_093640   NAD-specific glutamate dehydrogenase, putative
Plasmodium yoelii PY03701   glutamate dehydrogenase-related
Saccharomyces cerevisiae YDL215C   glutamate dehydrogenase (NAD(+))
Trypanosoma brucei gambiense Tbg972.9.3100   glutamate dehydrogenase, putative
Trypanosoma brucei Tb927.9.5900   glutamate dehydrogenase
Trypanosoma congolense TcIL3000_9_1990   glutamate dehydrogenase
Trypanosoma cruzi TcCLB.509445.39   glutamate dehydrogenase, putative
Trypanosoma cruzi TcCLB.505843.10   glutamate dehydrogenase, putative
Toxoplasma gondii TGME49_249390   glutamate/leucine/phenylalanine/valine dehydrogenase family protein
Theileria parva TP04_0883   NAD-specific glutamate dehydrogenase, putative

Essentiality

TGME49_249390 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.4310 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.4310 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.4310 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.4310 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1228200 Plasmodium berghei Dispensable plasmo
TGME49_249390 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TGME49_249390 (Toxoplasma gondii), glutamate/leucine/phenylalanine/valine dehydrogenase family protein
Title for this comment
Comment