Detailed view for TGME49_220840

Basic information

TDR Targets ID: 257817
Toxoplasma gondii, threonine synthase
Source Database / ID:  ToxoDB 

pI: 7.8725 | Length (AA): 571 | MW (Da): 62984 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0030170   pyridoxal phosphate binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 538 1kl7 (A) 8 511 28.00 0 1 0.9 0.42
2 550 4f4f (A) 86 463 35.00 0 1 0.877471 0.72
7 311 3v7n (A) 117 339 42.00 0 1 0.430251 1.32
422 466 2rhg (B) 326 370 24.00 0 0.47 0.417009 -0.72

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Tachyzoite. Gregory
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128662)

Species Accession Gene Product
Candida albicans CaO19.4233   threonine synthase
Candida albicans CaO19.11708   threonine synthase
Escherichia coli b0004   L-threonine synthase
Homo sapiens ENSG00000185875   threonine synthase-like 1 (S. cerevisiae)
Homo sapiens ENSG00000144115   threonine synthase-like 2 (S. cerevisiae)
Leishmania braziliensis LbrM.14.0350   threonine synthase, putative
Leishmania donovani LdBPK_140350.1   threonine synthase, putative
Leishmania infantum LinJ.14.0350   threonine synthase, putative
Leishmania major LmjF.14.0350   threonine synthase, putative
Leishmania mexicana LmxM.14.0350   threonine synthase, putative
Mus musculus ENSMUSG00000048550   threonine synthase-like 1 (bacterial)
Mus musculus ENSMUSG00000054474   threonine synthase-like 2 (bacterial)
Neospora caninum NCLIV_004630   threonine synthase, putative
Saccharomyces cerevisiae YCR053W   threonine synthase THR4
Schmidtea mediterranea mk4.002839.00  
Schmidtea mediterranea mk4.044997.02   Threonine synthase-like 1
Schmidtea mediterranea mk4.000631.16  
Schmidtea mediterranea mk4.007220.00  
Schmidtea mediterranea mk4.003415.00  
Trypanosoma brucei gambiense Tbg972.7.4960   threonine synthase, putative
Trypanosoma brucei Tb927.7.4390   threonine synthase, putative
Trypanosoma congolense TcIL3000_7_3590   threonine synthase, putative
Trypanosoma cruzi TcCLB.504213.80   threonine synthase, putative
Trypanosoma cruzi TcCLB.508857.160   threonine synthase, putative
Toxoplasma gondii TGME49_220840   threonine synthase

Essentiality

TGME49_220840 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.4390 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.4390 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.4390 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.4390 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0004 Escherichia coli non-essential goodall
TGME49_220840 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_220840 (Toxoplasma gondii), threonine synthase
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