Detailed view for TGME49_207390

Basic information

TDR Targets ID: 259025
Toxoplasma gondii, coatomer protein complex, gamma sub-unit, putative
Source Database / ID:  ToxoDB 

pI: 8.3904 | Length (AA): 517 | MW (Da): 56058 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00928   Adaptor complexes medium subunit family
PF01217   Clathrin adaptor complex small chain

Gene Ontology

Mouse over links to read term descriptions.
GO:0030131   clathrin adaptor complex  
GO:0005515   protein binding  
GO:0016192   vesicle-mediated transport  
GO:0006886   intracellular protein transport  
GO:0006810   transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 153 1gw5 (S) 1 142 15.00 0 0.88 0.66 -2.01
1 482 1gw5 (M) 1 434 36.00 0 1 1.22 -0.19
1 516 2jkr (M) 1 434 34.00 0 1 1.08427 0.54
2 517 4p6z (M) 3 422 35.00 0 1 1.13127 0.47
46 192 4p6z (S) 1 147 18.00 0 0.91 0.635933 -0.96
46 178 2vgl (S) 1 133 14.00 0.00000000049 1 0.577853 -0.96
246 516 1h6e (A) 169 434 38.00 0 1 0.602778 0.87

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_130982)

Species Accession Gene Product
Arabidopsis thaliana AT4G24550   clathrin adaptor complexes medium subunit family protein
Babesia bovis BBOV_II006760   clathrin coat assembly protein, putative
Cryptosporidium hominis Chro.80114   clathrin coat assembly like protein
Cryptosporidium parvum cgd8_950   clathrin coat assembly protein AP50
Dictyostelium discoideum DDB_G0276945   clathrin-adaptor medium chain AP-4
Homo sapiens ENSG00000221838   adaptor-related protein complex 4, mu 1 subunit
Mus musculus ENSMUSG00000019518   adaptor-related protein complex AP-4, mu 1
Neospora caninum NCLIV_002480   CBR-DPY-23 protein, related
Oryza sativa 4343931   Os07g0620300
Plasmodium berghei PBANKA_0928600   AP-4 complex subunit mu, putative
Plasmodium falciparum PF3D7_1119500   AP-4 complex subunit mu, putative
Plasmodium knowlesi PKNH_0917200   AP-4 complex subunit mu, putative
Plasmodium yoelii PY05839   clathrin coat assembly like protein
Trypanosoma brucei gambiense Tbg972.8.7270   mu-adaptin 4, putative,adaptor complex AP-4 medium subunit, putative
Trypanosoma brucei Tb927.8.7050   mu-adaptin 4, putative
Trypanosoma cruzi TcCLB.505073.30   mu-adaptin 4, putative
Trypanosoma cruzi TcCLB.509911.70   mu-adaptin 4, putative
Toxoplasma gondii TGME49_207390   coatomer protein complex, gamma sub-unit, putative
Theileria parva TP02_0642   hypothetical protein

Essentiality

TGME49_207390 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.7050 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.7050 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.7050 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.8.7050 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0928600 Plasmodium berghei Essential plasmo
TGME49_207390 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TGME49_207390 (Toxoplasma gondii), coatomer protein complex, gamma sub-unit, putative
Title for this comment
Comment