Detailed view for TGME49_265390

Basic information

TDR Targets ID: 258913
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 7.3008 | Length (AA): 529 | MW (Da): 58453 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF10607   CTLH/CRA C-terminal to LisH motif domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0045721   negative regulation of gluconeogenesis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 70 2kr0 (A) 194 269 39.00 0.16 0.18 0.300125 1.5
2 137 4mhc (A) 671 857 12.00 0 0.01 0.396589 -0.6
74 123 5d2q (A) 132 181 18.00 0 0.01 0.622018 -3.66
74 123 5d2s (A) 109 158 16.00 0 0 0.597018 -3.62
182 435 2of3 (A) 622 863 13.00 0 0.97 0.530651 0.27
481 528 4r7e (A) 657 700 18.00 0.67 0.2 0.172137 0.01

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_128881)

Species Accession Gene Product
Arabidopsis thaliana AT3G55070   LisH/CRA/RING-U-box domain-containing protein
Babesia bovis BBOV_III003430   hypothetical protein
Brugia malayi Bm1_02660   erythroblast macrophage protein EMP
Candida albicans CaO19.7365   weak similarity to FYV10
Caenorhabditis elegans CELE_W09G3.2   Protein MAEA-1
Dictyostelium discoideum DDB_G0284463   CT11-RanBPM domain-containing protein
Drosophila melanogaster Dmel_CG31357   CG31357 gene product from transcript CG31357-RA
Echinococcus granulosus EgrG_000079900   erythroblast macrophage protein EMP
Echinococcus multilocularis EmuJ_000079900   erythroblast macrophage protein EMP
Homo sapiens ENSG00000090316   macrophage erythroblast attacher
Loa Loa (eye worm) LOAG_07116   erythroblast macrophage protein EMP
Mus musculus ENSMUSG00000079562   macrophage erythroblast attacher
Neospora caninum NCLIV_039880   hypothetical protein
Oryza sativa 4337882   Os05g0160100
Plasmodium berghei PBANKA_1345500   conserved Plasmodium protein, unknown function
Plasmodium falciparum PF3D7_1330500   conserved Plasmodium protein, unknown function
Plasmodium knowlesi PKNH_1269900   conserved Plasmodium protein, unknown function
Plasmodium vivax PVX_082465   hypothetical protein, conserved
Plasmodium yoelii PY06135   similar to macrophage erythroblast attacher, putative
Saccharomyces cerevisiae YIL097W   glucose-induced degradation complex subunit FYV10
Schistosoma japonicum Sjp_0058650   Macrophage erythroblast attacher, putative
Schistosoma mansoni Smp_008470.2   erythroblast macrophage protein emp
Schistosoma mansoni Smp_008470.1   erythroblast macrophage protein emp
Toxoplasma gondii TGME49_265390   hypothetical protein
Theileria parva TP03_0343   hypothetical protein

Essentiality

TGME49_265390 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_265390 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_265390 (Toxoplasma gondii), hypothetical protein
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