Detailed view for LmjF.27.2210

Basic information

TDR Targets ID: 25798
Leishmania major, dual specificity protein phosphatase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.6546 | Length (AA): 352 | MW (Da): 38532 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00782   Dual specificity phosphatase, catalytic domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008138   protein tyrosine/serine/threonine phosphatase activity  
GO:0016791   phosphoric monoester hydrolase activity  
GO:0016311   dephosphorylation  
GO:0006470   protein amino acid dephosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
151 329 1vhr (A) 10 182 29.00 0 1 0.74 -0.96
185 320 1zzw (A) 321 458 37.00 0 1 1.03 -2.04
163 330 2y96 (A) 32 206 27.00 0 1 0.816373 -0.48
182 324 2g6z (A) 175 317 33.00 0 1 0.94035 -1.43
183 321 2r0b (A) 26 171 40.00 0.000000000006 1 0.931986 -1.26
184 320 3s4e (A) 0 200 37.00 0 1 1.0002 -1.59
185 324 4yr8 (G) 158 298 33.00 0 1 0.948827 -1.7

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128368)

Species Accession Gene Product
Arabidopsis thaliana AT3G06110   MAPK phosphatase 2
Caenorhabditis elegans CELE_Y54F10BM.13   Protein Y54F10BM.13
Cryptosporidium hominis Chro.30177   dual specificity protein phosphatase
Cryptosporidium parvum cgd3_1470   dual specificity phosphatase
Dictyostelium discoideum DDB_G0283417   hypothetical protein
Drosophila melanogaster Dmel_CG34099   MAP kinase-specific phosphatase
Echinococcus granulosus EgrG_001042600   dual specificity protein phosphatase 19
Entamoeba histolytica EHI_053090   leucine rich repeat and phosphatase domain containing protein
Entamoeba histolytica EHI_110140   dual specificity protein phosphatase, putative
Echinococcus multilocularis EmuJ_001042600   dual specificity protein phosphatase 19
Homo sapiens ENSG00000162999   dual specificity phosphatase 19
Homo sapiens ENSG00000198252   serine/threonine/tyrosine interacting protein
Leishmania braziliensis LbrM.27.2390   dual specificity protein phosphatase, putative
Leishmania donovani LdBPK_272130.1   dual specificity protein phosphatase, putative
Leishmania infantum LinJ.27.2130   dual specificity protein phosphatase, putative
Leishmania major LmjF.27.2210   dual specificity protein phosphatase, putative
Leishmania mexicana LmxM.27.2210   dual specificity protein phosphatase, putative
Loa Loa (eye worm) LOAG_06386   hypothetical protein
Mus musculus ENSMUSG00000027001   dual specificity phosphatase 19
Mus musculus 56291   serine/threonine/tyrosine interaction protein
Onchocerca volvulus OVOC7776   Dual specificity protein phosphatase 19 homolog
Plasmodium yoelii PY00863   hypothetical protein
Schistosoma japonicum Sjp_0305590   ko:K01090 protein phosphatase [EC3.1.3.16], putative
Schistosoma mansoni Smp_084930   dual specificity protein phosphatase
Schmidtea mediterranea mk4.000367.01   Putative dual specificity protein phosphatase
Trypanosoma brucei gambiense Tbg.972.2.2900   dual specificity protein phosphatase, putative
Trypanosoma brucei Tb927.2.4870   kinetoplastid-specific dual specificity phosphatase, putative
Trypanosoma cruzi TcCLB.511621.190   dual specificity protein phosphatase, putative
Trypanosoma cruzi TcCLB.504741.170   dual specificity protein phosphatase, putative
Trichomonas vaginalis TVAG_106820   vh3 dual specificity phosphatase, putative
Trichomonas vaginalis TVAG_419930   dual specificity protein phosphatase, putative
Trichomonas vaginalis TVAG_323340   vh3 dual specificity phosphatase, putative
Trichomonas vaginalis TVAG_160370   vh5 dual specificity phosphatase, putative

Essentiality

LmjF.27.2210 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.4870 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.4870 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.2.4870 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.2.4870 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LmjF.27.2210 (Leishmania major), dual specificity protein phosphatase, putative
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