Detailed view for TGME49_225490

Basic information

TDR Targets ID: 258022
Toxoplasma gondii, calcium-dependent protein kinase CDPK2
Source Database / ID:  ToxoDB 

pI: 6.7551 | Length (AA): 711 | MW (Da): 79134 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00686   Starch binding domain
PF13499   EF-hand domain pair

Gene Ontology

Mouse over links to read term descriptions.
GO:2001070   GO:starch binding  

GO:0030246   carbohydrate binding  
GO:0005524   ATP binding  
GO:0005509   calcium ion binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0003824   catalytic activity  
GO:0006468   protein amino acid phosphorylation  
GO:0005975   carbohydrate metabolic process  

Metabolic Pathways

Toxoplasmosis (KEGG)

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 104 1cyg () 580 680 21.00 0 0.43 0.39 -0.71
108 592 2acx (A) 14 532 18.00 0 1 0.59 0.23
234 495 1yrp (A) 7 276 38.00 0 1 0.94 -1.58
1 103 1pam (A) 585 684 21.00 0 0.79 0.371666 -0.71
2 103 1cgt (A) 584 683 20.00 0 1 0.35826 -0.59
13 105 2vn4 (A) 506 599 32.00 0 0.99 0.403602 -0.16
73 551 3pfq (A) 157 657 22.00 0 1 0.788299 0.64
117 513 3i6u (A) 95 501 28.00 0 0.94 0.801169 0.19
170 681 3v5w (A) 121 665 21.00 0 1 0.804713 0.84
228 497 2qg5 (A) 16 285 68.00 0 1 1.24535 -1.07
538 602 5d67 (A) 1024 1087 22.00 0.22 0.88 0.554021 -1.86

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_170347)

Species Accession Gene Product
Cryptosporidium hominis Chro.70214   calmodulin-domain protein kinase 2
Cryptosporidium parvum cgd7_1840   calcium/calmodulin-dependent protein kinase with a kinase domain and 4 calmodulin like EF hands
Neospora caninum NCLIV_047220   Calcium-dependent protein kinase, related
Toxoplasma gondii TGME49_225490   calcium-dependent protein kinase CDPK2

Essentiality

TGME49_225490 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_225490 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 32.3% 291 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 29.2% 298 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 29.6% 291 aa Compounds References
Bos taurus Calmodulin 149 aa 36.7% 147 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 23.3% 301 aa Compounds References
Patiria pectinifera Cdc2 300 aa 30.3% 287 aa Compounds References
Plasmodium falciparum (isolate 3D7) Calcium-dependent protein kinase 4 528 aa 36.9% 480 aa Compounds References
Zea mays CaM kinase I alpha 492 aa 40.0% 457 aa Compounds References
Triticum aestivum Calcium dependent protein kinase 548 aa 38.8% 451 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 28.7% 300 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 29.3% 311 aa Compounds References
Bos taurus Glycogen synthase kinase-3 beta splice variant X1 419 aa 29.8% 336 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 32.7% 294 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0033 1 1
0.0039 0.9485 0.5
0.0008 0.5 0.5
0.0022 0.5 0.5
0.0036 0.5 0.5
0.0081 0.5 0.5
0.0059 1 1
0.0004 0.5 0.5
0.0042 0.5 0.5
0.0067 0.5 0.5
0.0039 0.5 0.5
0.0081 1 0.5
0.0066 0.3101 0
0.0032 0.5 0.5
0.0012 0.5 0.5
0.0027 1 0.5
0.0069 0.3067 1
0.0026 0.5 0.5
0.0091 1 0.5
0.0003 0.5 0.5
0.0092 1 0.5
0.0007 0.5 0.5
0.0039 0.5 0.5
0.0063 0.7244 0.7244
0.0061 0.6883 0.6656
0.0093 0.8828 0
0.0029 0.5 0.5
0.0037 1 0.5
0.0018 0.5 0.5
0.0062 0.6935 0
0.0023 0.5 0.5
0.0011 1 0.5
0.0007 0.5 0.5
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0059 1 1
0.0064 0.3377 0
0.0016 0.5 0.5
0.0059 1 1
0.0032 0.5 0.5
0.0098 0.3242 0.2614
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0056 1 0.5
0.0088 0.4477 1
0.0063 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

12 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_225490 (Toxoplasma gondii), calcium-dependent protein kinase CDPK2
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