Detailed view for TGME49_213800

Basic information

TDR Targets ID: 259084
Toxoplasma gondii, protein phosphatase 2b regulatory subunit, putative
Source Database / ID:  ToxoDB 

pI: 4.4354 | Length (AA): 177 | MW (Da): 19995 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13499   EF-hand domain pair

Gene Ontology

Mouse over links to read term descriptions.
GO:0005509   calcium ion binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 175 1fpw (A) 1 185 27.00 0 1 1.24 -0.36
16 177 1aui (B) 8 169 53.00 0 1 1.65 -1.27
113 170 1k2h (A) 27 84 29.00 0.000029 0.55 0.82 -1.65
3 175 2ct9 (B) 4 191 37.00 0 1 1.4044 -0.63
8 176 5b8i (B) 1 169 47.00 0 1 1.6395 -1.28
16 177 1aui (B) 8 169 53.00 0.00016 1 1.62575 -0.91
23 90 1ig5 (A) 2 74 34.00 0.049 1 0.883881 -1.73
60 124 1nya (A) 102 163 32.00 0.061 0.87 0.789932 -0.69

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Bradyzoite. Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_127723)

Species Accession Gene Product
Arabidopsis thaliana AT4G16350   calcineurin B-like protein 6
Arabidopsis thaliana AT3G18430   EF-hand, calcium binding motif-containing protein
Brugia malayi Bm1_50880   calcineurin B
Candida albicans CaO19.4009   Calcineurin regulatory (B) subunit
Candida albicans CaO19.11492   Calcineurin regulatory (B) subunit
Caenorhabditis elegans CELE_F55C10.1   Protein CNB-1, isoform A
Cryptosporidium hominis Chro.10159   protein phosphatase 2B regulatory subunit
Cryptosporidium parvum cgd1_1370   protein with 3x EFh domains
Dictyostelium discoideum DDB_G0267446   CaM-dependent protein phosphatase, regulatory subunit
Drosophila melanogaster Dmel_CG11217   Calcineurin B2
Drosophila melanogaster Dmel_CG4209   Calcineurin B
Echinococcus granulosus EgrG_000454300   calcineurin b
Entamoeba histolytica EHI_106670   calcineurin B subunit, putative
Echinococcus multilocularis EmuJ_000454300   calcineurin b
Homo sapiens ENSG00000188386   protein phosphatase 3, regulatory subunit B, beta
Homo sapiens ENSG00000221823   protein phosphatase 3, regulatory subunit B, alpha
Leishmania braziliensis LbrM.21.1920   calcineurin B subunit, putative
Leishmania donovani LdBPK_211990.1   calcineurin B subunit, putative
Leishmania infantum LinJ.21.1990   calcineurin B subunit, putative
Leishmania major LmjF.21.1630   calcineurin B subunit, putative
Leishmania mexicana LmxM.21.1630   calcineurin B subunit, putative
Loa Loa (eye worm) LOAG_03379   protein phosphatase 3
Mus musculus ENSMUSG00000033953   protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)
Mus musculus ENSMUSG00000028310   protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type II)
Neospora caninum NCLIV_013540   hypothetical protein
Oryza sativa 4345696   Os08g0442300
Oryza sativa 4339432   Os05g0534400
Plasmodium berghei PBANKA_1315400   calcineurin subunit B, putative
Plasmodium falciparum PF3D7_1451700   calcineurin subunit B
Plasmodium knowlesi PKNH_1230400   calcineurin subunit B, putative
Plasmodium vivax PVX_117895   protein phosphatase 2b regulatory subunit, putative
Plasmodium yoelii PY01623   calcineurin b subunit
Saccharomyces cerevisiae YKL190W   calcineurin regulatory subunit B
Schistosoma japonicum Sjp_0035760   ko:K06268 protein phosphatase 3, regulatory subunit, putative
Schistosoma japonicum Sjp_0090050   IPR002048,Calcium-binding EF-hand,domain-containing
Schistosoma mansoni Smp_064950   calcineurin B
Schmidtea mediterranea mk4.000027.02  
Schmidtea mediterranea mk4.000949.00   CNB-1
Schmidtea mediterranea mk4.013430.01   CNB-1
Trypanosoma brucei gambiense Tbg972.10.300   calcineurin B subunit, putative
Trypanosoma brucei Tb927.10.370   calcineurin B subunit, putative
Trypanosoma congolense TcIL3000_10_230   calcineurin B subunit, putative
Trypanosoma cruzi TcCLB.506869.50   calcineurin B subunit, putative
Trypanosoma cruzi TcCLB.510519.60   calcineurin B subunit, putative
Toxoplasma gondii TGME49_213800   protein phosphatase 2b regulatory subunit, putative
Trichomonas vaginalis TVAG_475350   calcineurin B subunit, putative
Trichomonas vaginalis TVAG_079690   potassium channel interacting protein, putative
Trichomonas vaginalis TVAG_133870   DNA-dependent protein kinase catalytic subunit-interacting protein, putative
Trichomonas vaginalis TVAG_383330   calcium binding proteins, putative
Trichomonas vaginalis TVAG_425490   calcineurin B, putative
Trichomonas vaginalis TVAG_479340   calcium binding proteins, putative
Trichomonas vaginalis TVAG_295480   calcineurin B subunit, putative
Trichomonas vaginalis TVAG_021740   calcineurin B subunit, putative
Trichomonas vaginalis TVAG_066870   calcineurin B, putative

Essentiality

TGME49_213800 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.370 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.370 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.370 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.370 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1315400 Plasmodium berghei Slow plasmo
TGME49_213800 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.9

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Protein S100-B 92 aa 31.3% 83 aa Compounds References
Bos taurus Calmodulin 149 aa 30.5% 151 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_213800 (Toxoplasma gondii), protein phosphatase 2b regulatory subunit, putative
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