Detailed view for TGME49_211050

Basic information

TDR Targets ID: 257959
Toxoplasma gondii, ATPase, AAA family protein
Source Database / ID:  ToxoDB 

pI: 9.7808 | Length (AA): 791 | MW (Da): 89915 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)

Gene Ontology

Mouse over links to read term descriptions.
GO:0017111   nucleoside-triphosphatase activity  
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0000166   nucleotide binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
533 811 1lv7 (A) 144 398 21.00 0 1 0.53 -0.41
533 855 2ce7 (A) 150 442 24.00 0 1 0.53 -0.17
576 697 1e32 (A) 237 352 36.00 0.000000000023 1 0.62 -0.82
489 753 2zam (A) 143 381 30.00 0.000000000012 1 0.429819 1.19
498 680 1d2n (A) 531 688 32.00 0.021 0.99 0.516153 -0.42
512 574 1hqc (A) 37 109 41.00 0.21 0.96 0.334446 0.95
512 633 5c18 (A) 237 352 36.00 0.0000000001 1 0.588035 -0.4

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_129551)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG16789   CG16789 gene product from transcript CG16789-RA
Drosophila melanogaster Dmel_CG14183   CG14183 gene product from transcript CG14183-RA
Echinococcus granulosus EgrG_001013100   IQ and AAA domain containing protein 1
Echinococcus multilocularis EmuJ_001013100   IQ and AAA domain containing protein 1
Giardia lamblia GL50803_94322   Spastin
Homo sapiens ENSG00000132321   IQ motif containing with AAA domain 1
Homo sapiens 102723607   putative IQ and AAA domain-containing protein 1-like
Leishmania braziliensis LbrM.13.1280   hypothetical protein, conserved
Leishmania donovani LdBPK_131580.1   ATPase family associated with various cellular activities (AAA), putative
Leishmania infantum LinJ.13.1580   hypothetical protein, conserved
Leishmania major LmjF.13.1520   hypothetical protein, conserved
Leishmania mexicana LmxM.13.1520   hypothetical protein, conserved
Mus musculus ENSMUSG00000026301   IQ motif containing with AAA domain
Mus musculus ENSMUSG00000038199   RIKEN cDNA 4931409K22 gene
Neospora caninum NCLIV_011810   AAA family ATPase domain-containing protein, putative
Schistosoma japonicum Sjp_0006740   IQ and AAA domain-containing protein, putative
Schistosoma japonicum Sjp_0006750   IQ and AAA domain-containing protein, putative
Schistosoma mansoni Smp_013080   hypothetical protein
Schmidtea mediterranea mk4.000317.00  
Trypanosoma brucei gambiense Tbg972.11.3800   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.3360   Component of motile flagella 22
Trypanosoma congolense TcIL3000_0_10780   ATPase family associated with various cellular activities (AAA), putative
Trypanosoma congolense TcIL3000.11.3230   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.507099.10   ATPase family associated with various cellular activities (AAA), putative
Trypanosoma cruzi TcCLB.503999.110   ATPase family associated with various cellular activities (AAA), putative
Toxoplasma gondii TGME49_211050   ATPase, AAA family protein
Trichomonas vaginalis TVAG_493270   conserved hypothetical protein

Essentiality

TGME49_211050 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.0860 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.0860 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.0860 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.0860 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_211050 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_211050 (Toxoplasma gondii), ATPase, AAA family protein
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