Detailed view for LmjF.34.0570

Basic information

TDR Targets ID: 25832
Leishmania major, GTP-binding protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.3046 | Length (AA): 834 | MW (Da): 90530 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00009   Elongation factor Tu GTP binding domain
PF00679   Elongation factor G C-terminus
PF06421   GTP-binding protein LepA C-terminus

Gene Ontology

Mouse over links to read term descriptions.
GO:0005525   GTP binding  
GO:0003924   GTPase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
116 755 1n0u (A) 6 834 20.00 0 1 0.28 0.23
132 311 1ek0 (A) 8 172 18.00 0.00000000017 0.98 0.39 -0.89
88 307 4b3x (A) 31 231 34.00 0.0000009 0.93 0.388889 0.89
131 788 3cb4 (D) 4 554 37.00 0 1 0.880069 0.9
132 162 5fg3 (A) 17 47 45.00 0 0.25 0.40227 0.75
220 330 1t9h (A) 75 186 25.00 0.97 0.28 0.409194 -0.47
386 463 1d1n (A) 154 232 18.00 0 0.42 0.217625 -0.03

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127284)

Species Accession Gene Product
Arabidopsis thaliana AT5G39900   Small GTP-binding protein
Arabidopsis thaliana AT5G08650   small GTP-binding protein
Babesia bovis BBOV_III011750   GTP-binding protein LepA family protein
Brugia malayi Bm1_49530   Hypothetical GTP-binding protein ZK1236.1 in chromosome III
Candida albicans CaO19.5483   similar to E. coli EF-type GTP-binding protein LepA
Candida albicans CaO19.12938   similar to E. coli EF-type GTP-binding protein LepA
Caenorhabditis elegans CELE_ZK1236.1   Protein ZK1236.1
Chlamydia trachomatis CT_064   elongation factor EF-4
Dictyostelium discoideum DDB_G0291708   GTP-binding protein
Drosophila melanogaster Dmel_CG1410   waclaw
Escherichia coli b2569   back-translocating elongation factor EF4, GTPase
Homo sapiens ENSG00000151806   GUF1 GTPase homolog (S. cerevisiae)
Leishmania braziliensis LbrM.20.0500   GTP-binding protein, putative
Leishmania donovani LdBPK_340590.1   Translation factor GUF1, mitochondrial, putative
Leishmania infantum LinJ.34.0590   GTP-binding protein, putative
Leishmania major LmjF.34.0570   GTP-binding protein, putative
Leishmania mexicana LmxM.33.0570   GTP-binding protein, putative
Loa Loa (eye worm) LOAG_11093   hypothetical protein
Mycobacterium leprae ML0611   Probable GTP-binding protein LepA
Mus musculus ENSMUSG00000029208   GUF1 GTPase homolog (S. cerevisiae)
Mycobacterium tuberculosis Rv2404c   Probable GTP-binding protein LepA (GTP-binding elongation factor)
Mycobacterium ulcerans MUL_3667   GTP-binding protein LepA
Neospora caninum NCLIV_060620   GTP-binding protein LepA, related
Oryza sativa 4340102   Os06g0144800
Onchocerca volvulus OVOC13425  
Plasmodium berghei PBANKA_0812800   GTP-binding protein, putative
Plasmodium falciparum PF3D7_0911700   GTP-binding protein, putative
Plasmodium knowlesi PKNH_0709700   GTP-binding protein, putative
Plasmodium vivax PVX_099025   GTP-binding protein, putative
Plasmodium yoelii PY03426   Elongation factor Tu family, putative
Saccharomyces cerevisiae YLR289W   GTPase GUF1
Trypanosoma brucei gambiense Tbg972.10.3700   GTP-binding protein, putative,elongation factor, putative
Trypanosoma brucei Tb927.10.2960   Translation factor GUF1, mitochondrial, putative
Trypanosoma cruzi TcCLB.504105.210   Translation factor GUF1, mitochondrial, putative
Trypanosoma cruzi TcCLB.503697.90   Translation factor GUF1, mitochondrial, putative
Toxoplasma gondii TGME49_307980   GTP-binding protein lepA, putative
Treponema pallidum TP0510   GTP-binding protein LepA
Theileria parva TP01_1045   GTP-binding protein lepA, putative
Wolbachia endosymbiont of Brugia malayi Wbm0667   GTP-binding protein LepA

Essentiality

LmjF.34.0570 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.2960 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.2960 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.2960 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.10.2960 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0812800 Plasmodium berghei Slow plasmo
TGME49_307980 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.34.0570 (Leishmania major), GTP-binding protein, putative
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