Detailed view for TGME49_225070

Basic information

TDR Targets ID: 258368
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 10.1068 | Length (AA): 456 | MW (Da): 52201 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 443 1tr2 (A) 484 956 16.00 0 0.85 0.980391 0.59
25 224 2l7b (A) 71 284 30.00 0.22 0.06 0.368496 0.87
25 219 3fb2 (A) 1341 1540 12.00 0 0.08 0.413532 -0.25
272 452 4uos (A) 2 184 12.00 0.0025 0.36 0.62873 -1.48

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 merozoite, ME49 Oocyst. Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_134603)

Species Accession Gene Product
Giardia lamblia GL50803_16367   Hypothetical protein
Leishmania braziliensis LbrM.35.4510   hypothetical protein, conserved
Leishmania donovani LdBPK_364470.1   hypothetical protein, conserved
Leishmania infantum LinJ.36.4470   hypothetical protein, conserved
Leishmania major LmjF.36.4260   hypothetical protein, conserved
Leishmania mexicana LmxM.36.4260   hypothetical protein, conserved
Neospora caninum NCLIV_047640   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.10.11800   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.9640   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_10_8710   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.504147.340   hypothetical protein, conserved
Toxoplasma gondii TGME49_225070   hypothetical protein
Trichomonas vaginalis TVAG_410300   conserved hypothetical protein

Essentiality

TGME49_225070 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.9640 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.9640 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.9640 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.9640 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_225070 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_225070 (Toxoplasma gondii), hypothetical protein
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