Detailed view for TGME49_291860

Basic information

TDR Targets ID: 258977
Toxoplasma gondii, HORMA domain-containing protein
Source Database / ID:  ToxoDB 

pI: 9.3359 | Length (AA): 570 | MW (Da): 63884 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02301   HORMA domain

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
110 336 4tzq (A) 23 252 19.00 0 1 0.744746 -0.86
110 335 4tzm (A) 23 251 20.00 0 1 0.732991 -0.86
342 492 3na7 (A) 21 172 15.00 0.83 0.03 0.430412 -0.22

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_128667)

Species Accession Gene Product
Arabidopsis thaliana AT1G67370   asynaptic 1
Babesia bovis BBOV_II002160   HORMA domain containing protein
Brugia malayi Bm1_43185   Em:AC002378.1
Candida albicans CaO19.10816   DNA-binding protein, involved in meiosis
Candida albicans CaO19.3306   DNA-binding protein, involved in meiosis
Caenorhabditis elegans CELE_ZK381.1   Protein HIM-3
Cryptosporidium hominis Chro.50207   essential protein for meiotic synapsis
Cryptosporidium parvum cgd5_1750   conserved protein with N-terminal HORMA domain
Echinococcus granulosus EgrG_000163900   HORMA domain containing protein 2
Echinococcus multilocularis EmuJ_000163900   HORMA domain containing protein 2
Giardia lamblia GL50803_4084   Hop1
Homo sapiens ENSG00000176635   HORMA domain containing 2
Homo sapiens ENSG00000143452   HORMA domain containing 1
Leishmania braziliensis LbrM.35.1240   hypothetical protein, conserved
Leishmania donovani LdBPK_361170.1   HORMA domain containing protein, putative
Leishmania infantum LinJ.36.1170   hypothetical protein, conserved
Leishmania major LmjF.36.1110   hypothetical protein, conserved
Leishmania mexicana LmxM.36.1110   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_01638   hypothetical protein
Loa Loa (eye worm) LOAG_10393   hypothetical protein
Mus musculus ENSMUSG00000020419   HORMA domain containing 2
Mus musculus ENSMUSG00000028109   HORMA domain containing 1
Oryza sativa 4347514   Os09g0506800
Onchocerca volvulus OVOC1489  
Onchocerca volvulus OVOC10490  
Plasmodium berghei PBANKA_1407900   HORMA domain protein, putative
Plasmodium falciparum PF3D7_1309400   HORMA domain protein, putative
Plasmodium knowlesi PKNH_1409800   HORMA domain protein, putative
Plasmodium vivax PVX_122300   HORMA domain protein, putative
Plasmodium yoelii PY02325   HORMA domain, putative
Saccharomyces cerevisiae YIL072W   Hop1p
Schistosoma japonicum Sjp_0036110   HORMA domain-containing protein 2, putative
Schistosoma japonicum Sjp_0312300   expressed protein
Schistosoma mansoni Smp_169930   hypothetical protein
Schmidtea mediterranea mk4.001053.00  
Trypanosoma brucei gambiense Tbg972.10.6680   hypothetical protein, conserved,predicted HORMA domain protein
Trypanosoma brucei Tb927.10.5490   predicted HORMA domain protein
Trypanosoma congolense TcIL3000_10_4620   predicted HORMA domain protein
Trypanosoma cruzi TcCLB.503611.50   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506679.260   hypothetical protein, conserved
Toxoplasma gondii TGME49_291860   HORMA domain-containing protein
Theileria parva TP04_0349   hypothetical protein
Trichomonas vaginalis TVAG_230730   synaptonemal complex axial element Hop1

Essentiality

TGME49_291860 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.5490 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.5490 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.5490 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.5490 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_ZK381.1 Caenorhabditis elegans embryonic arrest wormbase
CELE_ZK381.1 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_1407900 Plasmodium berghei Dispensable plasmo
TGME49_291860 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_291860 (Toxoplasma gondii), HORMA domain-containing protein
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