Detailed view for PF3D7_1426700

Basic information

TDR Targets ID: 2577
Plasmodium falciparum, phosphoenolpyruvate carboxylase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 7.8485 | Length (AA): 1148 | MW (Da): 133960 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00311   Phosphoenolpyruvate carboxylase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0008964   phosphoenolpyruvate carboxylase activity  
GO:0003824   catalytic activity  
GO:0006099   tricarboxylic acid cycle  
GO:0015977   carbon utilization by fixation of carbon dioxide  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
47 991 1jqo (A) 35 947 36.00 0 1 1.33 -1.4
50 949 1jqn (A) 5 852 35.00 0 1 1.32 -1.69
22 979 3zgb (A) 6 964 35.00 0 1 1.32839 -1.01
22 1007 3zgb (A) 6 965 35.00 0 1 1.31879 -0.97
56 1007 1jqo (A) 38 969 36.00 0 1 1.27417 -0.72
993 1144 4uos (A) 1 155 10.00 0.18 0.05 0.318004 -1.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Ring, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, late schizont, Oocyst. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile gametocyte, sporozoite, early ring, early schizont, Sporozoite. PlasmoDB Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_130145)

Species Accession Gene Product
Arabidopsis thaliana AT2G42600   phosphoenolpyruvate carboxylase 2
Arabidopsis thaliana AT3G14940   phosphoenolpyruvate carboxylase 3
Arabidopsis thaliana AT1G53310   phosphoenolpyruvate carboxylase 1
Arabidopsis thaliana AT3G42628   phosphoenolpyruvate carboxylase-related protein
Babesia bovis BBOV_II002090   phosphoenolpyruvate carboxylase, putative
Cryptosporidium hominis Chro.50389   phosphoenolpyruvate carboxylase
Cryptosporidium parvum cgd5_70   phosphoenolpyruvate carboxylase
Dictyostelium discoideum DDB_G0287723   hypothetical protein
Escherichia coli b3956   phosphoenolpyruvate carboxylase
Mycobacterium leprae ML0578   Probable phosphoenolpyruvate carboxylase Ppc
Mycobacterium ulcerans MUL_1836   phosphoenolpyruvate carboxylase
Oryza sativa 4328859   Os02g0244700
Oryza sativa 4325309   Os01g0758300
Oryza sativa 4325531   Os01g0208700
Oryza sativa 4345387   Os08g0366000
Oryza sativa 4346699   Os09g0315700
Plasmodium berghei PBANKA_1017900   phosphoenolpyruvate carboxylase
Plasmodium falciparum PF3D7_1426700   phosphoenolpyruvate carboxylase
Plasmodium knowlesi PKNH_1332000   phosphoenolpyruvate carboxylase, putative
Plasmodium yoelii PY00206   phosphoenolpyruvate carboxylase

Essentiality

PF3D7_1426700 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b3956 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Phospho(Enol)Pyruvate Carboxylase (4.1.1.31 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Plasmodium berghei ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    PF3D7_1426700 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Plasmodium berghei ( 1 )

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1426700 (Plasmodium falciparum), phosphoenolpyruvate carboxylase
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