Detailed view for LmjF.36.6400

Basic information

TDR Targets ID: 25795
Leishmania major, 40S ribosomal protein S8, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 11.0285 | Length (AA): 260 | MW (Da): 29766 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01201   Ribosomal protein S8e

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 80 2nca (A) 38 110 34.00 0.19 0.02 0.709008 -1.19
7 167 4uos (A) 20 173 25.00 0.062 0.86 1.05953 -1.61
167 260 2mvf (A) 168 261 72.00 0 1 1.16124 0.16

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128061)

Species Accession Gene Product
Arabidopsis thaliana AT5G06360   ribosomal protein S8e family protein
Babesia bovis BBOV_III000260   ribosomal protein Se8, putative
Brugia malayi Bm1_51110   TGF beta-inducible nuclear protein 1
Candida albicans CaO19.7424   similar to S. cerevisiae NSA2 (YER126C) Nop7 Associated Protein 2 involved in ribosomal large subunit maturation
Candida albicans CaO19.7398   similar to C terminus of S. cerevisiae NSA2 (YER126C) involved in ribosomal large subunit maturation
Caenorhabditis elegans CELE_W09C5.1   Protein W09C5.1
Cryptosporidium hominis Chro.30461   RIKEN cDNA 5730427N09 gene
Cryptosporidium parvum cgd3_4100   conserved protein, COG SSU ribosomal protein S8E
Dictyostelium discoideum DDB_G0290031   hypothetical protein
Drosophila melanogaster Dmel_CG5277   Intronic Protein 259
Echinococcus granulosus EgrG_000478000   TGF beta inducible nuclear protein 1
Entamoeba histolytica EHI_099760   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000478000   TGF beta inducible nuclear protein 1
Giardia lamblia GL50803_11755   Hypothetical protein
Homo sapiens ENSG00000164346   NSA2 ribosome biogenesis homolog (S. cerevisiae)
Leishmania braziliensis LbrM.35.6730   40S ribosomal protein S8, putative
Leishmania donovani LdBPK_366680.1   40S ribosomal protein S8, putative
Leishmania infantum LinJ.36.6680   40S ribosomal protein S8, putative
Leishmania major LmjF.36.6400   40S ribosomal protein S8, putative
Leishmania mexicana LmxM.36.6400   40S ribosomal protein S8, putative
Loa Loa (eye worm) LOAG_09193   TGF beta-inducible nuclear protein 1
Mus musculus ENSMUSG00000060739   NSA2 ribosome biogenesis homolog (S. cerevisiae)
Mus musculus 101055813   predicted gene, 29726
Mus musculus 102642514   ribosome biogenesis protein NSA2 homolog
Neospora caninum NCLIV_017790   TGF-beta-inducible nuclear protein 1, putative
Oryza sativa 4344253   Os07g0673100
Onchocerca volvulus OVOC11911   Ribosome biogenesis protein NSA2 homolog
Plasmodium berghei PBANKA_0805100   ribosomal protein S8e, putative
Plasmodium falciparum PF3D7_0707900   ribosomal protein S8e, putative
Plasmodium knowlesi PKNH_0106400   ribosomal protein S8e, putative
Plasmodium vivax PVX_087920   ribosomal protein S8e, putative
Plasmodium yoelii PY00137   hairy cell leukemia protein 1
Saccharomyces cerevisiae YER126C   Nsa2p
Schistosoma japonicum Sjp_0211090   Ribosome biogenesis protein NSA2 homolog, putative
Schistosoma japonicum Sjp_0133960   Ribosome biogenesis protein NSA2 homolog, putative
Schistosoma mansoni Smp_054120   Ribosome biogenesis protein NSA2 homologue
Schmidtea mediterranea mk4.006414.02   Ribosome biogenesis protein NSA2 homologue
Trypanosoma brucei gambiense Tbg972.10.9450   40S ribosomal protein S8, putative
Trypanosoma brucei Tb927.10.7710   40S ribosomal protein S8, putative
Trypanosoma cruzi TcCLB.506249.60   40S ribosomal protein S8, putative
Trypanosoma cruzi TcCLB.508231.200   40S ribosomal protein S8, putative
Toxoplasma gondii TGME49_242800   ribosome biogenesis protein NSA2, putative
Theileria parva TP03_0821   hypothetical protein, conserved
Trichomonas vaginalis TVAG_192990   TGF-beta-inducible nuclear protein, putative
Trichomonas vaginalis TVAG_247360   TGF-beta-inducible nuclear protein, putative

Essentiality

LmjF.36.6400 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.7710 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.7710 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.7710 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.7710 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_W09C5.1 Caenorhabditis elegans slow growth wormbase
YER126C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0805100 Plasmodium berghei Slow plasmo
TGME49_242800 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.36.6400 (Leishmania major), 40S ribosomal protein S8, putative
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