Detailed view for TGME49_265380

Basic information

TDR Targets ID: 257960
Toxoplasma gondii, tetratricopeptide repeat (TPR)-/ U-box domain-containing protein
Source Database / ID:  ToxoDB 

pI: 6.6298 | Length (AA): 261 | MW (Da): 29432 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04564   U-box domain
PF12895   Anaphase-promoting complex, cyclosome, subunit 3

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  
GO:0005488   binding  
GO:0004842   ubiquitin-protein ligase activity  
GO:0016567   protein ubiquitination  

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 120 1elw (A) 2 118 23.00 0 1 0.94 -1.48
5 242 2c2l (A) 26 290 28.00 0 1 1.17 0.61
2 100 2vyi (A) 87 185 38.00 0.00000000028 1 0.97331 -1.21
2 31 1w3b (A) 75 104 30.00 0.15 0.53 0.470743 -0.31
6 259 2c2l (A) 27 302 33.00 0 1 1.24508 0.61
6 257 2c2l (A) 27 300 37.00 0 1 1.29552 0.53
157 240 4wz3 (B) 9 85 39.00 0.000047 0.97 0.712839 -0.01

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_129825)

Species Accession Gene Product
Arabidopsis thaliana AT3G07370   E3 ubiquitin-protein ligase CHIP
Babesia bovis BBOV_IV004490   tetratricopeptide repeat (TPR)-/ U-box domain-containing protein
Brugia malayi Bm1_32440   RE01069p
Candida albicans CaO19.14154   similar to carboxy terminus of Hsp70-interacting protein
Candida albicans CaO19.6864   similar to carboxy terminus of Hsp70-interacting protein
Caenorhabditis elegans CELE_T09B4.10   Protein CHN-1
Drosophila melanogaster Dmel_CG5203   CG5203 gene product from transcript CG5203-RA
Homo sapiens ENSG00000103266   STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
Loa Loa (eye worm) LOAG_08950   hypothetical protein
Mus musculus ENSMUSG00000039615   STIP1 homology and U-Box containing protein 1
Neospora caninum NCLIV_039890   TPR domain-containing protein, putative
Oryza sativa 4344499   Os08g0113300
Oryza sativa 4337553   Os05g0104900
Plasmodium berghei PBANKA_0804900   E3 ubiquitin-protein ligase, putative
Plasmodium falciparum PF3D7_0707700   E3 ubiquitin-protein ligase, putative
Plasmodium knowlesi PKNH_0106200   E3 ubiquitin-protein ligase, putative
Plasmodium vivax PVX_087910   E3 ubiquitin-protein ligase, putative
Plasmodium yoelii PY00139   ERYTHROCYTE MEMBRANE PROTEIN PFEMP3
Toxoplasma gondii TGME49_265380   tetratricopeptide repeat (TPR)-/ U-box domain-containing protein
Theileria parva TP01_0301   hypothetical protein, conserved
Trichomonas vaginalis TVAG_315350   chaperone binding protein, putative

Essentiality

TGME49_265380 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_T09B4.10 Caenorhabditis elegans larval lethal wormbase
PBANKA_0804900 Plasmodium berghei Slow plasmo
TGME49_265380 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Calcineurin (3.1.3.16 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

6 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_265380 (Toxoplasma gondii), tetratricopeptide repeat (TPR)-/ U-box domain-containing protein
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