Detailed view for TGME49_208490

Basic information

TDR Targets ID: 257768
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 4.8331 | Length (AA): 1064 | MW (Da): 118278 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01926   50S ribosome-binding GTPase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  

Metabolic Pathways

Ribosome biogenesis in eukaryotes (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
32 222 5cwn (A) 7 200 16.00 0.017 0.04 0.345311 -0.67
311 389 5hmo (A) 812 890 33.00 0.0025 0.02 0.550948 -1.72
651 718 3geh (A) 199 275 44.00 0.16 0.99 0.43861 0.27
669 721 3a1s (A) 19 72 47.00 0.29 0.84 0.538512 0.08
671 712 4dcu (A) 180 230 50.00 0.29 0.58 0.493174 0.27

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127871)

Species Accession Gene Product
Arabidopsis thaliana AT1G08410   P-loop containing nucleoside triphosphate hydrolases superfamily protein
Arabidopsis thaliana AT2G27200   P-loop containing nucleoside triphosphate hydrolase-like protein
Babesia bovis BBOV_IV010460   GTPase subfamily protein
Brugia malayi Bm1_28980   hypothetical protein
Candida albicans CaO19.10967   growth, morphology, mating, sporulation
Candida albicans CaO19.3463   growth, morphology, mating, sporulation
Caenorhabditis elegans CELE_C53H9.2   Protein C53H9.2, isoform C
Cryptosporidium hominis Chro.20436   GTP-binding protein
Cryptosporidium parvum cgd2_4090   YawG/Kre35p-like, Yjeq GTpase
Dictyostelium discoideum DDB_G0294292   unclassified GTPase
Drosophila melanogaster Dmel_CG14788   Nucleostemin ortholog (H. sapiens) 3
Echinococcus granulosus EgrG_000219400   GTP binding protein HSR1
Entamoeba histolytica EHI_118800   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000219400   GTP binding protein, HSR1
Giardia lamblia GL50803_15867   GTP-binding protein
Homo sapiens ENSG00000041802   large 60S subunit nuclear export GTPase 1
Leishmania braziliensis LbrM.05.0200   hypothetical protein, conserved
Leishmania donovani LdBPK_050200.1   50S ribosome-binding GTPase, putative
Leishmania infantum LinJ.05.0200   hypothetical protein, conserved
Leishmania major LmjF.05.0200   hypothetical protein, conserved
Leishmania mexicana LmxM.05.0200   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_08056   hypothetical protein
Mus musculus 224092   large subunit GTPase 1 homolog (S. cerevisiae)
Neospora caninum NCLIV_003130   GTP-binding protein, related
Oryza sativa 4333574   Os03g0647500
Oryza sativa 4352809   Os12g0618300
Onchocerca volvulus OVOC73   Large subunit GTPase 1 homolog
Plasmodium berghei PBANKA_1013500   large subunit GTPase 1, putative
Plasmodium falciparum PF3D7_1431300   large subunit GTPase 1, putative
Plasmodium knowlesi PKNH_1327400   large subunit GTPase 1, putative
Plasmodium vivax PVX_084975   hypothetical protein, conserved
Plasmodium yoelii PY06180   unnamed protein product
Saccharomyces cerevisiae YGL099W   putative GTPase LSG1
Schistosoma japonicum Sjp_0021210   ko:K06948 GTP-binding protein MMR1, putative
Schistosoma mansoni Smp_147850   GTP-binding protein-related
Schmidtea mediterranea mk4.006673.01   GTP-binding protein-related
Schmidtea mediterranea mk4.006673.02  
Schmidtea mediterranea mk4.008284.01  
Trypanosoma brucei gambiense Tbg972.5.5820   GTP-binding protein, putative
Trypanosoma brucei Tb927.5.4310   GTP-binding protein, putative
Trypanosoma congolense TcIL3000_5_4930   GTP-binding protein, putative
Trypanosoma cruzi TcCLB.510351.70   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.507601.90   hypothetical protein, conserved
Toxoplasma gondii TGME49_208490   hypothetical protein
Theileria parva TP01_0681   hypothetical protein, conserved
Trichomonas vaginalis TVAG_248980   mmr1/hsr1 GTP binding protein, putative

Essentiality

TGME49_208490 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.4310 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.4310 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.4310 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.4310 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C53H9.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_C53H9.2 Caenorhabditis elegans larval arrest wormbase
CELE_C53H9.2 Caenorhabditis elegans slow growth wormbase
CELE_C53H9.2 Caenorhabditis elegans sterile wormbase
YGL099W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1013500 Plasmodium berghei Essential plasmo
TGME49_208490 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_208490 (Toxoplasma gondii), hypothetical protein
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