Detailed view for TGME49_265205

Basic information

TDR Targets ID: 258836
Toxoplasma gondii, WD domain, G-beta repeat-containing protein
Source Database / ID:  ToxoDB 

pI: 5.4531 | Length (AA): 1439 | MW (Da): 163510 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00400   WD domain, G-beta repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
24 323 2h14 (A) 44 332 11.00 0 1 1.01 -0.3
34 341 1jof (A) 1 356 10.00 0 1 0.72 0.57
117 668 3sfz (A) 662 1197 24.00 0.024 1 0.4158 1.28
714 904 4uos (A) 1 186 15.00 0 0.16 0.291531 -2.1
748 962 5cwm (A) 4 227 28.00 0.13 1 0.458609 -0.69
820 948 2fxo (A) 842 957 33.00 0.75 0.04 0.279846 -0.06
826 1057 1u5p (A) 1664 1871 16.00 0 0.97 0.148023 -0.4
875 1142 3o1i (A) 56 311 30.00 0.2 0.32 0.23544 0.53
1121 1288 4uos (A) 3 172 13.00 0.008 0.01 0.355948 -1.73
1128 1359 4tql (A) 9 238 14.00 0.000012 0.06 0.372423 -1.3

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite, ME49 Oocyst. Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_129382)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG4329   CG4329 gene product from transcript CG4329-RA
Echinococcus granulosus EgrG_000887100   WD repeat containing protein 65
Echinococcus multilocularis EmuJ_000887100   WD repeat containing protein 65
Giardia lamblia GL50803_16840   Trichohyalin
Homo sapiens ENSG00000243710   cilia and flagella associated protein 57
Leishmania braziliensis LbrM.16.1610   hypothetical protein, conserved
Leishmania donovani LdBPK_161650.1   hypothetical protein, conserved
Leishmania infantum LinJ.16.1650   hypothetical protein, conserved
Leishmania major LmjF.16.1550   hypothetical protein, conserved
Leishmania mexicana LmxM.16.1550   hypothetical protein, conserved
Mus musculus ENSMUSG00000028730   cilia and flagella associated protein 57
Neospora caninum NCLIV_040050   WD domain, G-beta repeat-containing protein, putative
Plasmodium berghei PBANKA_1035700   WD repeat-containing protein 65, putative
Plasmodium falciparum PF3D7_1406500   WD repeat-containing protein 65, putative
Plasmodium knowlesi PKNH_1351600   WD repeat-containing protein 65, putative
Plasmodium vivax PVX_086105   hypothetical protein, conserved
Plasmodium yoelii PY07045   hypothetical protein
Schistosoma japonicum Sjp_0131360   IPR009053,Prefoldin,domain-containing
Schistosoma japonicum Sjp_0073520   WD repeat-containing protein 65, putative
Schistosoma japonicum Sjp_0109360   Conserved hypothetical protein
Schistosoma mansoni Smp_062970   hypothetical protein
Schmidtea mediterranea mk4.011754.03  
Schmidtea mediterranea mk4.005200.01  
Schmidtea mediterranea mk4.000067.18  
Schmidtea mediterranea mk4.014204.00  
Schmidtea mediterranea mk4.036055.00  
Schmidtea mediterranea mk4.033970.00  
Schmidtea mediterranea mk4.006017.00  
Schmidtea mediterranea mk4.017690.00  
Trypanosoma brucei gambiense Tbg972.8.4680   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.4870   Component of motile flagella 6
Trypanosoma congolense TcIL3000_0_02340   DIGIT
Trypanosoma cruzi TcCLB.508837.170   hypothetical protein, conserved
Toxoplasma gondii TGME49_265205   WD domain, G-beta repeat-containing protein
Trichomonas vaginalis TVAG_214090   myosin heavy chain, putative

Essentiality

TGME49_265205 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.4870 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.4870 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.4870 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.4870 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_265205 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_265205 (Toxoplasma gondii), WD domain, G-beta repeat-containing protein
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