Detailed view for TGME49_240630

Basic information

TDR Targets ID: 258738
Toxoplasma gondii, ULK kinase
Source Database / ID:  ToxoDB 

pI: 7.9568 | Length (AA): 500 | MW (Da): 55716 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
28 481 1omw (A) 189 621 21.00 0 1 0.81 0.76
173 365 1t46 (A) 668 932 21.00 0.0000000075 1 0.73 -1
18 67 3dls (A) 983 1035 30.00 0 0.22 0.2329 0.52
28 469 4cfe (C) 14 465 25.00 0 1 0.8554 0.75
29 180 4otp (A) 167 353 8.00 0 0.01 0.3779 -0.43
217 296 3vut (B) 225 303 46.00 0.022 0.02 -0.0119 2.52

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_147580)

Species Accession Gene Product
Cryptosporidium hominis Chro.70435   calcium-dependent protein kinase 7 (CDPK)(CPK7)
Cryptosporidium parvum cgd7_3890   calcium-dependent protein kinase 7 (CDPK)(CPK7)
Neospora caninum NCLIV_016830   ULK kinase, putative
Plasmodium berghei PBANKA_1313700   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_1450000   serine/threonine protein kinase, putative
Plasmodium knowlesi PKNH_1232100   protein kinase, putative
Plasmodium vivax PVX_117985   protein kinase, putative
Plasmodium yoelii PY01077   protein Caenorhabditis elegans K07A9.2
Toxoplasma gondii TGME49_240630   ULK kinase

Essentiality

TGME49_240630 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_240630 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 24.7% 247 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 26.2% 275 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 26.7% 187 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 23.1% 286 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0033 1 1
0.0039 0.9485 0.5
0.0036 0.5 0.5
0.0022 0.5 0.5
0.0008 0.5 0.5
0.0066 0.3101 0
0.0081 1 0.5
0.0039 0.5 0.5
0.0042 0.5 0.5
0.0067 0.5 0.5
0.0004 0.5 0.5
0.0081 0.5 0.5
0.0059 1 1
0.0069 0.3067 1
0.0012 0.5 0.5
0.0027 1 0.5
0.0032 0.5 0.5
0.0091 1 0.5
0.0026 0.5 0.5
0.0092 1 0.5
0.0007 0.5 0.5
0.0003 0.5 0.5
0.0061 0.6883 0.6656
0.0039 0.5 0.5
0.0063 0.7244 0.7244
0.0062 0.6935 0
0.0018 0.5 0.5
0.0037 1 0.5
0.0029 0.5 0.5
0.0093 0.8828 0
0.0011 1 0.5
0.0023 0.5 0.5
0.0059 1 1
0.0064 0.3377 0
0.0012 0.5 0.5
0.0007 0.5 0.5
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0032 0.5 0.5
0.0059 1 1
0.0016 0.5 0.5
0.0098 0.3242 0.2614
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0063 1 1
0.0088 0.4477 1
0.0056 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_240630 (Toxoplasma gondii), ULK kinase
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