Detailed view for TGME49_221420

Basic information

TDR Targets ID: 258136
Toxoplasma gondii, Chromosome-associated kinesin KLP1, putative
Source Database / ID:  ToxoDB 

pI: 6.5208 | Length (AA): 928 | MW (Da): 104258 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00225   Kinesin motor domain
PF00498   FHA domain
PF12423   Kinesin protein 1B

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0003777   microtubule motor activity  
GO:0008017   microtubule binding  
GO:0007018   microtubule-based movement  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 406 4frz (A) 886 1252 34.00 0 1 0.6832 -0.08
4 363 1vfv (A) 5 361 54.00 0 1 0.905631 -0.47
5 354 4ozq (A) 394 734 51.00 0 1 0.945855 -0.68
82 352 4etp (A) 451 719 43.00 0 1 0.804726 -0.82
280 386 3kin (B) 256 361 47.00 0 1 0.526002 0.09
368 659 5cwm (A) 7 227 33.00 0.0026 1 0.397355 0.18
443 559 1wln (A) 4 115 29.00 0 0.98 0.399778 0.07
586 674 4mh6 (A) 0 85 18.00 0 0.01 0.414605 -2

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_126833)

Species Accession Gene Product
Brugia malayi Bm1_02450   kinesin like protein KLP-4
Brugia malayi Bm1_42245   kinesin-related protein
Brugia malayi Bm1_42240   Uncoordinated protein 104
Brugia malayi Bm1_42250   Uncoordinated protein 104
Candida albicans CaO19.3667   kinesin motor domain protein
Candida albicans CaO19.11151   kinesin motor domain protein
Caenorhabditis elegans CELE_R144.1   Protein KLP-6
Caenorhabditis elegans CELE_C52E12.2   Protein UNC-104, isoform B
Caenorhabditis elegans CELE_F56E3.3   Protein KLP-4, isoform A
Dictyostelium discoideum DDB_G0290963   kinesin family member 1
Drosophila melanogaster Dmel_CG8566   unc-104 ortholog (C. elegans)
Drosophila melanogaster Dmel_CG8183   Kinesin heavy chain 73
Echinococcus granulosus EgrG_000732900   kinesin
Echinococcus granulosus EgrG_000626900   Kinesin motor region
Echinococcus granulosus EgrG_000518900   kinesin protein KIF1B
Echinococcus multilocularis EmuJ_000732900   kinesin
Echinococcus multilocularis EmuJ_000518900   kinesin protein KIF1B
Echinococcus multilocularis EmuJ_000626900   Kinesin, motor region
Giardia lamblia GL50803_112846   Kinesin-3
Giardia lamblia GL50803_6262   Kinesin-3
Giardia lamblia GL50803_102101   Kinesin-3
Homo sapiens ENSG00000137177   kinesin family member 13A
Homo sapiens ENSG00000197892   kinesin family member 13B
Homo sapiens ENSG00000054523   kinesin family member 1B
Homo sapiens ENSG00000130294   kinesin family member 1A
Homo sapiens ENSG00000129250   kinesin family member 1C
Leishmania braziliensis LbrM.20.3890   Unc104-like kinesin, putative
Leishmania infantum LinJ.34.4090   Unc104-like kinesin, putative
Leishmania major LmjF.34.4260   Unc104-like kinesin, putative
Leishmania mexicana LmxM.33.4260   Unc104-like kinesin, putative
Loa Loa (eye worm) LOAG_15064   hypothetical protein
Loa Loa (eye worm) LOAG_10299   hypothetical protein
Loa Loa (eye worm) LOAG_15935   hypothetical protein
Loa Loa (eye worm) LOAG_10397   KIF16B
Loa Loa (eye worm) LOAG_14247   hypothetical protein
Loa Loa (eye worm) LOAG_07928   Kif1c protein
Loa Loa (eye worm) LOAG_13846   hypothetical protein
Loa Loa (eye worm) LOAG_13824   hypothetical protein
Loa Loa (eye worm) LOAG_07927   uncoordinated protein 104
Mus musculus ENSMUSG00000014602   kinesin family member 1A
Mus musculus ENSMUSG00000063077   kinesin family member 1B
Mus musculus ENSMUSG00000020821   kinesin family member 1C
Mus musculus ENSMUSG00000021375   kinesin family member 13A
Mus musculus ENSMUSG00000087236   predicted gene 1305
Mus musculus ENSMUSG00000060012   kinesin family member 13B
Neospora caninum NCLIV_004960   kinesin motor domain cointaining protein, putative
Neospora caninum NCLIV_062040   kinesin, putative
Schistosoma japonicum Sjp_0009810   ko:K10392 kinesin family member 1/13/14, putative
Schistosoma japonicum Sjp_0015350   Kinesin-like protein KIF1A, putative
Schistosoma japonicum Sjp_0123640   Kinesin-like protein unc-104, putative
Schistosoma japonicum Sjp_0009820   Kinesin-like protein KIF1B, putative
Schistosoma mansoni Smp_159790   hypothetical protein
Schistosoma mansoni Smp_134330   hypothetical protein
Schmidtea mediterranea mk4.000503.08  
Schmidtea mediterranea mk4.010388.00  
Schmidtea mediterranea mk4.000503.11   Kinesin-73
Schmidtea mediterranea mk4.000503.13   Kinesin-73
Schmidtea mediterranea mk4.013742.00  
Schmidtea mediterranea mk4.002945.12   Kinesin-like protein unc-104
Schmidtea mediterranea mk4.003433.01   Kinesin-73
Schmidtea mediterranea mk4.003433.02   Kinesin-73
Schmidtea mediterranea mk4.000477.01  
Schmidtea mediterranea mk4.010653.00  
Schmidtea mediterranea mk4.002945.11   Kinesin-like protein unc-104
Schmidtea mediterranea mk4.002945.06   Kinesin-like protein unc-104
Schmidtea mediterranea mk4.013742.02   Kinesin-related
Schmidtea mediterranea mk4.003433.00  
Schmidtea mediterranea mk4.000477.02  
Schmidtea mediterranea mk4.002945.07   Kinesin-like protein unc-104
Schmidtea mediterranea mk4.000503.09  
Schmidtea mediterranea mk4.029123.00   Kinesin-like protein unc-104
Schmidtea mediterranea mk4.019823.00  
Schmidtea mediterranea mk4.002549.02  
Schmidtea mediterranea mk4.025365.00  
Schmidtea mediterranea mk4.019823.02  
Schmidtea mediterranea mk4.002945.04   Kinesin-like protein unc-104
Schmidtea mediterranea mk4.002549.01  
Trypanosoma congolense TcIL3000.11.2290   Unc104-like kinesin, putative
Trypanosoma cruzi TcCLB.510687.94   Unc104-like kinesin, putative
Trypanosoma cruzi TcCLB.510687.100   Unc104-like kinesin, putative
Toxoplasma gondii TGME49_221420   Chromosome-associated kinesin KLP1, putative
Toxoplasma gondii TGME49_218210   Chromosome-associated kinesin KLP1, putative

Essentiality

TGME49_221420 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_221420 this record Toxoplasma gondii Probably essential sidik
TGME49_218210 Toxoplasma gondii Probably essential sidik
TGME49_221420 this record Toxoplasma gondii Essentiality uncertain sidik
TGME49_218210 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_221420 (Toxoplasma gondii), Chromosome-associated kinesin KLP1, putative
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