Detailed view for TGME49_214790

Basic information

TDR Targets ID: 258709
Toxoplasma gondii, glycoprotein
Source Database / ID:  ToxoDB 

pI: 4.3944 | Length (AA): 283 | MW (Da): 31444 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02330   Mitochondrial glycoprotein

Gene Ontology

Mouse over links to read term descriptions.
GO:0005759   mitochondrial matrix  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
93 282 1p32 (A) 75 280 25.00 0 1 0.97 -0.43
128 280 1yqf (A) 44 193 23.00 0.000049 0.99 0.76 -0.53
92 282 1p32 (A) 74 280 24.00 0 1 0.958412 -0.35
97 281 3qv0 (A) 50 265 25.00 0 1 0.88461 -0.54
128 280 1yqf (A) 44 193 23.00 0.00016 0.97 0.789936 -0.7
131 233 3tt3 (L) 28 129 30.00 0.7 0.42 0.511258 0.97

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_129822)

Species Accession Gene Product
Arabidopsis thaliana AT2G39795   mitochondrial glycoprotein family protein
Arabidopsis thaliana AT3G55605   mitochondrial glycoprotein family protein
Arabidopsis thaliana AT5G02050   mitochondrial glycoprotein family protein
Arabidopsis thaliana AT2G39790   mitochondrial glycoprotein family protein
Arabidopsis thaliana AT5G05990   mitochondrial glycoprotein family protein
Babesia bovis BBOV_III002260   conserved hypothetical protein
Candida albicans CaO19.7187   Mitochondrial acidic matrix protein
Candida albicans CaO19_7187   hypothetical protein
Leishmania braziliensis LbrM.30.1090   p22 protein precursor, putative
Leishmania donovani LdBPK_301030.1   p22 protein precursor, putative
Leishmania infantum LinJ.30.1030   p22 protein precursor, putative
Leishmania major LmjF.30.0970   p22 protein precursor, putative
Leishmania mexicana LmxM.29.0970   p22 protein precursor, putative
Neospora caninum NCLIV_051840   hypothetical protein
Oryza sativa 4325458   Os01g0143300
Oryza sativa 4325461   Os01g0143700
Oryza sativa 4325462   Os01g0143800
Oryza sativa 4340910   Os06g0326500
Plasmodium berghei PBANKA_1010000   mitochondrial acidic protein MAM33, putative
Plasmodium falciparum PF3D7_1434800   mitochondrial acidic protein MAM33, putative
Plasmodium knowlesi PKNH_0421400   mitochondrial acidic protein MAM33, putative
Plasmodium vivax PVX_084795   mitochondrial acidic protein MAM33, putative
Plasmodium yoelii PY02557   putative protein, putative
Saccharomyces cerevisiae YIL070C   Mam33p
Trypanosoma brucei gambiense Tbg972.6.2180   p22 protein precursor
Trypanosoma brucei Tb927.6.2420   p22 protein precursor
Trypanosoma congolense TcIL3000_0_53490   p22 protein precursor
Trypanosoma congolense TcIL3000_6_1930   p22 protein precursor, putative
Trypanosoma cruzi TcCLB.509965.290   p22 protein precursor, putative
Trypanosoma cruzi TcCLB.509053.70   p22 protein precursor, putative
Toxoplasma gondii TGME49_214790   glycoprotein
Theileria parva TP03_0592   hypothetical protein, conserved

Essentiality

TGME49_214790 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.2420 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.2420 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.2420 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.6.2420 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_214790 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_214790 (Toxoplasma gondii), glycoprotein
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